Changes in Expression of Nonmuscle Myosin Heavy Chain Isoforms during Muscle and Nonmuscle Tissue Development

1993 ◽  
Vol 157 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Noriko Murakami ◽  
Ekkhart Trenkner ◽  
Marshall Elzinga
Keyword(s):  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Myosin Heavy Chain Isoforms ◽  
Nonmuscle Myosin ◽  
Tissue Development

scholarly journals Xenopus nonmuscle myosin heavy chain isoforms have different subcellular localizations and enzymatic activities.

1996 ◽  
Vol 134 (3) ◽  
pp. 675-687 ◽  
Author(s):  
C A Kelley ◽  
J R Sellers ◽  
D L Gard ◽  
D Bui ◽  
R S Adelstein ◽  
...  
Keyword(s):  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Myosin Heavy Chain Isoforms ◽  
Enzymatic Activities ◽  
Cell Cortex ◽  
Cell Periphery ◽  
Nonmuscle Myosin ◽  
In Vitro Motility Assay ◽  
Interphase Cells

There are two isoforms of the vertebrate nonmuscle myosin heavy chain, MHC-A and MHC-B, that are encoded by two separate genes. We compared the enzymatic activities as well as the subcellular localizations of these isoforms in Xenopus cells. MHC-A and MHC-B were purified from cells by immunoprecipitation with isoform-specific peptide antibodies followed by elution with their cognate peptides. Using an in vitro motility assay, we found that the velocity of movement of actin filaments by MHC-A was 3.3-fold faster than that by MHC-B. Likewise, the Vmax of the actin-activated Mg(2+)-ATPase activity of MHC-A was 2.6-fold greater than that of MHC-B. Immunofluorescence microscopy demonstrated distinct localizations for MHC-A and MHC-B. In interphase cells, MHC-B was present in the cell cortex and diffusely arranged in the cytoplasm. In highly polarized, rapidly migrating interphase cells, the lamellipodium was dramatically enriched for MHC-B suggesting a possible involvement of MHC-B based contractions in leading edge extension and/or retraction. In contrast, MHC-A was absent from the cell periphery and was arranged in a fibrillar staining pattern in the cytoplasm. The two myosin heavy chain isoforms also had distinct localizations throughout mitosis. During prophase, the MHC-B redistributed to the nuclear membrane, and then resumed its interphase localization by metaphase. MHC-A, while diffuse within the cytoplasm at all stages of mitosis, also localized to the mitotic spindle in two different cultured cell lines as well as in Xenopus blastomeres. During telophase both isoforms colocalized to the contractile ring. The different subcellular localizations of MHC-A and MHC-B, together with the data demonstrating that these myosins have markedly different enzymatic activities, strongly suggests that they have different functions.

Myosin heavy chain isoforms account for variation in pork quality

Meat Science ◽  
2002 ◽  
Vol 61 (2) ◽  
pp. 117-126 ◽  
Author(s):  
J.M. Eggert ◽  
F.F.S. Depreux ◽  
A.P. Schinckel ◽  
A.L. Grant ◽  
D.E. Gerrard
Keyword(s):  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Myosin Heavy Chain Isoforms ◽  
Pork Quality

The effect of glucocorticoids on the myosin heavy chain isoforms’ turnover in skeletal muscle

2003 ◽  
Vol 86 (2) ◽  
pp. 201-206 ◽  
Author(s):  
Teet Seene ◽  
Priit Kaasik ◽  
Ando Pehme ◽  
Karin Alev ◽  
Eva-Maria Riso
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Myosin Heavy Chain Isoforms

Myosin Heavy Chain Isoforms in Human Extraocular Muscles

2003 ◽  
Vol 44 (4) ◽  
pp. 1419 ◽  
Author(s):  
Daniel Kjellgren ◽  
Lars-Eric Thornell ◽  
Jesper Andersen ◽  
Fatima Pedrosa-Domello¨f
Keyword(s):  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Myosin Heavy Chain Isoforms ◽  
Extraocular Muscles

scholarly journals Expression of myosin heavy chain isoforms mRNA transcripts in the temporalis muscle of common chimpanzees ( Pan troglodytes )

2017 ◽  
Vol 214 ◽  
pp. 80-85 ◽  
Author(s):  
Neus Ciurana ◽  
Rosa Artells ◽  
Carmen Muñoz ◽  
Júlia Arias-Martorell ◽  
Gaëlle Bello-Hellegouarch ◽  
...  
Keyword(s):  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Pan Troglodytes ◽  
Myosin Heavy Chain Isoforms ◽  
Temporalis Muscle ◽  
Mrna Transcripts

scholarly journals Heterogeneous postnatal transitions in myosin heavy chain isoforms within the rabbit temporalis muscle

2006 ◽  
Vol 288A (10) ◽  
pp. 1095-1104 ◽  
Author(s):  
Joannes A.M. Korfage ◽  
Tim Van Wessel ◽  
Geerling E.J. Langenbach ◽  
Theo M.G.J. Van Eijden
Keyword(s):  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Myosin Heavy Chain Isoforms ◽  
Temporalis Muscle
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