INTERLEUKIN-1β-INDUCED NITRIC OXIDE PRODUCTION AND INHIBITION OF INSULIN SECRETION IN RAT ISLETS OF LANGERHANS IS DEPENDENT UPON THE NITRIC OXIDE SYNTHASE COFACTOR TETRAHYDROBIOPTERIN

Cytokine ◽  
2002 ◽  
Vol 18 (2) ◽  
pp. 81-85 ◽  
Author(s):  
P. Stickings ◽  
J.M. Cunningham
Keyword(s):  
Nitric Oxide ◽  
Insulin Secretion ◽  
Nitric Oxide Synthase ◽  
Islets Of Langerhans ◽  
Interleukin 1Β ◽  
Nitric Oxide Production ◽  
Rat Islets ◽  
Oxide Production ◽  
Rat Islets Of Langerhans ◽  
Oxide Synthase

Nicotinamide Prevents Interleukin-1 Effects on Accumulated Insulin Release and Nitric Oxide Production in Rat Islets of Langerhans

Diabetes ◽  
1994 ◽  
Vol 43 (6) ◽  
pp. 770-777 ◽  
Author(s):  
H. U. Andersen ◽  
K. H. Jorgensen ◽  
J. Egeberg ◽  
T. Mandrup-Poulsen ◽  
J. Nerup
Keyword(s):  
Nitric Oxide ◽  
Insulin Release ◽  
Islets Of Langerhans ◽  
Interleukin 1 ◽  
Nitric Oxide Production ◽  
Rat Islets ◽  
Oxide Production ◽  
Rat Islets Of Langerhans

Cloning and Expression of Cytokine-Inducible Nitric Oxide Synthase cDNA From Rat Islets of Langerhans

Diabetes ◽  
1995 ◽  
Vol 44 (7) ◽  
pp. 753-758 ◽  
Author(s):  
A. E. Karlsen ◽  
H. U. Andersen ◽  
H. Vissing ◽  
P. Mose Larsen ◽  
S. J. Fey ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Islets Of Langerhans ◽  
Inducible Nitric Oxide Synthase ◽  
Cloning And Expression ◽  
Rat Islets ◽  
Rat Islets Of Langerhans ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Interleukin-1β-induced nitric oxide production from isolated rat islets is modulated by D-glucose and 3-isobutyl-1-methyl xanthine

1996 ◽  
Vol 134 (2) ◽  
pp. 251-259 ◽  
Author(s):  
Henrik U Andersen ◽  
Dídac Mauricio ◽  
Allan E Karlsen ◽  
Thomas Mandrup-Poulsen ◽  
Jens H Nielsen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inducible Nitric Oxide Synthase ◽  
Interleukin 1Β ◽  
Nitric Oxide Production ◽  
Rat Islets ◽  
Oxide Production ◽  
Nitrite Production ◽  
Oxide Synthase ◽  
Methyl Xanthine ◽  
Inducible Nitric Oxide

Andersen HU, Mauricio D, Karlsen AE, Mandrup-Poulsen T, Nielsen JH, Nerup J. Interleukin-nitric oxide production from isolated rat islets is modulated by d-glucose and 3-isobutyl-1-methyl xanthine. Eur J Endocrinol 1996:134:251–9. ISSN 0804–4643 Interleukin-1β has been proposed to cause selective β-cell destruction via the induction of nitric oxide synthesis. The cytotoxic effect of interleukin-1β is modulated by the concentration of d-glucose in the medium. The aim of this study was to investigate if d-glucose-mediated modulation of interleukin-1β effects on insulin release from isolated rat islets was related to modulation of nitric oxide production. Further, we wished to investigate the effects of agents increasing the intracellular concentration of cAMP on interleukin-1β-induced nitrite production. We demonstrated that d-glucose potentiated interleukin-1β-induced nitrite production in rat islets without affecting the mRNA level of the inducible nitric oxide synthase. This effect was dissociated from interleukin-1β action on insulin release, since a relative protection against interleukin-1β effects on acute insulin release was found at high (28 mmol/l) concentrations of d-glucose, and blocking nitrite production by the L-arginine analog aminoguanidine, which selectively inhibits the cytokine-inducible nitric oxide synthase, did not result in protection against the inhibitory action of interleukin-1β Neither l-glucose nor the secretagogues l-leucine, tolbutamide and β-isobutyl-1-methyl xanthine shared the potentiating effect of d-glucose, The phosphodiesterase inhibitor β-isobutyl-1-methyl xanthine reduced interleukin-1β-induced nitrite production at 3.3 mmol/l d-glucose, an effect that could be reproduced by the cAMP analog dibutyryl cAMP. Addition of 3-isobutyl-1-methyl xanthine resulted in a threefold reduction in the mRNA level of interleukin-1β-induced inducible nitric oxide synthase. We conclude that interleukin-1β-induced islet nitric oxide synthesis is augmented by d-glucose but not by non-substrate secretagogues, and that secretagogues that elevate cAMP inhibit islet nitric oxide production. Jørn Nerup, Steno Diabetes Center, Niels Steensens vej 2, DK-2820 Gentofte, Denmark

Cloning and expression of cytokine-inducible nitric oxide synthase cDNA from rat islets of Langerhans

Diabetes ◽  
1995 ◽  
Vol 44 (7) ◽  
pp. 753-758 ◽  
Author(s):  
A. E. Karlsen ◽  
H. U. Andersen ◽  
H. Vissing ◽  
P. M. Larsen ◽  
S. J. Fey ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Islets Of Langerhans ◽  
Inducible Nitric Oxide Synthase ◽  
Cloning And Expression ◽  
Rat Islets ◽  
Rat Islets Of Langerhans ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide
Sign in / Sign up

Export Citation Format

Share Document