scholarly journals Acrosome Reaction in Sperm of the Frog, Xenopus laevis: Its Detection and Induction by Oviductal Pars Recta Secretion

2002 ◽  
Vol 243 (1) ◽  
pp. 55-64 ◽  
Author(s):  
Yasushi Ueda ◽  
Norio Yoshizaki ◽  
Yasuhiro Iwao
Keyword(s):  
Xenopus Laevis ◽  
Acrosome Reaction ◽  
Pars Recta

Enzymatic and envelope-converting activities of pars recta oviductal fluid from Xenopus laevis

1990 ◽  
Vol 138 (1) ◽  
pp. 169-176 ◽  
Author(s):  
Mary-Ann Bakos ◽  
Alexander Kurosky ◽  
Jerry L. Hedrick
Keyword(s):  
Xenopus Laevis ◽  
Pars Recta ◽  
Oviductal Fluid

scholarly journals Analyses of Oviductal Pars Recta-Induced Fertilizability of Coelomic Eggs in Xenopus laevis

1999 ◽  
Vol 210 (2) ◽  
pp. 269-276 ◽  
Author(s):  
Chiaki Katagiri ◽  
Norio Yoshizaki ◽  
Masaharu Kotani ◽  
Hideo Kubo
Keyword(s):  
Xenopus Laevis ◽  
Pars Recta

Enzyme activity in anuran spermatozoa upon induction of the acrosome reaction

Zygote ◽  
2001 ◽  
Vol 9 (4) ◽  
pp. 293-298 ◽  
Author(s):  
V. Infante ◽  
R. Amirante ◽  
M.C. Vaccaro ◽  
M. Wilding ◽  
C. Campanella
Keyword(s):  
Enzyme Activity ◽  
Xenopus Laevis ◽  
Plasminogen Activator ◽  
Protease Inhibitors ◽  
Proteolytic Enzyme ◽  
Acrosome Reaction ◽  
Proteolytic Enzymes ◽  
Dense Material ◽  
Electron Dense Material ◽  
Discoglossus Pictus

At the time of sperm-egg fusion in Discoglossus pictus, a large amount of electron-dense material of an unknown nature is liberated from the sperm. In the present work we studied this material in D. pictus sperm, using an assay utilising strips of autoradiographic film as a gelatin substrate for proteolytic enzymes. Upon treatment with A23187, D. pictus sperm produced a large halo on the gelatin substrate, indicating the presence of enzymes released by the sperm at the time of the acrosome reaction. In contrast, Xenopus laevis sperm did not produce halos upon treatment with A23187. The use of protease inhibitors such as TLCK, leupeptin, chymostatin, SBTI and EACA strongly suggests that the D. pictus whole acrosome contains trypsin and chymotrypsin activity while an SBTI-sensitive activity is absent in a small portion of the acrosome, possibly the anteriormost region. Furthermore, the material released at the acrosome reaction also contains an EACA-inhibited activity, indicating the presence of plasminogen activator. We conclude that D. pictus sperm release proteolytic enzyme(s) that may act at the egg surface at the time of gamete fusion.

Androgen-induced plasticity at a “vocal” neuromuscular synapse

1994 ◽  
Vol 52 ◽  
pp. 32-33
Author(s):  
Darcy B. Kelley ◽  
Martha L. Tobias ◽  
Mark Ellisman
Keyword(s):  
Xenopus Laevis ◽  
Motor Neurons ◽  
Sexual Differentiation ◽  
Molecular Basis ◽  
Neuromuscular Synapse ◽  
Sexually Dimorphic ◽  
Intracellular Protein ◽  
Developmental Program ◽  
Androgen Action ◽  
Clawed Frog

Brain and muscle are sexually differentiated tissues in which masculinization is controlled by the secretion of androgens from the testes. Sensitivity to androgen is conferred by the expression of an intracellular protein, the androgen receptor. A central problem of sexual differentiation is thus to understand the cellular and molecular basis of androgen action. We do not understand how hormone occupancy of a receptor translates into an alteration in the developmental program of the target cell. Our studies on sexual differentiation of brain and muscle in Xenopus laevis are designed to explore the molecular basis of androgen induced sexual differentiation by examining how this hormone controls the masculinization of brain and muscle targets.Our approach to this problem has focused on a highly androgen sensitive, sexually dimorphic neuromuscular system: laryngeal muscles and motor neurons of the clawed frog, Xenopus laevis. We have been studying sex differences at a synapse, the laryngeal neuromuscular junction, which mediates sexually dimorphic vocal behavior in Xenopus laevis frogs.

THE EFFECT OF THE SKIN SECRETION OF XENOPUS LAEVIS ON ITS DERMAL MELANOPHORES

1956 ◽  
Vol 23 (3) ◽  
pp. 265-273 ◽  
Author(s):  
A. C. J. Burgers ◽  
G. J. van Oordt
Keyword(s):  
Xenopus Laevis ◽  
Skin Secretion
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