A Simple Method for Accurate Estimation of Apoptotic Cells

2000 ◽  
Vol 256 (1) ◽  
pp. 328-337 ◽  
Author(s):  
Narendra P. Singh
2012 ◽  
Vol 2012 (1) ◽  
pp. 001081-001084 ◽  
Author(s):  
Jesse Bowman ◽  
A. Ege Engin

When integrating sensitive RF analog devices with complex VLSI digital components, simultaneously switching drivers cause supply voltage fluctuations which can propagate both horizontally and vertically between the power/ground planes. The same voltage source on a printed circuit board can be shared to increase power efficiency and reduce space used. In order to accomplish this, on board filtering is needed to isolate the noise between these two types of devices for proper operation. Hence, accurate estimation and improvement of the performance of power/ground planes is critical in a mixed-signal system. We present a new method to minimize the noise transfer at high frequencies to the power distribution system, called the Virtual Ground Fence. At its basic level, the Virtual Ground Fence consists of quarter-wave transmission-line stubs that act as short circuits between power and ground planes at their design frequency. We will present various configurations of Virtual Ground Fence for different coupling scenarios.


2007 ◽  
Vol 85 (7) ◽  
pp. 652-658 ◽  
Author(s):  
B.B. Tóth ◽  
A. Feest

The methodologies used for studying macrofungal communities are an eclectic assemblage of many different and unstandardized approaches. In this study we propose a simple, nondestructive but still informative method to assess fungal sporocarp biomass in forested areas. We use the number of fruiting bodies found in the surveyed plot, and data on cap diameter of the species from the literature to calculate a cap area index. We show that this index very strongly correlates with the measured total dry mass of the species obtained from the same plot. We point out that the cap area index provides a more accurate estimation of the epigeous fungal sporocarp biomass than do the species sporocarp numbers. This new methodology allows the spatiotemporal distribution of a fungal community in an ecosystem to be followed. Since sporocarp production responds much more sensitively to environmental changes than does the fine-root vegetative ectomycorrhizal community, it may more quickly reflect whether an ecosystem has been perturbed. It will also allow us to collect data on saprotrophic species. A sporocarp survey can be useful for detecting early changes in the habitat and environment, and give an easily applicable method for conservation biology and ecosystem management.


2012 ◽  
Vol 52 (2) ◽  
pp. 640
Author(s):  
Mike Jones

The estimation of product yields from gas and gas condensate reservoirs is often the subject of multi-million-dollar studies, requiring gas and condensate samples from production tests, lab analysis of the samples, and complex process engineering models. An accurate estimation of sales product yields can, however, be determined simply from the composition of a reservoir sample and a basic material balance calculation. Sales gas, LNG, and condensate have fairly consistent specifications across the world, based on various properties such as heating value, vapour pressure, etc. This consistency allows the determination of product yields from simple material balance calculations and properties of the individual components found in the reservoir gas. In this case, material balance simply means the allocation of each component to a particular product stream. The lighter components, C1 through C4 (methane through butane), comprise the LNG and/or sales gas product, and all C5+ (pentane and heavier) components make up the condensate product. The yields can then calculated for each unit of reservoir gas, for example MJ/scm, BTU/scf, bbl/MMscf, etc. Inerts such as CO2 and N2 have no heating value and are not included in the yield calculation. Likewise, contaminants such as H2S must be removed from the product stream and are not included in the yield. In actual practice, a perfect separation of the individual components is not achieved—that is,the condensate product will contain small amounts of C3 and C4, but experience has shown that the simple method described above gives an accurate estimattion of product yields from a simple gas analysis.


1994 ◽  
Vol 116 (2) ◽  
pp. 162-167 ◽  
Author(s):  
H. M. Shang ◽  
M. Lwin ◽  
T. E. Tay

Circular plates, under unknown clamping conditions and containing simulated defects in the form of circular localized thinning or thickening, are inspected by double-exposure holography. With an incremental uniform pressure applied between exposures, eccentric defects are readily revealed from the distinct irregular fringe patterns. In the case of central circular defects, however, the absence of distinct irregular fringe patterns does not enable easy visual detection of the defects. The simple method of analysis described in this paper, based on the fact that the displacement in a defective plate differs from that in a defect-free plate, allows easy deduction of central and eccentric defects from the fringe patterns. Furthermore, this method enables identification of the type of defect (localized thinning or thickening), the extent of thickness variation, as well as an accurate estimation of the location and size of the defect.


1992 ◽  
Author(s):  
M. B. Okan ◽  
D. G. Gregory-Smith

Reasonably accurate estimation of losses at an early design stage plays an important part in the success of a turbine design. Although various computational methods exist for estimating the profile loss, for secondary losses which are equally important, designers still rely on emprical estimates. A method has been developed for estimating the secondary flow and secondary losses within an axial turbine cascade. It is assumed that the inlet boundary layer is convected into a loss core within the blade passage while extra loss has been generated due to the secondary kinetic energy and a new boundary layer that is developing on the end wall. The method has been applied to various test cases and the results show that the basic approach is reliable.


2017 ◽  
Vol 9 (7) ◽  
pp. 168781401771370 ◽  
Author(s):  
Xiao-Ming Zhou ◽  
Zhi-Kun Wang ◽  
Yi-Fang Zhang

Flow coefficient is an important performance index associated with the energy efficiency of a valve, and an effective method to evaluate valve flow coefficient is necessary for valve industry. However, theoretical estimation often results in poor accuracy, while experimental measurements involve significant costs in time and equipment. In this article, a computational fluid dynamics method is proposed to achieve simple and accurate evaluation of valve flow coefficient. For each valve, a computational fluid dynamics model is established containing a valve section, an upstream section, and a downstream section. A grid-adaptation strategy is then applied to improve the accuracy of simulation. To calculate flow coefficient, the most important issue is to determine the net pressure loss induced by valve (Δ Pv). Herein, the overall pressure drop (Δ Po) is obtained first, and the pipe-induced pressure drop (Δ Pp) is estimated by linear fitting. Then, Δ Pv is calculated as the difference between Δ Po and Δ Pp. To ensure accurate estimation of the pressure losses, a length of 26 times of pipe diameter is preferred for the upstream section. The experiments demonstrated that the presented method can accurately predict flow coefficient for various types of valves and thus has great potential to be widely used in the valve industry.


Blood ◽  
2000 ◽  
Vol 96 (4) ◽  
pp. 1388-1392 ◽  
Author(s):  
Vilasini Shetty ◽  
Seema Hussaini ◽  
LaTanya Broady-Robinson ◽  
Krishnan Allampallam ◽  
Suneel Mundle ◽  
...  

Abstract A higher percentage of apoptotic cells (apoptotic index or AI) is consistently found in bone marrow (BM) biopsies compared to BM aspirates of patients with myelodysplastic syndrome (MDS). Most studies have only investigated the low-density fraction (LDF) mononuclear cells from BM aspirates following density separation for AI determination. In the present study, both LDF and high-density fraction (HDF) cells for AI were examined by electron microscopy (EM) in 10 MDS patients and 4 healthy donors. Matched BM biopsies were subjected to AI detection by in situ end labeling (ISEL) of fragmented DNA. The results indicate that in LDF and HDF cells, AI is consistently higher in MDS patients (8.5% vs 1.5%, respectively; P = .039) compared to healthy donors (27% vs 4%, respectively; P = .004). The BM biopsy AI was also higher in MDS patients than in healthy donors (3+ vs 0+, respectively; P = .036). In addition, in MDS patients, more apoptotic cells were found in HDF cells than in LDF cells (27% vs 8.5%, respectively;P = .0001). All stages of maturation, ranging from blasts to terminally mature cells belonging to all 3 lineages, were represented in the dying cells in both compartments. Using EM, typical Pelger-Huett–type cells appeared to be apoptotic granulocytes. Both LDF and HDF cells should be examined for an accurate estimation of apoptotic cells because AI would be underestimated if only the LDF cells were studied. Ultrastructural studies consistently show a higher AI in BM biopsies compared to BM aspirates despite the correction factor of HDF cells provided by AI. This may represent the actual extant state, which could conceivably be due to a higher concentration of proapoptotic signals in the biopsies.


MAUSAM ◽  
2022 ◽  
Vol 44 (3) ◽  
pp. 239-242
Author(s):  
H.P. DAS ◽  
A.D. PUJARI

Solar radiation is or vital interest in characterizing an area with respect to its agricultural potential. However, these are not readily available for a large network. An attempt. has been made to deduce solar irradiance from climatic data, such as temperature range.   Based on daily data of Pune for 1986-90, a relationship has been developed between atmospheric transmittance and the daily range of air temperature. The model developed has been tested on independent data and found to give fairly accurate estimation of solar irradiance. Nearly 70% of the variation in daily solar radiation could be explained by this simple method. The effect of solar irradiance on microclimate has also been discussed. The model developed has been tested for Hyderabad and Calcutta and found to give encouraging results.


Blood ◽  
2000 ◽  
Vol 96 (4) ◽  
pp. 1388-1392
Author(s):  
Vilasini Shetty ◽  
Seema Hussaini ◽  
LaTanya Broady-Robinson ◽  
Krishnan Allampallam ◽  
Suneel Mundle ◽  
...  

A higher percentage of apoptotic cells (apoptotic index or AI) is consistently found in bone marrow (BM) biopsies compared to BM aspirates of patients with myelodysplastic syndrome (MDS). Most studies have only investigated the low-density fraction (LDF) mononuclear cells from BM aspirates following density separation for AI determination. In the present study, both LDF and high-density fraction (HDF) cells for AI were examined by electron microscopy (EM) in 10 MDS patients and 4 healthy donors. Matched BM biopsies were subjected to AI detection by in situ end labeling (ISEL) of fragmented DNA. The results indicate that in LDF and HDF cells, AI is consistently higher in MDS patients (8.5% vs 1.5%, respectively; P = .039) compared to healthy donors (27% vs 4%, respectively; P = .004). The BM biopsy AI was also higher in MDS patients than in healthy donors (3+ vs 0+, respectively; P = .036). In addition, in MDS patients, more apoptotic cells were found in HDF cells than in LDF cells (27% vs 8.5%, respectively;P = .0001). All stages of maturation, ranging from blasts to terminally mature cells belonging to all 3 lineages, were represented in the dying cells in both compartments. Using EM, typical Pelger-Huett–type cells appeared to be apoptotic granulocytes. Both LDF and HDF cells should be examined for an accurate estimation of apoptotic cells because AI would be underestimated if only the LDF cells were studied. Ultrastructural studies consistently show a higher AI in BM biopsies compared to BM aspirates despite the correction factor of HDF cells provided by AI. This may represent the actual extant state, which could conceivably be due to a higher concentration of proapoptotic signals in the biopsies.


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