Ganglioside GT1b Induces Keratinocyte Differentiation without Activating Protein Kinase C

1995 ◽  
Vol 217 (1) ◽  
pp. 118-124 ◽  
Author(s):  
Amy S. Paller ◽  
Sheryl L. Arnsmeier ◽  
Gary J. Fisher ◽  
Qian-Chun Yu
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Keratinocyte Differentiation ◽  
Kinase C ◽  
Ganglioside Gt1b

Foxn1 promotes keratinocyte differentiation by regulating the activity of protein kinase C

2007 ◽  
Vol 75 (8) ◽  
pp. 694-701 ◽  
Author(s):  
Jian Li ◽  
Ruth M. Baxter ◽  
Lorin Weiner ◽  
Paul F. Goetinck ◽  
Enzo Calautti ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Keratinocyte Differentiation ◽  
Kinase C

Protein Kinase C  (PKC ): Its Involvement in Keratinocyte Differentiation

2002 ◽  
Vol 132 (6) ◽  
pp. 853-857 ◽  
Author(s):  
M. Kashiwagi ◽  
M. Ohba ◽  
K. Chida ◽  
T. Kuroki
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Keratinocyte Differentiation ◽  
Kinase C

scholarly journals ADAM17/EGFR axis promotes transglutaminase-dependent skin barrier formation through phospholipase C γ1 and protein kinase C pathways

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Cristina Wolf ◽  
Yawen Qian ◽  
Matthew A. Brooke ◽  
David P. Kelsell ◽  
Claus-Werner Franzke
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Skin Diseases ◽  
Skin Barrier ◽  
Epidermal Differentiation ◽  
Keratinocyte Differentiation ◽  
Inflammatory Skin Diseases ◽  
Cholesterol Sulfate ◽  
Kinase C ◽  
Barrier Formation

Abstract The vitally important skin barrier is formed by extensive cross-linking activity of transglutaminases (TGs) during terminal epidermal differentiation. We have previously shown that epidermal deficiency of a disintegrin and metalloproteinase 17 (ADAM17), the principal EGFR ligand sheddase, results in postnatal skin barrier defects in mice due to impeded TG activity. However, the mechanism by which ADAM17/EGFR signalling maintains TG activity during epidermal differentiation remains elusive. Here we demonstrate that ADAM17-dependent EGFR signalling promotes TG activity in keratinocytes committed to terminal differentiation by direct induction of TG1 expression. Restored TG1 expression of EGF-stimulated differentiated Adam17 −/− keratinocytes was strongly repressed by inhibitors for PLCγ1 or protein kinase C (PKC) pathways, while treatment with the PKC stimulator 12-O-tetradecanoylphorbol-13-acetate restored TG activity in the epidermis of keratinocyte-specific Adam17 −/− (AD17 ΔKC ) mice. Further investigations emphasized the expression of PKCη, a mediator of TGM1 transcription, to be sensitive to EGFR activation. In agreement, topical skin application of cholesterol sulfate, an activator of PKCη, significantly improved TG activity in epidermis of AD17 ΔKC mice. Our results suggest ADAM17/EGFR-driven PLCγ1 and PKC pathways as important promoters of TG1 expression during terminal keratinocyte differentiation. These findings may help to identify new therapeutic targets for inflammatory skin diseases related to epidermal barrier defects.

scholarly journals Subtype-specific targeting of protein kinase C in keratinocyte differentiation

2000 ◽  
Vol 82 ◽  
pp. 114
Author(s):  
Chihiro Otsuji ◽  
Yasuhito Shirai ◽  
Kaori Kashiwagi ◽  
Norio Sakai ◽  
Kazuhiro Chida ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Keratinocyte Differentiation ◽  
Specific Targeting ◽  
Kinase C
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