scholarly journals Expression Cloning of an Ascidian Syndecan Suggests Its Role in Embryonic Cell Adhesion and Morphogenesis

1999 ◽  
Vol 211 (2) ◽  
pp. 198-207 ◽  
Author(s):  
Yutaka Satou ◽  
Shota Chiba ◽  
Nori Satoh
Keyword(s):  
Cell Adhesion ◽  
Embryonic Cell ◽  
Expression Cloning

Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytes

Cell ◽  
1989 ◽  
Vol 59 (6) ◽  
pp. 1203-1211 ◽  
Author(s):  
Laurelee Osborn ◽  
Catherine Hession ◽  
Richard Tizard ◽  
Cornelia Vassallo ◽  
Stefan Luhowskyj ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Expression Cloning ◽  
Vascular Cell Adhesion ◽  
Vascular Cell ◽  
Vascular Cell Adhesion Molecule ◽  
Cell Adhesion Molecule 1

scholarly journals The human E48 antigen, highly homologous to the murine Ly-6 antigen ThB, is a GPI-anchored molecule apparently involved in keratinocyte cell-cell adhesion.

1995 ◽  
Vol 129 (6) ◽  
pp. 1677-1689 ◽  
Author(s):  
R H Brakenhoff ◽  
M Gerretsen ◽  
E M Knippels ◽  
M van Dijk ◽  
H van Essen ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Core Protein ◽  
Sequence Similarity ◽  
Single Copy ◽  
Chromosome 8 ◽  
Expression Cloning ◽  
Target Antigen ◽  
Reading Frame ◽  
Antigen A ◽  
Cell Cell

The E48 antigen, a putative human homologue of the 20-kD protein present in desmosomal preparations of bovine muzzle, and formerly called desmoglein III (dg4), is a promising target antigen for antibody-based therapy of squamous cell carcinoma in man. To anticipate the effect of high antibody dose treatment, and to evaluate the possible biological involvement of the antigen in carcinogenesis, we set out to molecularly characterize the antigen. A cDNA clone encoding the E48 antigen was isolated by expression cloning in COS cells. Sequence analysis revealed that the clone contained an open reading frame of 128 amino acids, encoding a core protein of 13,286 kD. Database searching showed that the E48 antigen has a high level of sequence similarity with the mouse ThB antigen, a member of the Ly-6 antigen family. Phosphatidylinositol-specific (PI-specific) phospholipase-C treatment indicated that the E48 antigen is glycosylphosphatidylinositol-anchored (GPI-anchored) to the plasma membrane. The gene encoding the E48 antigen is a single copy gene, located on human chromosome 8 in the 8q24-qter region. The expression of the gene is confined to keratinocytes and squamous tumor cells. The putative mouse homologue, the ThB antigen, originally identified as an antigen on cells of the lymphocyte lineage, was shown to be highly expressed in squamous mouse epithelia. Moreover, the ThB expression level is in keratinocytes, in contrast to that in lymphocytes, not mouse strain related. Transfection of mouse SV40-polyoma transformed mouse NIH/3T3 cells with the E48 cDNA confirmed that the antigen is likely to be involved in cell-cell adhesion.

Regulation of Xenopus embryonic cell adhesion by the small GTPase, rac

2002 ◽  
Vol 298 (3) ◽  
pp. 364-370 ◽  
Author(s):  
Mark D Hens ◽  
Ivana Nikolic ◽  
Caron M Woolcock
Keyword(s):  
Cell Adhesion ◽  
Embryonic Cell ◽  
Small Gtpase ◽  
Gtpase Rac

A kinetic study of embryonic cell adhesion

1975 ◽  
Vol 43 (1) ◽  
pp. 109-122 ◽  
Author(s):  
David R. McClay ◽  
Susan R. Baker
Keyword(s):  
Cell Adhesion ◽  
Kinetic Study ◽  
Embryonic Cell
Sign in / Sign up

Export Citation Format

Share Document