scholarly journals Immunolocalization of the Heterotrimeric Kinesin-Related Protein KRP(85/95) in the Mitotic Apparatus of Sea Urchin Embryos

1995 ◽  
Vol 171 (1) ◽  
pp. 182-194 ◽  
Author(s):  
John H. Henson ◽  
Douglas G. Cole ◽  
Mark Terasaki ◽  
Dana Rashid ◽  
Jonathan M. Scholey
Keyword(s):  
Sea Urchin ◽  
Related Protein ◽  
Mitotic Apparatus ◽  
Sea Urchin Embryos

scholarly journals Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection.

1993 ◽  
Vol 123 (3) ◽  
pp. 681-689 ◽  
Author(s):  
B D Wright ◽  
M Terasaki ◽  
J M Scholey
Keyword(s):  
Cell Division ◽  
Sea Urchin ◽  
Heavy Chain ◽  
Mammalian Cells ◽  
Embryonic Cell ◽  
Mitotic Apparatus ◽  
Specific Antibodies ◽  
Sea Urchin Embryos ◽  
Mitotic Figures

Previous studies suggest that kinesin heavy chain (KHC) is associated with ER-derived membranes that accumulate in the mitotic apparatus in cells of early sea urchin embryos (Wright, B. D., J. H. Henson, K. P. Wedaman, P. J. Willy, J. N. Morand, and J. M. Scholey. 1991. J. Cell Biol. 113:817-833). Here, we report that the microinjection of KHC-specific antibodies into these cells has no effect on mitosis or ER membrane organization, even though one such antibody, SUK4, blocks kinesin-driven motility in vitro and in mammalian cells. Microinjected SUK4 was localized to early mitotic figures, suggesting that it is able to access kinesin in spindles. In contrast to KHC-specific antibodies, two antibodies that react with kinesin-like proteins (KLPs), namely CHO1 and HD, disrupted mitosis and prevented subsequent cell division. CHO1 is thought to exert this effect by blocking the activity of a 110-kD KLP. The relevant target of HD, which was raised against the KHC motor domain, is unknown; HD may disrupt mitosis by interfering with an essential spindle KLP but not with KHC itself, as preabsorption of HD with KHC did not alter its ability to block mitosis. These data indicate that some KLPs have essential mitotic functions in early sea urchin embryos but KHC itself does not.

scholarly journals SOME STRUCTURAL AND FUNCTIONAL ASPECTS OF THE MITOTIC APPARATUS IN SEA URCHIN EMBRYOS

1962 ◽  
Vol 14 (3) ◽  
pp. 475-487 ◽  
Author(s):  
Patricia Harris
Keyword(s):  
Sea Urchin ◽  
Salt Concentration ◽  
Sea Water ◽  
Divalent Cations ◽  
Cell Stage ◽  
Mitotic Apparatus ◽  
Central Spindle ◽  
Sea Urchin Embryos ◽  
Cilia Formation ◽  
Dividing Cells

The mitotic figures in dividing cells of sea urchin embryos, from first division to the onset of cilia formation, were studied with regard to the filament system and its relation to kinetochores, chromosomes, and poles, as well as to fixation conditions which would best preserve these structures. With regard to fixation, variations in the salt concentration and pH of the fixative indicated that an extraction effect on the chromosomes noted in earlier work was probably due to a combination of neutral pH and salt concentration equivalent to sea water. The presence of the 15 mµ filaments depended on the presence of either of two stabilizing conditions: pH 6.1 or presence of the salts of sea water, presumably the divalent cations of Ca and Mg. Kinetochores and centrioles were unaffected by the fixative variations. The 15 mµ filaments, reported earlier in the central spindle, are also found in great numbers in the asters of early cleavage divisions. However, with successive divisions and reduction in cell size, the aster disappears at about the 32 to 64 cell stage, and the 15 mµ filaments are entirely associated with the central spindle. This disappearance of the aster suggests that it may be, in fact, merely a specialization of large cells for cytokinesis.

Unusual pattern of accumulation of mRNA encoding EGF-related protein in sea urchin embryos

Science ◽  
1989 ◽  
Vol 246 (4931) ◽  
pp. 806-808 ◽  
Author(s):  
Q Yang ◽  
L. Angerer ◽  
R. Angerer
Keyword(s):  
Sea Urchin ◽  
Related Protein ◽  
Unusual Pattern ◽  
Sea Urchin Embryos
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