scholarly journals Twist Plays an Essential Role in FGF and SHH Signal Transduction during Mouse Limb Development

2002 ◽  
Vol 248 (1) ◽  
pp. 143-156 ◽  
Author(s):  
Meredith P. O'Rourke ◽  
Kenneth Soo ◽  
Richard R. Behringer ◽  
Chi-Chung Hui ◽  
Patrick P.L. Tam
Keyword(s):  
Signal Transduction ◽  
Limb Development ◽  
Essential Role ◽  
Mouse Limb

Products, genetic linkage and limb patterning activity of a murine hedgehog gene

Development ◽  
1994 ◽  
Vol 120 (11) ◽  
pp. 3339-3353 ◽  
Author(s):  
D.T. Chang ◽  
A. Lopez ◽  
D.P. von Kessler ◽  
C. Chiang ◽  
B.K. Simandl ◽  
...  
Keyword(s):  
Limb Development ◽  
Genetic Linkage ◽  
Pcr Primers ◽  
Amino Acid Identity ◽  
Protein Product ◽  
Proteolytic Processing ◽  
Limb Bud ◽  
Gene Encoding ◽  
Mouse Limb ◽  
Drosophila Embryos

The hedgehog (hh) segmentation gene of Drosophila melanogaster encodes a secreted signaling protein that functions in the patterning of larval and adult structures. Using low stringency hybridization and degenerate PCR primers, we have isolated complete or partial hh-like sequences from a range of invertebrate species including other insects, leech and sea urchin. We have also isolated three mouse and two human DNA fragments encoding distinct hh-like sequences. Our studies have focused upon Hhg-1, a mouse gene encoding a protein with 46% amino acid identity to hh. The Hhg-1 gene, which corresponds to the previously described vhh-1 or sonic class, is expressed in the notochord, ventral neural tube, lung bud, hindgut and posterior margin of the limb bud in developing mouse embryos. By segregation analysis the Hhg-1 gene has been localized to a region in proximal chromosome 5, where two mutations affecting mouse limb development previously have been mapped. In Drosophila embryos, ubiquitous expression of the Hhg-1 gene yields effects upon gene expression and cuticle pattern similar to those observed for the Drosophila hh gene. We also find that cultured quail cells transfected with a Hhg-1 expression construct can induce digit duplications when grafted to anterior or mid-distal but not posterior borders within the developing chick limb; more proximal limb element duplications are induced exclusively by mid-distal grafts. Both in transgenic Drosophila embryos and in transfected quail cells, the Hhg-1 protein product is cleaved to yield two stable fragments from a single larger precursor. The significance of Hhg-1 genetic linkage, patterning activity and proteolytic processing in Drosophila and chick embryos is discussed.

slimb coordinates wg and dpp expression in the dorsal-ventral and anterior-posterior axes during limb development

Development ◽  
1998 ◽  
Vol 125 (17) ◽  
pp. 3411-3416 ◽  
Author(s):  
N.A. Theodosiou ◽  
S. Zhang ◽  
W.Y. Wang ◽  
T. Xu
Keyword(s):  
Genetic Analysis ◽  
Limb Development ◽  
Imaginal Disc ◽  
Essential Role ◽  
Present Evidence ◽  
First Time ◽  
Anterior Posterior

In the Drosophila leg disc, wingless (wg) and decapentaplegic (dpp) are expressed in a ventral-anterior and dorsal-anterior stripe of cells, respectively. This pattern of expression is essential for proper limb development. While the Hedgehog (Hh) pathway regulates dpp and wg expression in the anterior-posterior (A/P) axis, mechanisms specifying their expression in the dorsal-ventral (D/V) axis are not well understood. We present evidence that slimb mutant clones in the disc deregulate wg and dpp expression in the D/V axis. This suggests for the first time that their expression in the D/V axis is actively regulated during imaginal disc development. Furthermore, slimb is unique in that it also deregulates wg and dpp in the A/P axis. The misexpression phenotypes of slimb- clones indicate that the regulation of wg and dpp expression is coordinated in both axes, and that slimb plays an essential role in integrating A/P and D/V signals for proper patterning during development. Our genetic analysis further reveals that slimb intersects the A/P pathway upstream of smoothened (smo).

scholarly journals Sim1 and Sim2 expression during chick and mouse limb development

2009 ◽  
Vol 53 (1) ◽  
pp. 149-157 ◽  
Author(s):  
Pascal Coumailleau ◽  
Delphine Duprez
Keyword(s):  
Limb Development ◽  
Mouse Limb

WITHDRAWN: A screen for recessive mutations affecting mouse limb development

2007 ◽  
Author(s):  
Kathryn V. Anderson ◽  
Lee A. Niswander ◽  
Scott D. Weatherbee
Keyword(s):  
Limb Development ◽  
Recessive Mutations ◽  
Mouse Limb

scholarly journals Evidence for genetic control of Sonic hedgehog by Gli3 in mouse limb development

1997 ◽  
Vol 62 (2) ◽  
pp. 175-182 ◽  
Author(s):  
Dirk Büscher ◽  
Birgit Bosse ◽  
Joachim Heymer ◽  
Ulrich Rüther
Keyword(s):  
Genetic Control ◽  
Sonic Hedgehog ◽  
Limb Development ◽  
Mouse Limb

Serrate2 is disrupted in the mouse limb-development mutant syndactylism

Nature ◽  
10.1038/39587 ◽  
1997 ◽  
Vol 389 (6652) ◽  
pp. 722-725 ◽  
Author(s):  
Arend Sidow ◽  
Monique S. Bulotsky ◽  
Anne W. Kerrebrock ◽  
Roderick T. Bronson ◽  
Mark J. Daly ◽  
...  
Keyword(s):  
Limb Development ◽  
Mouse Limb
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