scholarly journals DNA Methylation at Promoter Regions Regulates the Timing of Gene Activation in Xenopus laevis Embryos

2002 ◽  
Vol 243 (1) ◽  
pp. 155-165 ◽  
Author(s):  
Irina Stancheva ◽  
Osman El-Maarri ◽  
Joern Walter ◽  
Alain Niveleau ◽  
Richard R. Meehan
Keyword(s):  
Dna Methylation ◽  
Xenopus Laevis ◽  
Gene Activation ◽  
Promoter Regions ◽  
Xenopus Laevis Embryos

scholarly journals Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Aziz Taghbalout ◽  
Menghan Du ◽  
Nathaniel Jillette ◽  
Wojciech Rosikiewicz ◽  
Abhijit Rath ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Dna Repair ◽  
Catalytic Domain ◽  
Gene Activation ◽  
Cpg Methylation ◽  
Dna Demethylation ◽  
Promoter Regions ◽  
Repair Pathways

Abstract Here we develop a methylation editing toolbox, Casilio-ME, that enables not only RNA-guided methylcytosine editing by targeting TET1 to genomic sites, but also by co-delivering TET1 and protein factors that couple methylcytosine oxidation to DNA repair activities, and/or promote TET1 to achieve enhanced activation of methylation-silenced genes. Delivery of TET1 activity by Casilio-ME1 robustly alters the CpG methylation landscape of promoter regions and activates methylation-silenced genes. We augment Casilio-ME1 to simultaneously deliver the TET1-catalytic domain and GADD45A (Casilio-ME2) or NEIL2 (Casilio-ME3) to streamline removal of oxidized cytosine intermediates to enhance activation of targeted genes. Using two-in-one effectors or modular effectors, Casilio-ME2 and Casilio-ME3 remarkably boost gene activation and methylcytosine demethylation of targeted loci. We expand the toolbox to enable a stable and expression-inducible system for broader application of the Casilio-ME platforms. This work establishes a platform for editing DNA methylation to enable research investigations interrogating DNA methylomes.

scholarly journals Casilio-ME: Enhanced CRISPR-based DNA demethylation by RNA-guided coupling methylcytosine oxidation and DNA repair pathways

2019 ◽  
Author(s):  
Aziz Taghbalout ◽  
Menghan Du ◽  
Nathaniel Jillette ◽  
Wojciech Rosikiewicz ◽  
Abhijit Rath ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Dna Repair ◽  
Catalytic Domain ◽  
Gene Activation ◽  
Cpg Methylation ◽  
Dna Demethylation ◽  
Promoter Regions ◽  
Transformative Research ◽  
Repair Pathways

ABSTRACTWe have developed a methylation editing toolbox, Casilio-ME, that enables not only RNA-guided methylcytosine editing by targeting TET1 to genomic sites, but also by co-delivering TET1 and protein factors that couple methylcytosine oxidation to DNA repair activities, and/or promote TET1 to achieve enhanced activation of methylation-silenced genes. Delivery of TET1 activity by Casilio-ME1 robustly altered the CpG methylation landscape of promoter regions and activated methylation-silenced genes. We augmented Casilio-ME1 to simultaneously deliver the TET1-catalytic domain and GADD45A (Casilio-ME2) or NEIL2 (Casilio-ME3) to streamline removal of oxidized cytosine intermediates to enhance activation of targeted genes. Using two-in-one effectors or modular effectors, Casilio-ME2 and Casilio-ME3 remarkably boosted gene activation and methylcytosine demethylation of targeted loci. We expanded the toolbox to enable a stable and expression-inducible system for broader application of the Casilio-ME platforms. This work establishes an advanced platform for editing DNA methylation to enable transformative research investigations interrogating DNA methylomes.

scholarly journals Iron nanoparticle bio-interactions evaluated in Xenopus laevis embryos, a model for studying the safety of ingested nanoparticles

2019 ◽  
Vol 14 (2) ◽  
pp. 196-213
Author(s):  
Patrizia Bonfanti ◽  
Anita Colombo ◽  
Melissa Saibene ◽  
Luisa Fiandra ◽  
Ilaria Armenia ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Iron Nanoparticle ◽  
Xenopus Laevis Embryos ◽  
Ingested Nanoparticles

Teratogenic effects of five anticancer drugs on Xenopus laevis embryos

2016 ◽  
Vol 133 ◽  
pp. 90-96 ◽  
Author(s):  
Marina Isidori ◽  
Concetta Piscitelli ◽  
Chiara Russo ◽  
Marie Smutná ◽  
Luděk Bláha
Keyword(s):  
Xenopus Laevis ◽  
Anticancer Drugs ◽  
Teratogenic Effects ◽  
Xenopus Laevis Embryos

Fission yeast tmsl protein abrogates normal development in Xenopus laevis embryos

1995 ◽  
Vol 204 (3) ◽  
pp. 198-202 ◽  
Author(s):  
Peter Wagner ◽  
Michael Hoever ◽  
Katrin Appel ◽  
Walter Kn�chel ◽  
Mathias Montenarh
Keyword(s):  
Xenopus Laevis ◽  
Fission Yeast ◽  
Normal Development ◽  
Xenopus Laevis Embryos
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