Antigen-Dependent Cytokine mRNA Expression by Individual Rhesus Macaque T Helper Cells by Flow Cytometry

2000 ◽  
Vol 201 (2) ◽  
pp. 94-108 ◽  
Author(s):  
Juan Arredondo ◽  
F. Xusheng Lü ◽  
Francois Villinger ◽  
Michael B. McChesney ◽  
Jerry R. McGhee ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Mrna Expression ◽  
Rhesus Macaque ◽  
T Helper Cells ◽  
T Helper ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression ◽  
Helper Cells

Molecular, biochemical characterization and assessment of immunogenic potential of cofactor-independent phosphoglycerate mutase againstLeishmania donovani: a step towards exploring novel vaccine candidate

Parasitology ◽  
2017 ◽  
Vol 145 (4) ◽  
pp. 508-526 ◽  
Author(s):  
RATI TANDON ◽  
SHARAT CHANDRA ◽  
RAJENDRA KUMAR BAHARIA ◽  
PRAGYA MISRA ◽  
SANCHITA DAS ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Mrna Expression ◽  
T Helper Cells ◽  
Clinical Remission ◽  
Vaccine Candidate ◽  
No Production ◽  
T Helper ◽  
Prophylactic Efficacy ◽  
Helper Cells ◽  
Ifn Γ

SUMMARYDespite immense efforts, vaccine against visceral leishmaniasis has yet not been developed. Earlier our proteomic study revealed a novel protein, cofactor-independent phoshoglycerate mutase (LdiPGAM), an important enzyme in glucose metabolism, in T helper cells type 1 (Th1) stimulatory region of solubleLeishmania donovaniantigen. In this study, LdiPGAM was biochemically and molecularly characterized and evaluated for its immunogenicity and prophylactic efficacy againstL. donovani. Immunogenicity of recombinant LdiPGAM (rLdiPGAM) was initially assessed in naïve hamsters immunized with it by analysing mRNA expression of inducible nitric oxide (NO) synthase (iNOS) and other Th1/T helper cells type 2 cytokines, which revealed an upregulation of Th1 cytokines along with iNOS. Immunogenicity of rLdiPGAM was further evaluated in lymphocytes of treatedLeishmania-infected hamsters and peripheral blood mononuclear cells ofLeishmaniapatients in clinical remission by various parameters, viz. lymphoproliferation assay and NO production (hamsters and patients) and levels of various cytokines (patients). rLdiPGAM induced remarkable Lymphoproliferative response and NO production in treatedLeishmania-infected hamsters as well as in patients and increase in interferon gamma (IFN-γ), interleukin-12 (IL-12p40) responses inLeishmaniapatients in clinical remission. Vaccination with rLdiPGAM exerted considerable prophylactic efficacy (73%) supported by increase in mRNA expression of iNOS, IFN-γ and IL-12p40 with decrease in transforming growth factor beta and interleukin-10. Above results indicate the importance of rLdiPGAM protein as a potential vaccine candidate against visceral leishmaniasis.

scholarly journals Latex allergy in a dental nurse: Late nasal response is associated with eosinophil recruitment and T helper 2 cell type cytokine mRNA expression

1998 ◽  
Vol 47 (2) ◽  
pp. 103-107 ◽  
Author(s):  
Keisuke Masuyama ◽  
Mikila R. Jacobson ◽  
Paul Cullinan ◽  
Julie Cannon ◽  
Anthony J. Newman Taylor ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Latex Allergy ◽  
Cell Type ◽  
T Helper ◽  
Cytokine Mrna ◽  
Eosinophil Recruitment ◽  
T Helper 2 ◽  
Cytokine Mrna Expression

scholarly journals Is myometrial inflammation a cause or a consequence of term human labour?

2017 ◽  
Vol 235 (1) ◽  
pp. 69-83 ◽  
Author(s):  
Natasha Singh ◽  
Bronwen Herbert ◽  
Gavin R Sooranna ◽  
Nicolas M Orsi ◽  
Lydia Edey ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Mrna Expression ◽  
Inflammatory Cell ◽  
Preterm Labour ◽  
Cytokine Mrna ◽  
Protein Levels ◽  
Cytokine Mrna Expression ◽  
Spontaneous Labour ◽  
Human Labour ◽  
Term Labour

Myometrial inflammation is thought to have a pivotal role in the onset of term and some forms of preterm labour. This is based on the comparison of samples taken from women undergoing term elective CS prior to the onset of labour with those taken from women in established labour. Consequently, it is not clear whether myometrial inflammation is a cause or a consequence of labour. Our objective is to test the hypothesis that myometrial inflammation is a consequence of the onset of labour. To test this hypothesis, we have obtained myometrial samples from women at various stages of pregnancy and spontaneous labour and studied the activation of the AP-1 (c-Jun) and NFκB (p65) systems, cytokine mRNA expression and protein levels and inflammatory cell infiltration and activation. We found that the activation of p65 declined from preterm to term not in labour samples and thereafter increased in early and established labour. Cytokine mRNA expression and protein levels increased in established labour only. Using flow cytometry of myometrial tissue, we found that the number of neutrophils did increase with the onset of labour, but on tissue section, these were seen to be intravascular and not infiltrating into the myometrium. These data suggest that myometrial inflammation is a consequence rather than a cause of term labour.

Type I Interferons promote maintenance and function of follicular T helper cells in vitro

2019 ◽  
Author(s):  
S Ehrlich ◽  
K Wild ◽  
M Smits ◽  
K Zoldan ◽  
M Hofmann ◽  
...  
Keyword(s):  
T Helper Cells ◽  
Type I Interferons ◽  
Type I ◽  
T Helper ◽  
Helper Cells ◽  
Follicular T Helper Cells ◽  
And Function
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