scholarly journals Plasticity of Retrovirus-Labelled Myotubes in the Newt Limb Regeneration Blastema

2000 ◽  
Vol 218 (2) ◽  
pp. 125-136 ◽  
Author(s):  
Anoop Kumar ◽  
Cristiana P. Velloso ◽  
Yutaka Imokawa ◽  
Jeremy P. Brockes
Keyword(s):  
Limb Regeneration ◽  
Regeneration Blastema ◽  
Newt Limb

Limb regeneration blastema cells in the adult newt, Notophthalmus viridescens, give aberrant response to colchicine

1983 ◽  
Vol 61 (3) ◽  
pp. 553-559 ◽  
Author(s):  
Brenda L. Coomber ◽  
Karin J. Davidson ◽  
Steven R. Scadding
Keyword(s):  
Body Weight ◽  
Mitotic Index ◽  
Treatment Time ◽  
Limb Regeneration ◽  
Duodenal Mucosa ◽  
Index Number ◽  
High Doses ◽  
Mitotic Figures ◽  
Regeneration Blastema ◽  
Newt Limb

This study was designed to investigate the effect of colchicine on the mitotic index (number of mitotic figures per 1000 cells) in the newt limb regeneration blastema. Colchicine doses from 0.1 to 1000 μg per gram body weight and treatment durations from 2 to 72 h were used to determine the dose of colchicine and treatment period resulting in optimum metaphase arrest. A preliminary experiment demonstrated that there was no circadian rhythm of mitosis in the newt limb regeneration blastema. A dose of 5 μg colchicine per gram body weight was found to give the maximum mitotic index; both higher and lower doses had less effect. Very high doses of colchicine resulted in a mitotic index the same as that of the controls. The mitotic index increased with increasing treatment time up to 36 h, after which there was no further increase. In other newt tissues such as duodenal mucosa and liver, a more typical dose–response relationship was observed in that increased colchicine dose led to increased mitotic indices up to the level of lethal doses of colchicine.

scholarly journals Normal newt limb regeneration requires matrix metalloproteinase function

2005 ◽  
Vol 279 (1) ◽  
pp. 86-98 ◽  
Author(s):  
Vladimir Vinarsky ◽  
Donald L. Atkinson ◽  
Tamara J. Stevenson ◽  
Mark T. Keating ◽  
Shannon J. Odelberg
Keyword(s):  
Matrix Metalloproteinase ◽  
Limb Regeneration ◽  
Newt Limb

scholarly journals The Role of the Prod1 Membrane Anchor in Newt Limb Regeneration

2016 ◽  
Vol 129 (1) ◽  
pp. 276-280
Author(s):  
Kaoru Nomura ◽  
Yasushi Tanimoto ◽  
Fumio Hayashi ◽  
Erisa Harada ◽  
Xiao-Yuan Shan ◽  
...  
Keyword(s):  
Limb Regeneration ◽  
Membrane Anchor ◽  
Newt Limb

Symmetrical vascularization patterns in normal and retinoic acid treated limb regenerates of the axolotl, Ambystoma mexicanum

1998 ◽  
Vol 76 (9) ◽  
pp. 1795-1796 ◽  
Author(s):  
Steven R Scadding ◽  
Andrew Burns
Keyword(s):  
Retinoic Acid ◽  
Pattern Formation ◽  
Vascular System ◽  
Limb Regeneration ◽  
Ambystoma Mexicanum ◽  
Limb Pattern ◽  
Regeneration Blastema

The purpose of this investigation was to determine whether there were any asymmetries in the vascularization of the limb-regeneration blastema in the axolotl, Ambystoma mexicanum, that might be related to pattern formation, and to determine if retinoic acid could modify the vascular patterns of the blastema. We used acrylic casts of the vascular system of the limbs to assess the pattern of vascularization. We observed a very regular symmetrical arrangement of capillaries in the limb-regeneration blastema that did not appear to be modified by doses of retinoic acid sufficient to modify the limb pattern.

A monoclonal antibody stains myogenic cells in regenerating newt muscle

Development ◽  
1987 ◽  
Vol 101 (2) ◽  
pp. 267-277 ◽  
Author(s):  
K.J. Griffin ◽  
D.M. Fekete ◽  
B.M. Carlson
Keyword(s):  
Monoclonal Antibody ◽  
Muscle Regeneration ◽  
Tissue Repair ◽  
Limb Regeneration ◽  
Alternative Interpretation ◽  
Muscle Fibres ◽  
Muscle Repair ◽  
Contralateral Limb ◽  
Minced Muscle ◽  
Regeneration Blastema

Monoclonal antibodies have been used to study minced muscle regeneration in the adult newt, Notophthalmus viridescens. The contralateral limb was amputated and the immunostaining patterns in the regenerating blastema were compared with the minced tissue in sectioned material. Staining with a myofibre-specific antibody, called 12/101 (Kintner & Brockes, 1984), showed that myofibre degeneration was complete by 8–10 days after mincing, with myogenesis commencing 2 days later. Another monoclonal antibody, called 22/18, previously shown to label a subset of cells in the regeneration blastema of the newt (Kintner & Brockes, 1984, 1985), was found also to recognize a population of cells in regenerating minced muscle. At 6 days after mincing, the number of 22/18-positive (22/18+) cells was low but by days 12–16, during the period of myogenesis, their number had increased to become a major population within the minced tissue. A small number of the 22/18+ cells could be double labelled with 12/101 at this time. Prior to this, there was a phase in which 12/101 staining had disappeared from the mince. Cells immunoreactive with both antibodies after this phase confirm that at least some of the 22/18+ cells are myogenic. The number of 22/18+ cells decreased as muscle repair and maturation progressed. These results show that 22/18 is not specifically associated with blastemal cells but is a more general marker for regenerating systems in the newt. They further suggest an alternative interpretation of the double-labelled cells used by Kintner & Brockes (1984) as evidence for myofibre dedifferentiation in limb regeneration. Instead, we propose that such cells represent new myogenesis occurring by tissue repair of locally damaged muscle fibres.

Analysis of Hox-4.5 and Hox-3.6 expression during newt limb regeneration: differential regulation of paralogous Hox genes suggest different roles for members of different Hox clusters

Development ◽  
1993 ◽  
Vol 117 (4) ◽  
pp. 1397-1407 ◽  
Author(s):  
H.G. Simon ◽  
C.J. Tabin
Keyword(s):  
Hox Genes ◽  
Limb Regeneration ◽  
Differential Regulation ◽  
Epithelial Tissue ◽  
Cdna Clones ◽  
Rnase Protection ◽  
Adult Kidney ◽  
Early Expression ◽  
Hox Clusters ◽  
Newt Limb

Adult urodele amphibians can regenerate their limbs and tail. Based on their roles in other developing systems, Hox genes are strong candidates for genes that play a role in regulating pattern formation during regeneration. There are four homologous clusters of Hox genes in vertebrate genomes. We isolated cDNA clones of two newt homeobox genes from homologous positions within two Hox clusters; Hox-4.5 and Hox-3.6. We used RNase protection on nonamputated (normal) and regenerating newt appendages and tissue to compare their transcriptional patterns. Both genes show increased expression upon amputation with similar kinetics. Hox-4.5 and Hox-3.6 transcription is limited to the mesenchymal cells in the regenerates and is not found in the epithelial tissue. In addition to regenerating appendages, both genes are transcriptionally active in adult kidney of the newt. Striking differences were found in the regulation of Hox-4.5 and Hox-3.6 when they were compared in unamputated limbs and in regenerating forelimbs versus regenerating hindlimbs. Hox-4.5 is expressed in the blastema of regenerating fore- and hindlimbs, but Hox-4.5 transcripts are not detectable in normal limbs. In contrast, Hox-3.6 transcripts are found exclusively in posterior appendages, but are present in normal as well as regenerating hindlimbs and tails. Hox-4.5 is also expressed at a higher level in proximal (mid-humerus) regenerates than in distal ones (mid-radius). When we proximalized the positional memory of a distal blastema with retinoic acid, we find that the early expression level of Hox-4.5 is also proximalized. When the expression of these genes is compared to the expression of two previously reported newt Hox genes, a consistent pattern emerges, which can be interpreted in terms of differential roles for the different Hox clusters in determining regenerative limb morphology.

Sign in / Sign up

Export Citation Format

Share Document