scholarly journals Spatial and Temporal Changes in Myosin Heavy Chain Gene Expression in Skeletal Muscle Development

1999 ◽  
Vol 216 (1) ◽  
pp. 312-326 ◽  
Author(s):  
Brian D. Lu ◽  
David L. Allen ◽  
Leslie A. Leinwand ◽  
Gary E. Lyons
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Muscle Development ◽  
Chain Gene ◽  
Skeletal Muscle Development ◽  
Heavy Chain Gene ◽  
Myosin Heavy Chain Gene ◽  
Spatial And Temporal Changes

scholarly journals Evidence of MyomiR network regulation of β-myosin heavy chain gene expression during skeletal muscle atrophy

2009 ◽  
Vol 39 (3) ◽  
pp. 219-226 ◽  
Author(s):  
John J. McCarthy ◽  
Karyn A. Esser ◽  
Charlotte A. Peterson ◽  
Esther E. Dupont-Versteegden
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Muscle Atrophy ◽  
Soleus Muscle ◽  
Heavy Chain ◽  
Hindlimb Suspension ◽  
Chain Gene ◽  
Heavy Chain Gene ◽  
Myosin Heavy Chain Gene

There is a growing recognition that noncoding RNAs (ncRNA) play an important role in the regulation of gene expression. A class of small (19–22 nt) ncRNAs, known as microRNAs (miRs), have received a great deal of attention lately because of their ability to repress gene expression through a unique posttranscriptional 3′-untranslated region (UTR) mechanism. The objectives of the current study were to identify miRs expressed in the rat soleus muscle and determine if their expression was changed in response to hindlimb suspension. Comprehensive profiling revealed 151 miRs were expressed in the soleus muscle and expression of 18 miRs were significantly ( P < 0.01) changed after 2 and/or 7 days of hindlimb suspension. The significant decrease (16%) in expression of muscle-specific miR-499 in response to hindlimb suspension was confirmed by RT-PCR and suggested activation of the recently proposed miR encoded by myosin gene (MyomiR) network during atrophy. Further analysis of soleus muscle subjected to hindlimb suspension for 28 days provided evidence consistent with MyomiR network repression of β-myosin heavy chain gene (β-MHC) expression. The significant downregulation of network components miR-499 and miR-208b by 40 and 60%, respectively, was associated with increased expression of Sox6 (2.2-fold) and Purβ (23%), predicted target genes of miR-499 and known repressors of β-MHC expression. A Sox6 3′-UTR reporter gene confirmed Sox6 is a target gene of miR-499. These results further expand the role of miRs in adult skeletal muscle and are consistent with a model in which the MyomiR network regulates slow myosin expression during muscle atrophy.

The Influence of Dietary Salt and Plasma Renin Activity on Myosin Heavy Chain Gene Expression in Rat Hearts

1993 ◽  
Vol 6 (7 Pt 1) ◽  
pp. 579-585 ◽  
Author(s):  
P. Buttrick ◽  
M. Kaplan ◽  
M. J. F. Camargo ◽  
G. deSimone ◽  
J. H. Laragh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Plasma Renin Activity ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Plasma Renin ◽  
Chain Gene ◽  
Heavy Chain Gene ◽  
Dietary Salt ◽  
Myosin Heavy Chain Gene ◽  
Rat Hearts
Sign in / Sign up

Export Citation Format

Share Document