Involvement of Nitric Oxide in Permeability Alteration and F-Actin Redistribution Induced by Phorbol Myristate Acetate in Endothelial Cells

1995 ◽  
Vol 221 (2) ◽  
pp. 289-293 ◽  
Author(s):  
Shu Ming Liu ◽  
Tommy Sundqvist
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Phorbol Myristate Acetate ◽  
Myristate Acetate ◽  
Permeability Alteration

Contrasting effects of inflammatory stimuli on neutrophil and monocyte adherence to endothelial cells

1989 ◽  
Vol 67 (2) ◽  
pp. 556-562 ◽  
Author(s):  
D. W. Kamp ◽  
K. D. Bauer ◽  
A. Knap ◽  
M. M. Dunn
Keyword(s):  
Hydrogen Peroxide ◽  
Endothelial Cells ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Phorbol Myristate Acetate ◽  
Superoxide Anion ◽  
Leukocyte Adherence ◽  
Myristate Acetate ◽  
Kinase C ◽  
Monocyte Adherence

Leukocyte adherence to endothelial cells (EC) is an important early event in inflammatory responses, which are often characterized by a predominance of either neutrophils (PMN) or monocytes. However, there is little information concerning the molecular events important in leukocyte adherence to EC. Intracellular activation of protein kinase C and the calcium-second messenger system leads to the stimulation of a number of important functions in PMN and monocytes. We compared the effects of members of these pathways on human PMN and monocyte adherence to cultured bovine aortic EC. We observed that phorbol myristate acetate, phorbol, 12,13-dibutyrate, L-alpha-1-oleoyl-2-acetoyl-sn-3-glycerol, and ionomycin each induced significant dose-dependent increases in PMN adherence to EC monolayers. In contrast, similar concentrations of each of these agents induced significant decreases in EC adherence of monocytes enriched by countercurrent centrifugal elutriation. Separate experiments determined that the differences in PMN and monocyte adherence to EC were not related to differences in oxidant production because 1) phorbol myristate acetate and L-alpha-1-oleoyl-2-acetoyl-sn-3-glycerol caused similar marked increases in both PMN and monocyte superoxide anion and hydrogen peroxide production and 2) ionomycin, which had opposing effects on PMN and monocyte adherence, had no effect on PMN and monocyte superoxide anion or hydrogen peroxide release. We conclude that activators of protein kinase C and the Ca-second messenger pathway have opposite effects on PMN and monocyte adherence to EC and that these effects are mediated by O2 radical-independent mechanisms.(ABSTRACT TRUNCATED AT 250 WORDS)

Phorbol myristate acetate induces differentiation of THP-1 cells in a nitric oxide-dependent manner

Nitric Oxide ◽  
2021 ◽  
Vol 109-110 ◽  
pp. 33-41
Author(s):  
Ya-Ying Chang ◽  
Cheng-Wei Lu ◽  
Wei-Horng Jean ◽  
Jiann-Shing Shieh ◽  
Tzu-Yu Lin
Keyword(s):  
Nitric Oxide ◽  
Phorbol Myristate Acetate ◽  
Dependent Manner ◽  
Myristate Acetate

scholarly journals Endothelial diaphragmed fenestrae: in vitro modulation by phorbol myristate acetate.

1986 ◽  
Vol 102 (5) ◽  
pp. 1965-1970 ◽  
Author(s):  
T Lombardi ◽  
R Montesano ◽  
M B Furie ◽  
S C Silverstein ◽  
L Orci
Keyword(s):  
Endothelial Cells ◽  
Cell Surface ◽  
Phorbol Myristate Acetate ◽  
Freeze Fracture ◽  
Tumor Promoter ◽  
Microvascular Endothelial Cells ◽  
Myristate Acetate ◽  
Capillary Endothelial Cells

Cultured microvascular endothelial cells isolated from fenestrated capillaries have been shown to express many properties of their in vivo differentiated phenotype, yet they contain very few diaphragmed fenestrae. We show here that treatment of capillary endothelial cells with the tumor promoter, 4 beta-phorbol 12-myristate 13-acetate, induces more than a fivefold increase in the frequency of fenestrae per micron 2 of cell surface, as determined from a quantitative evaluation on freeze-fracture replicas. In quick-frozen, deep-etched preparations, the endothelial fenestrae appeared to be bridged by a diaphragm composed of radial fibers interweaving in a central mesh, as previously observed in vivo. These results indicate that diaphragmed fenestrae are inducible structures, and provide an opportunity to study them in vitro.

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