Interferon-γ Inhibits Insulin Release and Induces Cell Death in the Pancreatic β-Cell Line INS-1 Independently of Nitric Oxide Production

1997 ◽  
Vol 237 (1) ◽  
pp. 217-222 ◽  
Author(s):  
R LAFFRANCHI ◽  
G SPINAS
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Cell Line ◽  
Insulin Release ◽  
Interferon Γ ◽  
Nitric Oxide Production ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
Oxide Production

Interleukin 10 inhibits insulin release from and nitric oxide production in rat pancreatic islets

1996 ◽  
Vol 135 (3) ◽  
pp. 374-378 ◽  
Author(s):  
Renato Laffranchi ◽  
Giatgen A Spinas
Keyword(s):  
Nitric Oxide ◽  
Pancreatic Islets ◽  
Insulin Release ◽  
Interleukin 10 ◽  
Interleukin 1Β ◽  
Nitric Oxide Production ◽  
Β Cell ◽  
Cell Dysfunction ◽  
Rat Pancreatic Islets ◽  
Oxide Production

Laffranchi R, Spinas GA. Interleukin 10 inhibits insulin release from and nitric oxide production in rat pancreatic islets. Eur J Endocrinol 1996;135:374–8. ISSN 0804–4643 Interleukin 10 was found to prevent cytokine-induced nitric oxide production in murine macrophages. Because, in rat islets, interleukin 1β induces β-cell dysfunction, mainly due to overproduction of nitric oxide, we studied if this effect could be counteracted by interleukin 10. Rat pancreatic islets were cultured for 24 h in the presence or absence of 0.02–20 ng/ml recombinant human interleukin 10. Interleukin 10 dose-dependently inhibited insulin secretion with maximal inhibition (27 ±4%, p < 0.05) at 2 ng/ml without impairment of islet cell viability. However, incubation of pancreatic islets with interleukin 10 resulted in a 61.5% decrease of nitric oxide production. Co-incubation of islets with interleukin 10 (2 ng/ml) and recombinant human interleukin 1β (0.15 ng/ml) resulted in a more pronounced suppression of basal insulin release than with interleukin 1β alone (55 ± 3.6% vs 44 ± 3.6% with interleukin 1β alone, p < 0.05) but did not reduce interleukin 1β-stimulated NO production or reverse the effect of interleukin 1β on cell viability. Thus, in pancreatic islets interleukin 10 is not capable of counteracting the interleukin 1β-induced β-cell dysfunction, but rather enhances the inhibitory effect of interleukin 1β by a different mechanism. Renato Laffranchi, Division of Endocrinology and Metabolism, Department of Internal Medicine, University Hospital, Rämistrasse 100, CH-8091 Zürich, Switzerland

Gamma Interferon-Induced Nitric Oxide Production in Mouse CD5+B1-Like Cell Line and Its Association with Apoptotic Cell Death

2003 ◽  
Vol 47 (9) ◽  
pp. 669-679 ◽  
Author(s):  
Naoki Koide ◽  
Tsuyoshi Sugiyama ◽  
Mya Mya Mu ◽  
Isamu Mori ◽  
Tomoaki Yoshida ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Cell Line ◽  
Apoptotic Cell ◽  
Gamma Interferon ◽  
Apoptotic Cell Death ◽  
Nitric Oxide Production ◽  
Oxide Production

Pentoxifylline Potentiates Nitric Oxide Production and Growth Suppression in Interferon-γ-Treated L929 Fibroblasts

1998 ◽  
Vol 184 (2) ◽  
pp. 105-111 ◽  
Author(s):  
Stanislava Stošić-Grujičić ◽  
Vladimir Trajković ◽  
Vladimir Badovinac ◽  
Marija Mostarica Stojković
Keyword(s):  
Nitric Oxide ◽  
Interferon Γ ◽  
Growth Suppression ◽  
Nitric Oxide Production ◽  
Oxide Production

scholarly journals Prostaglandin E2potentiates interferon-γ-induced nitric oxide production in cultured rat microglia

2017 ◽  
Vol 140 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Takayuki Nagano ◽  
Ryo Nishiyama ◽  
Ayaka Sanada ◽  
Yukiko Mutaguchi ◽  
Anna Ioku ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Interferon Γ ◽  
Nitric Oxide Production ◽  
Oxide Production
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