Retinoic Acid-Mediated G1 Arrest Is Associated with Induction of p27Kip1 and Inhibition of Cyclin-Dependent Kinase 3 in Human Lung Squamous Carcinoma CH27 Cells

2000 ◽  
Vol 258 (2) ◽  
pp. 322-331 ◽  
Author(s):  
Shih-Lan Hsu ◽  
Jen-Wen Hsu ◽  
Mei-Chun Liu ◽  
Ling-Yun Chen ◽  
Chi-Der Chang
Keyword(s):  
Retinoic Acid ◽  
Human Lung ◽  
Squamous Carcinoma ◽  
G1 Arrest ◽  
Cyclin Dependent Kinase ◽  
Lung Squamous Carcinoma

Comparative proteome analysis of human lung squamous carcinoma tissue

2006 ◽  
Vol 5 (4) ◽  
pp. 232-239 ◽  
Author(s):  
Cui Li ◽  
Can’e Tang ◽  
Chaojun Duan ◽  
Hong Yi ◽  
Zhiqiang Xiao ◽  
...  
Keyword(s):  
Human Lung ◽  
Squamous Carcinoma ◽  
Proteome Analysis ◽  
Carcinoma Tissue ◽  
Lung Squamous Carcinoma

scholarly journals Quantitative Proteomic Study of Human Lung Squamous Carcinoma and Normal Bronchial Epithelial Acquired by Laser Capture Microdissection

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Xu Yan ◽  
Cao Lan-Qin ◽  
Jin Long-Yu ◽  
Chen Zhu-Chu ◽  
Zeng Gu-Qing ◽  
...  
Keyword(s):  
Differential Expression ◽  
Laser Capture Microdissection ◽  
Human Lung ◽  
Protein Profile ◽  
Squamous Carcinoma ◽  
Bronchial Epithelium ◽  
Target Cells ◽  
Proteomic Study ◽  
Lung Squamous Carcinoma ◽  
Laser Capture

Objective. To investigate the differential protein profile of human lung squamous carcinoma (HLSC) and normal bronchial epithelium (NBE) and provide preliminary results for further study to explore the carcinogenic mechanism of HLSC.Methods. Laser capture microdissection (LCM) was used to purify the target cells from 10 pairs of HLSC tissues and their matched NHBE, respectively. A stable-isotope labeled strategy using iTRAQ, followed by 2D-LC/Q-STAR mass spectrometry, was performed to separate and identify the differential expression proteins.Results. A total of 96 differential expression proteins in the LCM-purified HLSC and NBE were identified. Compared with NBE, 49 proteins were upregulated and 47 proteins were downregulated in HLSC. Furthermore, the expression levels of the differential proteins including HSPB1, CKB, SCCA1, S100A8, as well as S100A9 were confirmed by western blot and tissue microarray and were consistent with the results of quantitative proteomics.Conclusion. The different expression proteins in HLSC will provide scientific foundation for further study to explore the carcinogenic mechanism of HLSC.

The positive feedback loop of RNASEH1-AS1/has-miR-218-5p/NET1 mediated by POU2F1 contributes to the development and progression of human lung squamous carcinoma

2020 ◽  
Author(s):  
Jing-hao Jia ◽  
Jing Wang ◽  
Jia-rui Yu ◽  
Peng Gao ◽  
Yan-kun Liu ◽  
...  
Keyword(s):  
Positive Feedback ◽  
Feedback Loop ◽  
Human Lung ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Human Lung Cancer ◽  
Migration And Invasion ◽  
Positive Feedback Loop ◽  
Squamous Carcinoma Cells ◽  
Lung Squamous Carcinoma

Abstract Background In molecular level, competing endogenous RNAs (ceRNAs) regulates other RNA transcripts through competing for shared microRNAs (miRNA). miRNA negatively regulate gene expression at the levels of mRNAs stability and translation suppression. Methods We tested the mRNA level of miR-218-5p and RNASEH1-AS1 in clinical lung squamous cell carcinoma tissues by qRT-PCR. In the exploring of the role of miR-218-5p and RNASEH1-AS1 in the malignant phenotype of NCI-H520 cells, colony formation and MTT assay were used to test the cell viability and proliferation capability, trans-well invasion and wound healing assay were performed to examine the cell migration and invasion. ChIP assay was conducted to confirm the direct interact of POU2F1 and RNASEH1-AS1 promoter. Results In this investigation, we found that LncRNA RNASEH1-AS1 is up-regulated in human lung cancer, and serves as a miRNA sponge for hsa-miR-218-5p in human lung squamous carcinoma cells. lncRNA RNASEH1-AS1 facilitates growth and motility of lung squamous carcinoma cells, while miR-218-5p does the opposite. NET1 and POU2F1 are validated as direct and functional targets of miR-218-5p. The downregulation of miR-218-5p releases the suppression of NET1 and POU2F1. POU2F1 binds directly to the lncRNA-RNASEH1-AS1 promoter and acts as transcription factor to enhance the promoter activity of RNASEH1-AS1. Conclusion Above all, the positive feedback loop of RNASEH1-AS1/ hsa-miR-218-5p/ NET1/ POU2F1 can help us to understand the regulatory mechanism behind genesis and progression of human lung squamous carcinoma, possibly providing new biomarkers for its diagnosis and treatment.

Identification of Tumor Antigens in Human Lung Squamous Carcinoma by Serological Proteome Analysis

2007 ◽  
Vol 6 (2) ◽  
pp. 751-758 ◽  
Author(s):  
Fang Yang ◽  
Zhi-qiang Xiao ◽  
Xiu-zhi Zhang ◽  
Cui Li ◽  
Peng-fei Zhang ◽  
...  
Keyword(s):  
Tumor Antigens ◽  
Human Lung ◽  
Squamous Carcinoma ◽  
Proteome Analysis ◽  
Lung Squamous Carcinoma

scholarly journals Effector mechanism of magnolol-induced apoptosis in human lung squamous carcinoma CH27 cells

2003 ◽  
Vol 138 (1) ◽  
pp. 193-201 ◽  
Author(s):  
Shu-Er Yang ◽  
Ming-Tsuen Hsieh ◽  
Tung-Hu Tsai ◽  
Shih-Lan Hsu
Keyword(s):  
Human Lung ◽  
Squamous Carcinoma ◽  
Effector Mechanism ◽  
Lung Squamous Carcinoma ◽  
Induced Apoptosis

Identificating 14-3-3 sigma as a lymph node metastasis-related protein in human lung squamous carcinoma

2009 ◽  
Vol 279 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Dan-juan Li ◽  
Gui Deng ◽  
Zhi-qiang Xiao ◽  
Hui-xin Yao ◽  
Cui Li ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Human Lung ◽  
Squamous Carcinoma ◽  
Related Protein ◽  
Node Metastasis ◽  
Lung Squamous Carcinoma

scholarly journals The positive RNASEH1-AS1/has-miR-218-5p/NET1 feedback loop mediated by POU2F1 contributes to the development and progression of human lung squamous carcinoma

2020 ◽  
Author(s):  
Jing-hao Jia ◽  
Jing Wang ◽  
Jia-rui Yu ◽  
Peng Gao ◽  
Yan-kun Liu ◽  
...  
Keyword(s):  
Feedback Loop ◽  
Human Lung ◽  
Lung Squamous Cell Carcinoma ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Human Lung Cancer ◽  
Migration And Invasion ◽  
Squamous Carcinoma Cells ◽  
Lung Squamous Carcinoma ◽  
New Biomarkers

Abstract Background At the molecular level, competing endogenous RNAs (ceRNAs) regulate other RNA transcripts by competing for shared microRNAs (miRNA). Notably, miRNAs negatively regulate gene expression at the levels of mRNA stability and translation suppression.MethodsWe measured theexpression of miR-218-5p and RNASEH1-AS1 in clinical lung squamous cell carcinoma tissues using qRT-PCR. In an attempt to explore the roles of miR-218-5p and RNASEH1-AS1 in determining the malignant phenotype of NCI-H520 cells, colony formation and MTT assays were performed to measure cell viability and proliferation, and transwell invasion and wound healing assays were performed to examine cell migration and invasion. A ChIP assay was conducted to confirm the direct binding of POU2F1 to the RNASEH1-AS1 promoter.ResultsIn this investigation, the expression of the lncRNA RNASEH1-AS1 is upregulated in human lung cancer tissues, and it functions as a miRNA sponge for hsa-miR-218-5p in human lung squamous carcinoma cells. The lncRNA RNASEH1-AS1 facilitates the growth and motility of lung squamous carcinoma cells, while miR-218-5p exerts the opposite effects. NET1 and POU2F1 are validated as direct and functional targets of miR-218-5p. The downregulation of miR-218-5p releases the suppression of NET1 and POU2F1. POU2F1 binds directly to the lncRNA-RNASEH1-AS1 promoter and functions as transcription factor to enhance the promoter activity of RNASEH1-AS1.ConclusionsOverall, the positive RNASEH1-AS1/hsa-miR-218-5p/NET1/POU2F1 feedback loop can help us understand the regulatory mechanism underlying the genesis and progression of human lung squamouscarcinoma, possibly providing new biomarkers for its diagnosis and treatment.

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