Segment Specificity of the Cytological Response in Rainbow Trout (Oncorhynchus mykiss) Renal Tubules Following Prolonged Exposure to Sublethal Concentrations of Atrazine

1995 ◽  
Vol 32 (1) ◽  
pp. 39-50 ◽  
Author(s):  
Y. Oulmi ◽  
R.D. Negele ◽  
T. Braunbeck
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Prolonged Exposure ◽  
Renal Tubules ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Sublethal Concentrations

Physiological Responses of Rainbow Trout (Oncorhynchus mykiss) to Prolonged Exposure to Soft Water

1996 ◽  
Vol 69 (6) ◽  
pp. 1419-1441 ◽  
Author(s):  
S. F. Perry ◽  
S. G. Reid ◽  
E. Wankiewicz ◽  
V. Iyer ◽  
K. M. Gilmour
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Prolonged Exposure ◽  
Physiological Responses ◽  
Soft Water ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals Effects of exposure to three environmental chemicals on the selected biochemical parameters of the blood plasma of rainbow trout, Oncorhynchus mykiss (Walbaum)

2016 ◽  
Vol 65 (1) ◽  
pp. 15-32 ◽  
Author(s):  
Jiří Řehulka ◽  
Bohumil Minařík ◽  
Miroslav Machala
Keyword(s):  
Rainbow Trout ◽  
Bile Duct ◽  
Oncorhynchus Mykiss ◽  
Histological Examination ◽  
Mineral Metabolism ◽  
Environmental Chemicals ◽  
Renal Tubules ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Activity Of Enzymes ◽  
Negative Controls

AbstractRainbow trout Oncorhynchus mykiss at a weight of 115 ± 24 g (mean ± SD) were experimentally injected with di(2-ethylhexyl)phthalate (DEHP), 2,3,7,8-tetrachlorodibenzo-p-dioxine (TCDD) and gamma isomer of hexachlorocyclohexane (γ-HCH ) to evaluate nitrogen metabolism (total protein [TP], blood urea nitrogen, uric acid [UA], creatinine), carbohydrate metabolism (glucose), mineral metabolism (inorganic phosphate[P], total calcium [Cat]), and catalytic activity of enzymes (alanine aminotransferase [ALT], aspartate aminotransferase [AST], alkaline phosphatase [ALP], lactate dehydrogenase [LD]). After 21 days, the tested xenobiotics, administered by the intraperitoneally route, caused the following effect: in comparison with negative controls, fish injected with DEHP at concentrations of 200 or 50 mg kg-1of body weight were found to have a higher level of P and lower level of Catand decreased catalytic concentrations of ALT, AST, ALP and LD in the plasma of the peripheral blood. A higher level of TP and P and a decreased catalytic concentration of ALT were found when TCDD was administered at a dose of 2 μg kg-1. A decrease in the catalytic concentrations of ALT and ALP occurred in fish injected with γ-HCH in a dose of 50 mg kg-1. UA and P levels were decreased in fish injected with γ-HCH in a dose of 5 mg kg-1. DEHP and TCDD caused neutrophilic leucocytosis with a marked left shift. Both concentrations of γ-HCH led to an increase in polychromatophilic erythrocytes. The frequency of micronuclei varied in all experimental groups, including the controls, ranging between 0.8 and 2.1 ‰. Histological examination in fish injected with DEHP at a concentration of 200 mg kg-1revealed eosinophilic droplets in the epithelium of renal tubules and histological examination in fish injected with γ-HCH at a higher concentration revealed an increased activation of sinusoidal cells in the liver, a fibrous thickening of bile duct walls, bile duct hyperplasia and a periductal inflammatory.

Renal expression and localization of SLC9A3 sodium/hydrogen exchanger and its possible role in acid-base regulation in freshwater rainbow trout (Oncorhynchus mykiss)

2008 ◽  
Vol 295 (3) ◽  
pp. R971-R978 ◽  
Author(s):  
Goran Ivanis ◽  
Marvin Braun ◽  
Steve F. Perry
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Transcriptional Activation ◽  
Plasma Cortisol ◽  
Renal Tubules ◽  
Acid Base ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Sodium Hydrogen Exchanger ◽  
Renal Expression

Experiments were performed to assess the possible involvement of the Na+/H+ exchanger isoform 3 (NHE3; SLC9A3) in renal acid-base regulation in adult rainbow trout ( Oncorhynchus mykiss). NHE3 mRNA was expressed at high levels in the kidney relative to its paralog, NHE2. The results of in situ hybridization demonstrated an abundance of NHE3 mRNA in renal tubules. The combination of immunocytochemistry and histological staining revealed that NHE3 was confined to the apical membrane of proximal tubules, where it was colocalized with the vacuolar-type H+-ATPase. Levels of NHE3 protein (assessed by Western blotting) were increased during hypercapnia, likely as a result of increased transcription, as indicated by increasing levels of NHE3 mRNA (as determined by real-time PCR). Plasma cortisol concentration was increased during hypercapnia, and administration of exogenous cortisol caused a marked increase in NHE3 mRNA and protein. Thus we speculate that the elevation of plasma cortisol during hypercapnia contributes to transcriptional activation of NHE3 that ultimately promotes acid-base regulation by stimulating H+ secretion and HCO3− reabsorption.

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