The Phosphodiesterase Inhibitors Pentoxifylline and Rolipram Suppress Macrophage Activation and Nitric Oxide Production in Vitro and in Vivo

2001 ◽  
Vol 98 (2) ◽  
pp. 272-279 ◽  
Author(s):  
Evette Beshay ◽  
France Croze ◽  
Gérald J. Prud'homme
Keyword(s):  
Nitric Oxide ◽  
Macrophage Activation ◽  
Phosphodiesterase Inhibitors ◽  
Nitric Oxide Production ◽  
Oxide Production

Effects of Periodic Body Acceleration on the In Vivo Vasoactive Response to N-w-nitro–L-arginine and the In Vitro Nitric Oxide Production

2003 ◽  
Vol 31 (11) ◽  
pp. 1337-1346 ◽  
Author(s):  
Jose A. Adams ◽  
James E. Moore, Jr. ◽  
Michael R. Moreno ◽  
Jaqueline Coelho ◽  
Jorge Bassuk ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Production ◽  
Body Acceleration ◽  
Oxide Production

scholarly journals The 12-HHT/BLT2/NO Axis Is Associated to the Wound Healing and Skin Condition in Different Glycaemic States

2019 ◽  
Vol 7 (4) ◽  
pp. 65
Author(s):  
Leguina-Ruzzi ◽  
Ortiz Diban ◽  
Velarde
Keyword(s):  
Nitric Oxide ◽  
Wound Healing ◽  
Tight Junction ◽  
Skin Condition ◽  
Inos Mrna ◽  
Nitric Oxide Production ◽  
Mrna Levels ◽  
Oxide Production

Type 2 diabetes affects over 340 million people worldwide. This condition can go unnoticed and undiagnosed for years, leading to a late stage where high glycaemia produces complications such as delayed wound healing. Studies have shown that 12-HHT through BLT2, accelerates keratinocyte migration and wound healing. Additionally, evidence has shown the role of nitric oxide as a pro-regenerative mediator, which is decreased in diabetes. Our main goal was to study the association between the 12-HHT/BLT2 axis and the nitric oxide production in wound healing under different glycaemia conditions. For that purpose, we used in vivo and in vitro models. Our results show that the skin from diabetic mice showed reduced BLT2 and iNOS mRNA, TEER, 12-HHT, nitrites, and tight junction levels, accompanied by higher MMP9 mRNA levels. Furthermore, a positive correlation between BLT2 mRNA and nitrites was observed. In vitro, HaCaT-BLT2 cells showed higher nitric oxide and tight junction levels, and reduced MMP9 mRNA levels, compared to mock-keratinocytes under low and high glucose condition. The wound healing capacity was associated with higher nitric oxide production and was affected by the NOS inhibition. We suggest that the BLT2 expression improves the keratinocyte response to hyperglycaemia, associated with the production of nitric oxide.

scholarly journals Nitric Oxide Production and Fc Receptor-Mediated Phagocytosis as Functional Readouts of Macrophage Activity upon Stimulation with Inactivated Poultry Vaccines In Vitro

Vaccines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 332 ◽  
Author(s):  
Robin van den Biggelaar ◽  
Willem van Eden ◽  
Victor Rutten ◽  
Christine Jansen
Keyword(s):  
Nitric Oxide ◽  
Allantoic Fluid ◽  
Fc Receptor ◽  
Vaccine Antigen ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Future Assessment ◽  
Batch Release

Vaccine batches must pass routine quality control to confirm that their ability to induce protection against disease is consistent with batches of proven efficacy from development studies. For poultry vaccines, these tests are often performed in laboratory chickens by vaccination-challenge trials or serological assays. The aim of this study was to investigate innate immune responses against inactivated poultry vaccines and identify candidate immune parameters for in vitro quality tests as alternatives for animal-based quality tests. For this purpose, we set up assays to measure nitric oxide production and phagocytosis by the macrophage-like cell line HD11, upon stimulation with inactivated poultry vaccines for infectious bronchitis virus (IBV), Newcastle disease virus (NDV), and egg drop syndrome virus (EDSV). In both assays, macrophages became activated after stimulation with various toll-like receptor agonists. Inactivated poultry vaccines stimulated HD11 cells to produce nitric oxide due to the presence of mineral oil adjuvant. Moreover, inactivated poultry vaccines were found to enhance Fc receptor-mediated phagocytosis due to the presence of allantoic fluid in the vaccine antigen preparations. We showed that inactivated poultry vaccines stimulated nitric oxide production and Fc receptor-mediated phagocytosis by chicken macrophages. Similar to antigen quantification methods, the cell-based assays described here can be used for future assessment of vaccine batch-to-batch consistency. The ability of the assays to determine the immunopotentiating properties of inactivated poultry vaccines provides an additional step in the replacement of current in vivo batch-release quality tests.

Extract of the seed coat of Tamarindus indica inhibits nitric oxide production by murine macrophages in vitro and in vivo

2004 ◽  
Vol 42 (4) ◽  
pp. 649-658 ◽  
Author(s):  
T. Komutarin ◽  
S. Azadi ◽  
L. Butterworth ◽  
D. Keil ◽  
B. Chitsomboon ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Seed Coat ◽  
Nitric Oxide Production ◽  
Tamarindus Indica ◽  
Murine Macrophages ◽  
Oxide Production

In vivo production of nitric oxide correlates with NMDA-induced cerebral hyperemia in newborn sheep

1995 ◽  
Vol 269 (1) ◽  
pp. H215-H221 ◽  
Author(s):  
F. J. Northington ◽  
J. R. Tobin ◽  
R. C. Koehler ◽  
R. J. Traystman
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Production ◽  
Nitric Oxide Synthase Inhibitor ◽  
Oxide Production ◽  
Adenosine Receptor Agonist ◽  
Synthase Inhibitor ◽  
Oxide Synthase ◽  
Hydrogen Clearance

Stimulation of N-methyl-D-aspartate (NMDA) receptors in brain increases nitric oxide production in vitro. We tested the hypothesis that nitric oxide participates in the increase in local cerebral blood flow (CBF) caused by infusion of NMDA in anesthetized newborn sheep. We used the combined hydrogen clearance and microdialysis technique for simultaneous measurement of local CBF, infusion of drugs, and measurement of interstitial levels of L-[14C]citrulline in the parietal cortex. Release of L-[14C]citrulline into the dialysate during continuous infusion of L-[14C]arginine was used as a marker of nitric oxide production in vivo. Citrulline recovery and CBF were measured hourly during a 4-h infusion of cerebrospinal fluid containing either 1) no additional drugs, 2) 1 mM NMDA, 3) 1 mM NG-nitro-L-arginine methyl ester (L-NAME, a nitric oxide synthase inhibitor), 4) 1 mM NMDA + 1 mM L-NAME, 5) 0.1 mM 2-chloroadenosine (adenosine receptor agonist), or 6) 0.1 mM 2-chloroadenosine + 1 mM L-NAME. At 240 min of perfusion, CBF (ml.min-1.100 g-1; means +/- SE) was as follows: control 52 +/- 3, NMDA 116 +/- 11, L-NAME 32 +/- 5, NMDA+L-NAME 40 +/- 4,2-chloroadenosine 201 +/- 63, and 2-chloroadenosine+L-NAME 129 +/- 18. Citrulline recovery (fmol/min) at 240 min of perfusion was as follows: control 38 +/- 12, NMDA 149 +/- 21, L-NAME 9 +/- 1, NMDA+L-NAME 39 +/- 5, 2-chloroadenosine 13 +/- 5, and 2-chloroadenosine+L-NAME 17 +/- 1.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivo

2015 ◽  
Vol 65 (4) ◽  
pp. 413-426 ◽  
Author(s):  
Mircea Dumitru Croitoru ◽  
Hermina Iulia Petkes ◽  
Ibolya Fülöp ◽  
Remus Cotârlan ◽  
Oana Elena Şerban ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Spin Trap ◽  
Nitrate Concentration ◽  
Plasma Concentrations ◽  
Hepatic Metabolism ◽  
Nitric Oxide Production ◽  
Spin Traps ◽  
Oxide Production

Abstract Free radicals are involved in the development of reperfusion injuries. Using a spin trap, the intensity of such lesions can be reduced. Nitrones (effective in vivo spin traps) were tried in this work as in vivo nitric oxide donors. Nitrite and nitrate concentration values (rabbit blood) were used as biomarkers of nitric oxide production. Most nitrones did not increase plasma concentrations of nitrite and nitrate; on the contrary, reduced plasma concentrations of these indicators were noted. However, glyoxal isopropyldinitrone, in a dose of 50 mg kg-1, was highly effective in increasing nitric oxide production. At the same time, nitrones do not react with hepatic homogenates, proving that the release of nitric oxide takes place in the tissues and is not related to hepatic metabolism. Before using nitrones in vivo, they were tested in vitro for the ability to release nitric oxide following a reaction with the hydroxyl radical.

DT-13 ameliorates TNF-α-induced nitric oxide production in the endothelium in vivo and in vitro

2018 ◽  
Vol 495 (1) ◽  
pp. 1175-1181 ◽  
Author(s):  
Ruiping Fan ◽  
Yuwei Han ◽  
Han Han ◽  
Zhengdong Chen ◽  
Boyang Yu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Tnf Α

Inhibition of macrophage nitric oxide production by tetrahydrocannabinol in vivo and in vitro

1996 ◽  
Vol 18 (12) ◽  
pp. 749-752 ◽  
Author(s):  
Ronald G. Coffey ◽  
Elizabeth Snella ◽  
Karen Johnson ◽  
Susan Pross
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Production ◽  
Oxide Production
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