scholarly journals Biphasic Activation of Fyn Kinase upon Fertilization of the Sea Urchin Egg

1996 ◽  
Vol 174 (2) ◽  
pp. 281-287 ◽  
Author(s):  
William H. Kinsey
Keyword(s):  
Sea Urchin ◽  
Fyn Kinase ◽  
Sea Urchin Egg

scholarly journals Different Ca2+-releasing abilities of sperm extracts compared with tissue extracts and phospholipase C isoforms in sea urchin egg homogenate and mouse eggs

2000 ◽  
Vol 346 (3) ◽  
pp. 743-749 ◽  
Author(s):  
Keith T. JONES ◽  
Miho MATSUDA ◽  
John PARRINGTON ◽  
Matilda KATAN ◽  
Karl SWANN
Keyword(s):  
Phospholipase C ◽  
Sea Urchin ◽  
Tissue Extracts ◽  
Sea Urchin Egg ◽  
Boar Sperm ◽  
Specific Activities ◽  
Mammalian Eggs ◽  
Mouse Eggs

A soluble phospholipase C (PLC) from boar sperm generates InsP3 and hence causes Ca2+ release when added to sea urchin egg homogenate. This PLC activity is associated with the ability of sperm extracts to cause Ca2+ oscillations in mammalian eggs following fractionation. A sperm PLC may, therefore, be responsible for causing the observed Ca2+ oscillations at fertilization. In the present study we have further characterized this boar sperm PLC activity using sea urchin egg homogenate. Consistent with a sperm PLC acting on egg PtdIns(4,5)P2, the ability of sperm extracts to release Ca2+ was blocked by preincubation with the PLC inhibitor U73122 or by the addition of neomycin to the homogenate. The Ca2+-releasing activity was also detectable in sperm from other species and in whole testis extracts. However, activity was not observed in extracts from other tissues. Moreover recombinant PLCβ1, -γ1, -γ2, -∆1, all of which had higher specific activities than boar sperm extracts, were not able to release Ca2+ in the sea urchin egg homogenate. In addition these PLCs were not able to cause Ca2+ oscillations following microinjection into mouse eggs. These results imply that the sperm PLC possesses distinct properties that allow it to hydrolyse PtdIns(4,5)P2 in eggs.

ACID-SOLUBLE NUCLEOTIDES IN THE SEA URCHIN EGG

Embryologia ◽  
1966 ◽  
Vol 9 (3) ◽  
pp. 170-183 ◽  
Author(s):  
TOMIO YANAGISAWA ◽  
NAOHIDE ISONO
Keyword(s):  
Sea Urchin ◽  
Sea Urchin Egg

Re-fertilization of the sea urchin egg

1954 ◽  
Vol 6 (2) ◽  
pp. 491-496 ◽  
Author(s):  
B. Hagström ◽  
Britt Hagström
Keyword(s):  
Sea Urchin ◽  
Sea Urchin Egg

Characterization of the sea urchin egg receptor for sperm

1996 ◽  
Vol 24 (1) ◽  
pp. 100S-100S
Author(s):  
Kay Ohlendieck ◽  
William J. Lennarz
Keyword(s):  
Sea Urchin ◽  
Sea Urchin Egg

THE SEA URCHIN EGG AS A TESTING OBJECT IN TOXICOLOGY

2009 ◽  
Vol 32 ◽  
pp. 1-49 ◽  
Author(s):  
Berndt E. Hagström ◽  
Sunniva Lönning
Keyword(s):  
Sea Urchin ◽  
Sea Urchin Egg
Sign in / Sign up

Export Citation Format

Share Document