scholarly journals Mechanical Signals in Plant Development: A New Method for Single Cell Studies

1997 ◽  
Vol 181 (2) ◽  
pp. 246-256 ◽  
Author(s):  
Timothy M. Lynch ◽  
Philip M. Lintilhac
2019 ◽  
Vol 11 (7) ◽  
pp. 3443-3452
Author(s):  
C. J. Dalmas Neto ◽  
E. B. Sydney ◽  
E. S. Candeo ◽  
E. B. S. de Souza ◽  
D. Camargo ◽  
...  

2019 ◽  
Vol 70 (14) ◽  
pp. 3507-3519 ◽  
Author(s):  
Benoit Landrein ◽  
Gwyneth Ingram

Abstract As multicellular organisms, plants acquire characteristic shapes through a complex set of biological processes known as morphogenesis. Biochemical signalling underlies much of development, as it allows cells to acquire specific identities based on their position within tissues and organs. However, as growing physical structures, plants, and their constituent cells, also experience internal and external physical forces that can be perceived and can influence key processes such as growth, polarity, and gene expression. This process, which adds another layer of control to growth and development, has important implications for plant morphogenesis. This review provides an overview of recent research into the role of mechanical signals in plant development and aims to show how mechanical signalling can be used, in concert with biochemical signals, as a cue allowing cells and tissues to coordinate their behaviour and to add robustness to developmental processes.


1995 ◽  
Vol 350 (1331) ◽  
pp. 83-86 ◽  

The involvement of mechanical signals (tension and compression) in the determination of the form of living organisms has been speculated upon for many years. These mechanical signals (both environmental and those generated within the plant itself) have significant effects on plant development and thus morphology. Plants respond to externally applied mechanical signals (touch and wind) by an immediate elevation of cytosolic calcium concentration ([Ca 2+ ] eyt ) in stimulated cells. This response requires the movement of plant tissues to cause tension and compression. Some of the more longer-term responses to mechanical signals, e.g. TCH gene expression and reduction in hypocotyl growth, show a calcium-dependency. It seems likely, therefore, that the effects of mechanical signals on plant development are mediated by the second messenger, calcium. This raises the exciting possibility that this simple ion plays a central role in the determination of plant form itself.


1989 ◽  
Vol 44 (9-10) ◽  
pp. 845-848 ◽  
Author(s):  
Jerzy J. Zieliński ◽  
Piotr Marszałek ◽  
Magdalena Fikus

A new method and a new type of measuring-microchamber for the investigation of cellular dielectrophoresis is presented. The method is based on the observation of the trajectory of a single cell in gravitational and electric fields being crossed and orientated perpendicularly to the observation direction. By means of this method the whole dielectrophoretic spectrum ranging from negative to positive dielectrophoresis may be easily and quickly obtained. The experiments carried out on different cell types showed that the dielectrophoretic spectra, as well as the dependence of the critical frequency upon medium conductivity and cell size agree well with the predictions of a new model of the dielectrophoretic mechanism proposed by Sauer.


2018 ◽  
Vol 5 (5) ◽  
pp. 969-978 ◽  
Author(s):  
Sijie Chen ◽  
Jianzhao Liu ◽  
Shouxiang Zhang ◽  
Engui Zhao ◽  
Chris Yee Yung Yu ◽  
...  

A new method using a single cell-permeable biochromic fluorescent dye to differentiate, quantify and image both live and dead cells is reported.


2020 ◽  
Vol 114 (3) ◽  
pp. e524
Author(s):  
Fabiana B. Kohlrausch ◽  
Fang Wang ◽  
Wilson McKerrow ◽  
David Fenyo ◽  
Jef D. Boeke ◽  
...  

2020 ◽  
Vol 210 ◽  
pp. 03006
Author(s):  
Ermat Kubeev ◽  
Viktor Smelilk

Modern technologies for applying artificial shells are based on the principles of rolling or granulating with subsequent drying. This technology does not allow to get a gas - permeable porous shell, which often leads to the death of seeds during the formation of the shell, storage and transportation, and slow growth at the early stages of plant development. The aim of the research is to develop a new method and device for pre-sowing treatment of agricultural seeds, which can increase their germination, germination energy and ensure crop growth. A distinctive feature of the new method is that the shell is formed not by rolling, but by molding around the seed, followed by drying in a soft mode. This will form a porous, gas-permeable structure around the seed. Based on the mathematical description of the processes occurring during the introduction of seeds into the nutrient medium, the design calculations and calculations made a laboratory stand for the study of the seeds coating with a protective and nutritious shell, which consists of a mixing, sealing and seeding nodes. A series of experiments was conducted to study the operation of the stand in various modes. Optimal operating modes are identified.


2021 ◽  
Vol 35 (1) ◽  
Author(s):  
Jalila Benjelloun ◽  
Sarah Bouzroud ◽  
Zine El Abidine Triqui ◽  
Qamar Lahlimi Alami ◽  
Rajaa Layachi ◽  
...  

The broad objective of this research is to study the effect of warm stratification on Cycas revoluta zygotic embryos length, seed germination and plant development. Four warm treatments were applied to seeds. Our results showed that seeds storage at room temperature or 30°C improved significantly zygotic embryos length. Moreover, time of germination was significantly reduced with the warm stratification. The highest percentage of germination was obtained with seeds warm treated at 30°C for 2 months while only 25% of seeds were able to germinate in the control. Regarding seedlings development, our results demonstrated that warm stratification did not affect plant development. No significant differences have been recorded in all the evaluated parameters except for root length. Taken together, these results underlined the beneficial effect of warm stratification on Cycas revoluta seed germination and plant development and proposed a new method to improve seed germination of Cycas revoluta.


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