scholarly journals Induction of Early Transcription in One-Cell Mouse Embryos by Microinjection of the Nonhistone Chromosomal Protein HMG-I

2000 ◽  
Vol 221 (2) ◽  
pp. 337-354 ◽  
Author(s):  
Nathalie Beaujean ◽  
Christine Bouniol-Baly ◽  
Caroline Monod ◽  
Karima Kissa ◽  
Denis Jullien ◽  
...  
Keyword(s):  
Mouse Embryos ◽  
Chromosomal Protein ◽  
Early Transcription ◽  
Nonhistone Chromosomal Protein

Facultative heterochromatization in parahaploid male mealybugs: involvement of a heterochromatin-associated protein

Development ◽  
2001 ◽  
Vol 128 (19) ◽  
pp. 3809-3817 ◽  
Author(s):  
Silvia Bongiorni ◽  
Milena Mazzuoli ◽  
Stefania Masci ◽  
Giorgio Prantera
Keyword(s):  
Monoclonal Antibody ◽  
Drosophila Melanogaster ◽  
Constitutive Heterochromatin ◽  
Planococcus Citri ◽  
Chromosomal Protein ◽  
Paternal Chromosome ◽  
Localization Pattern ◽  
Males And Females ◽  
Male Specific ◽  
Nonhistone Chromosomal Protein

The behavior of chromosomes during development of the mealybug Planococcus citri provides one of the most dramatic examples of facultative heterochromatization. In male embryos, the entire haploid paternal chromosome set becomes heterochromatic at mid-cleavage. Male mealybugs are thus functionally haploid, owing to heterochromatization (parahaploidy). To understand the mechanisms underlying facultative heterochromatization in male mealybugs, we have investigated the possible involvement of an HP-1-like protein in this process. HP-1 is a conserved, nonhistone chromosomal protein with a proposed role in heterochromatinization in other species. It was first identified in Drosophila melanogaster as a protein enriched in the constitutive heterochromatin of polytene chromosome. Using a monoclonal antibody raised against the Drosophila HP-1 in immunoblot and immunocytological experiments, we provide evidence for the presence of an HP-1-like in Planococcus citri males and females. In males, the HP-1-like protein is preferentially associated with the male-specific heterochromatin. In the developing male embryos, its appearance precedes the onset of heterochromatization. In females, the HP-1-like protein displays a scattered but reproducible localization pattern along chromosomes. The results indicate a role for an HP-1-like protein in the facultative heterochromatization process.

Structure of DNA Complexes with Nonhistone Chromosomal Protein HMGB1 in the Presence of Manganese Ions

2004 ◽  
Vol 38 (6) ◽  
pp. 891-898 ◽  
Author(s):  
A. M. Polyanichko ◽  
E. V. Chikhirzhina ◽  
E. I. Kostyleva ◽  
V. I. Vorob'ev
Keyword(s):  
Chromosomal Protein ◽  
Manganese Ions ◽  
Dna Complexes ◽  
Nonhistone Chromosomal Protein

scholarly journals Interaction between Chromosomal Protein HMGB1 and DNA Studied by DNA-Melting Analysis

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Elena V. Chikhirzhina ◽  
Starkova J. Tatiana ◽  
Alexander M. Polyanichko
Keyword(s):  
Circular Dichroism ◽  
Thermal Denaturation ◽  
Circular Dichroism Spectroscopy ◽  
Dna Melting ◽  
Chromosomal Protein ◽  
Melting Temperatures ◽  
Melting Analysis ◽  
Circular Dichroïsm ◽  
Nonhistone Chromosomal Protein ◽  
Biphasic Process

Interaction of HMGB1 nonhistone chromosomal protein with DNA was studied using circular dichroism spectroscopy and thermal denaturation of DNA. Melting DNA in the complex was shown to be a biphasic process. The characteristic melting temperatures of unbound DNA and the DNA bound to HMGB1 in 0.25 mM EDTA solutions were found to beTmI=44.0±0.5°C andTmII=62.0±1°C, respectively. It was shown that the binding of the HMGB1 molecule affects the melting of the DNA region approximately 30 b.p. long.

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