Characterization of a New Human Macrophage Cell Line 2MAC

1997 ◽  
Vol 177 (2) ◽  
pp. 182-193 ◽  
Author(s):  
Deno P. Dialynas ◽  
Peter C. Tan ◽  
Gregory D. Huhn ◽  
John Yu
Keyword(s):  
Cell Line ◽  
Human Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell

scholarly journals Characterization of the C1q receptor on a human macrophage cell line, U937

1984 ◽  
Vol 218 (2) ◽  
pp. 547-555 ◽  
Author(s):  
J Arvieux ◽  
A Reboul ◽  
J C Bensa ◽  
M G Colomb
Keyword(s):  
Cell Line ◽  
Lipid A ◽  
Membrane Receptors ◽  
Human Macrophage ◽  
Macrophage Cell Line ◽  
Binding Studies ◽  
Macrophage Cell ◽  
Reversible Binding ◽  
Inhibition Studies

The binding of C1q to the human macrophage cell line U937 has been studied. Fluorescence microscopy with fluorescein-conjugated F(ab')2 anti-C1q antibody showed that 100% of the cell population is able to bind exogenous C1q. Monomeric C1q binding to U937 cells is very weak at normal ionic strength (I0.15) and was therefore investigated at I0.07, conditions which stabilize the binding. However, aggregation of C1q on dextran sulphate or a lipid A-rich lipopolysaccharide allowed a firm, binding at I0.15. Quantitative binding studies with monomeric 125I-C1q showed a concentration-dependent, saturable, specific and reversible binding involving specific membrane receptors. Scatchard plots of C1q binding indicated [1.6 +/- 0.7 (1 S.D.)] X 10(6) sites per cell with an equilibrium constant of (2.9 +/- 1.8) X 10(7) M-1 at I0.07. The location of the molecule region mediating C1q binding was established with collagen-like fragments prepared by partial pepsin digestion, confirming earlier results obtained by inhibition studies.

Modulation of Fcγ receptors on the human macrophage cell line U-937

1989 ◽  
Vol 120 (1) ◽  
pp. 174-187 ◽  
Author(s):  
Milton D. Rossman ◽  
Edgar Chen ◽  
Paul Chien ◽  
Alan D. Schreiber
Keyword(s):  
Cell Line ◽  
Human Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Fcγ Receptors

scholarly journals Characterisation of a New Human Alveolar Macrophage-Like Cell Line (Daisy)

Lung ◽  
2019 ◽  
Vol 197 (6) ◽  
pp. 687-698
Author(s):  
Laura R. Sadofsky ◽  
Yvette A. Hayman ◽  
Jesse Vance ◽  
Jorge L. Cervantes ◽  
Simon D. Fraser ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Line ◽  
Ex Vivo ◽  
Human Macrophage ◽  
Macrophage Cell Line ◽  
Binding Assays ◽  
Macrophage Cell ◽  
Mitogenic Stimulation ◽  
Human Alveolar Macrophage

Abstract Purpose There is currently no true macrophage cell line and in vitro experiments requiring these cells currently require mitogenic stimulation of a macrophage precursor cell line (THP-1) or ex vivo maturation of circulating primary monocytes. In this study, we characterise a human macrophage cell line, derived from THP-1 cells, and compare its phenotype to the THP-1 cells. Methods THP-1 cells with and without mitogenic stimulation were compared to the newly derived macrophage-like cell line (Daisy) using microscopy, flow cytometry, phagocytosis assays, antigen binding assays and gene microarrays. Results We show that the cell line grows predominantly in an adherent monolayer. A panel of antibodies were chosen to investigate the cell surface phenotype of these cells using flow cytometry. Daisy cells expressed more CD11c, CD80, CD163, CD169 and CD206, but less CD14 and CD11b compared with mitogen-stimulated THP-1 cells. Unlike stimulated THP-1 cells which were barely able to bind immune complexes, Daisy cells showed large amounts of immune complex binding. Finally, although not statistically significant, the phagocytic ability of Daisy cells was greater than mitogen-stimulated THP-1 cells, suggesting that the cell line is more similar to mature macrophages. Conclusions The observed phenotype suggests that Daisy cells are a good model of human macrophages with a phenotype similar to human alveolar macrophages.

Establishment and characterization of macrophage cell line from thymus ofCatla catla(Hamilton, 1822)

2012 ◽  
Vol 45 (2) ◽  
pp. 299-311 ◽  
Author(s):  
Dharmendra Kumar Chaudhary ◽  
Neeraj Sood ◽  
Gaurav Rathore ◽  
Pravata Kumar Pradhan ◽  
Peyush Punia ◽  
...  
Keyword(s):  
Cell Line ◽  
Macrophage Cell Line ◽  
Macrophage Cell

Reduction of Sb(V) in a Human Macrophage Cell Line Measured by HPLC-ICP-MS

2011 ◽  
Vol 144 (1-3) ◽  
pp. 234-243 ◽  
Author(s):  
Claus Hansen ◽  
Erik Wind Hansen ◽  
Helle Rüsz Hansen ◽  
Bente Gammelgaard ◽  
Stefan Stürup
Keyword(s):  
Cell Line ◽  
Human Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Icp Ms
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