Cytosolic pH Variations in Perfused Rat Liver at 4°C: Role of Intracellular Buffering Power

Cryobiology ◽  
1998 ◽  
Vol 36 (4) ◽  
pp. 269-278 ◽  
Author(s):  
Thierry Durand ◽  
Giovanni Vidal ◽  
Paul Canioni ◽  
Jean-Louis Gallis
Keyword(s):  
Rat Liver ◽  
Perfused Rat Liver ◽  
Cytosolic Ph ◽  
Buffering Power ◽  
Ph Variations

Cytosolic pH regulation in perfused rat liver: role of intracellular bicarbonate production

1998 ◽  
Vol 1425 (1) ◽  
pp. 224-234 ◽  
Author(s):  
Giovanni Vidal ◽  
Thierry Durand ◽  
Paul Canioni ◽  
Jean-Louis Gallis
Keyword(s):  
Rat Liver ◽  
Ph Regulation ◽  
Perfused Rat Liver ◽  
Cytosolic Ph

scholarly journals Ganglioside incorporation and release by the isolated perfused rat liver

1991 ◽  
Vol 274 (2) ◽  
pp. 581-585 ◽  
Author(s):  
S C Kivatinitz ◽  
A Miglio ◽  
R Ghidoni
Keyword(s):  
Rat Liver ◽  
The Other ◽  
Regulatory Role ◽  
Isolated Perfused Rat Liver ◽  
Order Kinetic ◽  
Perfused Rat Liver ◽  
Ganglioside Gm1 ◽  
First Order ◽  
Pseudo First Order

The fate of exogenous ganglioside GM1 labelled in the sphingosine moiety, [Sph-3H]GM1, administered as a pulse, in the isolated perfused rat liver was investigated. When a non-recirculating protocol was employed, the amount of radioactivity in the liver and perfusates was found to be dependent on the presence of BSA in the perfusion liquid and on the time elapsed after the administration of the ganglioside. When BSA was added to the perfusion liquid, less radioactivity was found in the liver and more in the perfusate at each time tested, for up to 1 h. The recovery of radioactivity in the perfusates followed a complex course which can be described by three pseudo-first-order kinetic constants. The constants, in order of decreasing velocity, are interpreted as: (a) the dilution of the labelled GM1 by the constant influx of perfusion liquid; (b) the washing off of GM1 loosely bound to the surface of liver cells; (c) the release of gangliosides from the liver. Process (b) was found to be faster in the presence of BSA, probably owing to the ability of BSA to bind gangliosides. The [Sph-3H]GM1 in the liver underwent metabolism, leading to the appearance of products of anabolic (GD1a, GD1b) and catabolic (GM2, GM3) origin; GD1a appeared before GM2 and GM3 but, at times longer than 10 min, GM2 and GM3 showed more radioactivity than GD1a. At a given time the distribution of the radioactivity in the perfusates was quite different from that of the liver. In fact, after 60 min GD1a was the only metabolite present in any amount, the other being GM3, the quantity of which was small. This indicates that the liver is able to release newly synthesized gangliosides quite specifically. When a recirculating protocol was used, there were more catabolites and less GD1a than with the non-recirculating protocol. A possible regulatory role of ganglioside re-internalization on their own metabolism in the liver is postulated.

Role of pyruvate transporter in the regulation of pyruvate dehydrogenase multienzyme complex in perfused rat liver

Biochemistry ◽  
1982 ◽  
Vol 21 (2) ◽  
pp. 346-353 ◽  
Author(s):  
Franz Maximilian Zwiebel ◽  
Ursula Schwabe ◽  
Merle S. Olson ◽  
Roland Scholz
Keyword(s):  
Rat Liver ◽  
Pyruvate Dehydrogenase ◽  
Multienzyme Complex ◽  
Perfused Rat Liver

Role of neutrophils in ischemia/reperfusion liver injury. A study in the isolated perfused rat liver

1991 ◽  
Vol 13 ◽  
pp. S109
Author(s):  
O. Chazouillères ◽  
C. Legendre ◽  
M. Vaubourdolle ◽  
M.T. Bonnefis ◽  
C. Rey ◽  
...  
Keyword(s):  
Liver Injury ◽  
Rat Liver ◽  
Ischemia Reperfusion ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver

scholarly journals The action of α-adrenergic agonists on plasma-membrane calcium fluxes in perfused rat liver

1984 ◽  
Vol 220 (1) ◽  
pp. 43-50 ◽  
Author(s):  
P H Reinhart ◽  
W M Taylor ◽  
F L Bygrave
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Potential Role ◽  
Dependent Manner ◽  
Perfused Rat Liver ◽  
Adrenergic Agonists ◽  
Mediated Effects ◽  
Alpha Agonist ◽  
Alpha Adrenergic Agonist

The effect of alpha-adrenergic agonists on Ca2+ fluxes was examined in the perfused rat liver by using a combination of Ca2+-electrode and 45Ca2+-uptake techniques. We showed that net Ca2+ fluxes can be described by the activities of separate Ca2+-uptake and Ca2+-efflux components, and that alpha-adrenergic agonists modulate the activity of both components in a time-dependent manner. Under resting conditions, Ca2+-uptake and -efflux activities are balanced, resulting in Ca2+ cycling across the plasma membrane. The alpha-adrenergic agonists vasopressin and angiotensin, but not glucagon, stimulate the rate of both Ca2+ efflux and Ca2+ uptake. During the first 2-3 min of alpha-agonist administration the effect on the efflux component is the greater, the net effect being efflux of Ca2+ from the cell. After 3-4 min of phenylephrine treatment, net Ca2+ movements are essentially complete, however, the rate of Ca2+ cycling is significantly increased. After removal of the alpha-agonist a large stimulation of the rate of Ca2+ uptake leads to the net accumulation of Ca2+ by the cell. The potential role of these Ca2+ flux changes in the expression of alpha-adrenergic-agonist-mediated effects is discussed.

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