IN SITU HYBRIDIZATION AND IMMUNOGOLD LOCALIZATION OF VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2 ON THE PERICYTES OF THE CHICK CHORIOALLANTOIC MEMBRANE

Cytokine ◽  
2002 ◽  
Vol 17 (5) ◽  
pp. 262-265 ◽  
Author(s):  
Domenico Ribatti ◽  
Amauri Cruz ◽  
Beatrice Nico ◽  
Judith Favier ◽  
Angelo Vacca ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
In Situ Hybridization ◽  
Growth Factor ◽  
Chorioallantoic Membrane ◽  
Growth Factor Receptor ◽  
Vascular Endothelial ◽  
Immunogold Localization ◽  
Chick Chorioallantoic Membrane ◽  
Endothelial Growth Factor

scholarly journals A Case Report of Ischemic Stroke in a Patient with Metastatic Gastric Cancer Secondary to Treatment with the Vascular Endothelial Growth Factor Receptor-2 Inhibitor Ramucirumab

2016 ◽  
Vol 9 (2) ◽  
pp. 317-320 ◽  
Author(s):  
Michael E. Christiansen ◽  
Timothy Ingall ◽  
Edward C. Lew ◽  
Ramesh K. Ramanathan ◽  
Harshita R. Paripati
Keyword(s):  
Gastric Cancer ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Case Reports ◽  
Growth Factor Receptor ◽  
Metastatic Gastric Cancer ◽  
Vascular Endothelial ◽  
First Case ◽  
Endothelial Growth Factor

Ramucirumab is an antiangiogenesis agent targeting the vascular endothelial growth factor receptor-2 (VEGFR-2), approved to treat advanced gastric and colon cancer. In clinical trials, it was shown to cause a small increase in arterial thromboembolism compared to placebo, including cerebral and myocardial ischemia, which was not statistically significant. Detailed case reports are lacking and we here present one of the first case reports of stroke secondary to ramucirumab-induced in situ thrombosis.

scholarly journals Distribution of vascular endothelial growth factor (VEGF) and its binding sites at the maternal-fetal interface during gestation in pigs

Reproduction ◽  
2001 ◽  
pp. 753-760 ◽  
Author(s):  
DS Charnock-Jones ◽  
DE Clark ◽  
D Licence ◽  
K Day ◽  
FB Wooding ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
In Situ Hybridization ◽  
Growth Factor ◽  
Epithelial Cells ◽  
Ligand Binding ◽  
Vascular Endothelial ◽  
Epithelial Layers ◽  
Endothelial Growth Factor ◽  
Maternal Fetal Interface

Pigs show epitheliochorial placentation, in which the maternal uterine epithelium and the fetal trophectoderm become closely apposed. There is no invasion of trophoblast into the maternal tissue, and nutrient and waste exchange take place across two epithelial layers beneath which a complex network of capillaries forms. Later in gestation, the epithelial cells become indented by blood vessels, which greatly reduces the distance for diffusion between the two circulatory systems. Vascular endothelial growth factor is a secreted homodimeric angiogenic growth factor that is involved in physiological and pathological angiogenesis. Its receptors are generally restricted to endothelial cells. Ligand binding, in situ hybridization and immunohistochemistry were carried out in pig placenta throughout gestation to investigate the possible role of vascular endothelial growth factor and its receptors in non-invasive placentation. In situ hybridization and immunohistochemistry revealed that mRNA and immunoreactivity for vascular endothelial growth factor were localized in both maternal and fetal epithelial cells at the maternal-fetal interface and over the maternal glands, although the signal was generally weaker in the maternal glands. Ligand binding was used to localize for vascular endothelial growth factor receptors; no binding was observed over the maternal glands, but very strong binding was localized to the endometrial blood vessels. At the interface between maternal and fetal tissue, a similar pattern was observed whereby the numerous small capillaries at the bases of the two apposed epithelia bound vascular endothelial growth factor specifically. It is concluded that vascular endothelial growth factor produced by the maternal and fetal epithelial layers promotes the growth of capillaries locally, which would facilitate the development of two vascular networks for the efficient transfer of nutrients and waste products.

scholarly journals Anti-Angiogenic Effect of Asperchalasine A Via Attenuation of VEGF Signaling

Biomolecules ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 358 ◽  
Author(s):  
Jun Yeon Park ◽  
Young Seok Ji ◽  
Hucheng Zhu ◽  
Yonghui Zhang ◽  
Do Hwi Park ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Umbilical Vein ◽  
Chorioallantoic Membrane ◽  
Growth Factor Receptor ◽  
Tube Formation ◽  
Dependent Manner ◽  
Vascular Endothelial ◽  
Concentration Dependent Manner ◽  
Endothelial Growth Factor

Cytochalasans are a group of structurally diverse fungal polyketide-amino acid hybrid metabolites that exhibit diverse biological functions. Asperchalasine A was identified and isolated from an extract of the marine-derived fungus, Aspergillus. Asperchalasine A is a cytochalasan dimer which consists of two cytochalasan molecules connected by an epicoccine. This study investigated the potential antiangiogenic effects of Aspergillus extract and asperchalasine A, which significantly inhibited cell adhesion and tube formation in human umbilical vein endothelial cells (HUVECs). Aspergillus extract and asperchalasine A decreased the vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor (VEGFR)-2 mRNA expression in a concentration-dependent manner. In addition, Aspergillus extract and asperchalasine A inhibited angiogenesis via downregulation of VEGF, p-p38, p-extracellular signal-regulated protein kinase (ERK), p-VEGFR-2, and p-Akt signaling pathways. Moreover, Aspergillus extract and asperchalasine A significantly inhibited the amount of blood vessel formation in fertilized chicken eggs using a chorioallantoic membrane assay. Our results provide experimental evidence of this novel biological activity of the potential antiangiogenic substances, Aspergillus extract, and asperchalasine A. This study also suggests that Aspergillus extract and its active component asperchalasine A are excellent candidates as adjuvant therapeutic substances for cancer prevention and treatment.

Moderate levels of ethanol induce expression of vascular endothelial growth factor and stimulate angiogenesis

2001 ◽  
Vol 281 (1) ◽  
pp. R365-R372 ◽  
Author(s):  
Jian-Wei Gu ◽  
Jesse Elam ◽  
Amanda Sartin ◽  
Wen Li ◽  
Raymond Roach ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Negative Impact ◽  
Chorioallantoic Membrane ◽  
Epidemiological Studies ◽  
Vascular Endothelial ◽  
Protective Effects ◽  
Vegf Mrna ◽  
Chick Chorioallantoic Membrane ◽  
Endothelial Growth Factor

Alcohol abuse has a negative impact on human health; however, epidemiological studies show that moderate consumption of ethanol (EtOH) reduces the risk of coronary heart disease, sudden cardiac death, and ischemic stroke. The mechanisms for these reductions in cardiovascular disease are not well established. Using cultured coronary artery vascular smooth muscle cells, we found that moderate levels of EtOH (10 and 20 mM) caused dose-related increases in both vascular endothelial growth factor (VEGF) mRNA (Northern blot) expression (1.9- and 2.6-fold) and VEGF protein (ELISA) expression (19 and 68%) compared with control ( P < 0.05). EtOH at 0.25 g · kg−1 · day−1 (7 days) increased VEGF mRNA expression by 1.48-fold over control, and increased vessel length density from 3.9 ± 0.7 (control) to 6.0 ± 0.3 mm/mm2 ( P < 0.05) in chick chorioallantoic membrane (CAM). We conclude that moderate levels of ethanol can induce VEGF expression and stimulate angiogenesis in chick CAM. Therefore, the results provide a theoretical basis for speculating that the cardiovascular-protective effects of moderate alcohol consumption may be partly mediated through VEGF-induced angiogenesis.

scholarly journals Vascular Permeability Factor/Vascular Endothelial Growth Factor and Vascular Stroma Formation in Neoplasia: Insights from In Situ Hybridization Studies

1998 ◽  
Vol 46 (5) ◽  
pp. 569-575 ◽  
Author(s):  
Lawrence F. Brown ◽  
Anthony J. Guidi ◽  
Kathi Tognazzi ◽  
Harold F. Dvorak
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
In Situ Hybridization ◽  
Growth Factor ◽  
Vascular Permeability ◽  
Vascular Endothelial ◽  
Vascular Permeability Factor ◽  
Permeability Factor ◽  
Endothelial Growth Factor ◽  
Vascular Stroma

The formation of vascular stroma plays an important role in the pathophysiology of malignancy. We describe the use of in situ hybridization in our laboratory as a tool to study the role of vascular permeability factor/vascular endothelial growth factor in the angiogenesis associated with malignancy.

Sign in / Sign up

Export Citation Format

Share Document