Regulated Expression of Wnt Family Members during Neuroectodermal Differentiation of P19 Embryonal Carcinoma Cells: Overexpression of Wnt-1 Perturbs Normal Differentiation-Specific Properties

1994 ◽  
Vol 166 (1) ◽  
pp. 300-310 ◽  
Author(s):  
Beverly D. Smolich ◽  
Jackie Papkoff
Keyword(s):  
Embryonal Carcinoma ◽  
Family Members ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Regulated Expression ◽  
P19 Embryonal Carcinoma Cells ◽  
Normal Differentiation

scholarly journals Synthesis and processing of small B2 transcripts in mouse embryonal carcinoma cells.

1990 ◽  
Vol 10 (8) ◽  
pp. 4058-4067 ◽  
Author(s):  
T S Bladon ◽  
C J Frégeau ◽  
M W McBurney
Keyword(s):  
Embryonal Carcinoma ◽  
Consensus Sequence ◽  
Rna Polymerase Iii ◽  
Cell Types ◽  
Nucleocytoplasmic Transport ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
B2 Rna ◽  
P19 Embryonal Carcinoma Cells ◽  
Rna Levels

B2 genes are short repeated sequences which are transcribed by RNA polymerase III. Abundant transcripts accumulate in embryonic and transformed cells, but transcripts are rare or absent from normal differentiated cell types. During retinoic acid-induced differentiation of P19 embryonal carcinoma cells, an early transient increase in B2 RNA levels is followed by a rapid drop in expression. The marked changes in B2 RNA levels are most likely due to transcriptional modulation since B2 RNA stabilities are unaffected by differentiation. At least four short-lived B2 RNAs with apparent lengths of 150, 180, 240, and 500 nucleotides were characterized. The two larger RNAs are polyadenylated and are more stable in cells. A cDNA of a B2 gene was isolated which was over 99% identical to the consensus sequence. This B2 cDNA can be transcribed in human cells and yields at least two distinct transcripts. We propose a model for B2 RNA metabolism which describes transcription, posttranscriptional modification and processing, and nucleocytoplasmic transport.

scholarly journals Genome-wide demethylation during neural differentiation of P19 embryonal carcinoma cells

2008 ◽  
Vol 53 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Izuho Hatada ◽  
Sumiyo Morita ◽  
Mika Kimura ◽  
Takuro Horii ◽  
Riu Yamashita ◽  
...  
Keyword(s):  
Neural Differentiation ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Genome Wide ◽  
P19 Embryonal Carcinoma Cells

scholarly journals Regulated expression of an introduced MHC H–2Kbm1 gene in murine embryonal carcinoma cells

Nature ◽  
1984 ◽  
Vol 310 (5976) ◽  
pp. 415-418 ◽  
Author(s):  
Arnon Rosenthal ◽  
Stephanie Wright ◽  
Howard Cedar ◽  
Richard Flavell ◽  
Frank Grosveld
Keyword(s):  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Regulated Expression

Neurogenesis Using P19 Embryonal Carcinoma Cells

10.3791/58225 ◽  
2019 ◽  
Author(s):  
Paweł Leszczyński ◽  
Magdalena Śmiech ◽  
Aamir S. Teeli ◽  
Aleksandra Zołocińska ◽  
Anna Słysz ◽  
...  
Keyword(s):  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
P19 Embryonal Carcinoma Cells
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