Expression and Biological Activities of Bovine Interleukin 4: Effects of Recombinant Bovine Interleukin 4 on T Cell Proliferation and B Cell Differentiation and Proliferation in Vitro

1995 ◽  
Vol 163 (2) ◽  
pp. 268-279 ◽  
Author(s):  
D.Mark Estes ◽  
Ayumi Hirano ◽  
Volker T. Heussler ◽  
Dirk A.E. Dobbelaere ◽  
Wendy C. Browns
Keyword(s):  
Cell Proliferation ◽  
Cell Differentiation ◽  
B Cell ◽  
Biological Activities ◽  
Interleukin 4 ◽  
T Cell Proliferation ◽  
B Cell Differentiation ◽  
Differentiation And Proliferation ◽  
Proliferation In Vitro

scholarly journals The impact of von Willebrand factor on factor VIII memory immune responses

2017 ◽  
Vol 1 (19) ◽  
pp. 1565-1574 ◽  
Author(s):  
Juan Chen ◽  
Jocelyn A. Schroeder ◽  
Xiaofeng Luo ◽  
Qizhen Shi
Keyword(s):  
Cell Proliferation ◽  
Cell Differentiation ◽  
Immune Responses ◽  
B Cell ◽  
T Cell Proliferation ◽  
B Cell Differentiation ◽  
Key Points ◽  
Von Willebrand ◽  
The Impact ◽  
Willebrand Factor

Key Points VWF attenuates primed T-cell proliferation and memory B-cell differentiation. VWF mitigates FVIII memory responses in FVIIInull mice.

Antigen-loaded exosomes alone induce Th1-type memory through a B cell–dependent mechanism

Blood ◽  
2009 ◽  
Vol 113 (12) ◽  
pp. 2673-2683 ◽  
Author(s):  
Khaleda Rahman Qazi ◽  
Ulf Gehrmann ◽  
Emilie Domange Jordö ◽  
Mikael C. I. Karlsson ◽  
Susanne Gabrielsson
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
B Cell ◽  
T Cell Responses ◽  
T Cell Response ◽  
T Cell Proliferation ◽  
Cell Responses ◽  
Proliferation In Vitro

Abstract Exosomes are nanovesicles harboring proteins important for antigen presentation. We compared the potency of differently loaded exosomes, directly loaded with OVA323-339 peptide (Pep-Exo) or exosomes from OVA-pulsed DCs (OVA-Exo), for their ability to induce specific T-cell proliferation in vitro and in vivo. Both Pep-Exo and OVA-Exo elicited specific transgenic T-cell proliferation in vitro, with the Pep-Exo being more efficient. In contrast, only OVA-Exo induced specific T-cell responses in vivo highlighting the importance of indirect loading strategies in clinical applications. Coadministration of whole OVA overcame the unresponsiveness with Pep-Exo but still elicited a lower response compared with OVA-Exo. In parallel, we found that OVA-Exo not only augmented the specific T-cell response but also gave a Th1-type shift and an antibody response even in the absence of whole OVA. We detected IgG2a and interferon-γ production from splenocytes showing the capability of exosomes to provide antigen for B-cell activation. Furthermore, we found that B cells are needed for exosomal T-cell stimulation because Bruton tyrosine kinase–deficient mice showed abrogated B- and T-cell responses after OVA-Exo immunization. These findings reveal that exosomes are potent immune regulators and are relevant for the design of vaccine adjuvants and therapeutic intervention strategies to modulate immune responses.

scholarly journals Optimized Protocols for in Vitro T Cell-Dependent and T Cell-Independent Activation for B Cell Differentiation Studies Using Limited Cells

2021 ◽  
Author(s):  
Casper Marsman ◽  
Dorit Verhoeven
Keyword(s):  
Cell Differentiation ◽  
T Cell ◽  
B Cells ◽  
B Cell ◽  
Plasma Cells ◽  
Cell Formation ◽  
B Cell Differentiation ◽  
Differentiation Potential ◽  
Human B Cell

Background/methods: For mechanistic studies, in vitro human B cell differentiation and generation of plasma cells are invaluable techniques. However, the heterogeneity of both T cell-dependent (TD) and T cell-independent (TI) stimuli and the disparity of culture conditions used in existing protocols makes interpretation of results challenging. The aim of the present study was to achieve the most optimal B cell differentiation conditions using isolated CD19+ B cells and PBMC cultures. We addressed multiple seeding densities, different durations of culturing and various combinations of TD stimuli and TI stimuli including B cell receptor (BCR) triggering. B cell expansion, proliferation and differentiation was analyzed after 6 and 9 days by measuring B cell proliferation and expansion, plasmablast and plasma cell formation and immunoglobulin (Ig) secretion. In addition, these conditions were extrapolated using cryopreserved cells and differentiation potential was compared. Results: This study demonstrates improved differentiation efficiency after 9 days of culturing for both B cell and PBMC cultures using CD40L and IL-21 as TD stimuli and 6 days for CpG and IL-2 as TI stimuli. We arrived at optimized protocols requiring 2500 and 25.000 B cells per culture well for TD and TI assays, respectively. The results of the PBMC cultures were highly comparable to the B cell cultures, which allows dismissal of additional B cell isolation steps prior to culturing. In these optimized TD conditions, the addition of anti-BCR showed little effect on phenotypic B cell differentiation, however it interferes with Ig secretion measurements. Addition of IL-4 to the TD stimuli showed significantly lower Ig secretion. The addition of BAFF to optimized TI conditions showed enhanced B cell differentiation and Ig secretion in B cell but not in PBMC cultures. With this approach, efficient B cell differentiation and Ig secretion was accomplished when starting from fresh or cryopreserved samples. Conclusion: Our methodology demonstrates optimized TD and TI stimulation protocols for more indepth analysis of B cell differentiation in primary human B cell and PBMC cultures while requiring low amounts of B cells, making them ideally suited for future clinical and research studies on B cell differentiation of patient samples from different cohorts of B cell-mediated diseases.

A CD28 CDR3 peptide analog inhibits CD4+ T-cell proliferation in vitro

2006 ◽  
pp. 689-690
Author(s):  
Mythily Srinivasan ◽  
Richard M. Wardrop ◽  
Caroline C. Whitacre ◽  
Pravin T.P. Kaumaya
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
Cd4 T Cell ◽  
T Cell Proliferation ◽  
Peptide Analog ◽  
Proliferation In Vitro

scholarly journals Modulation of spontaneous B-cell differentiation in macroglobulinemia by retinoic acid

Blood ◽  
1994 ◽  
Vol 83 (8) ◽  
pp. 2206-2210 ◽  
Author(s):  
Y Levy ◽  
S Labaume ◽  
MC Gendron ◽  
JC Brouet
Keyword(s):  
Retinoic Acid ◽  
Cell Differentiation ◽  
B Cells ◽  
B Cell ◽  
Plasma Cells ◽  
B Cell Differentiation ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Culture Supernatants

Abstract We previously showed that clonal blood B cells from patients with macroglobulinemia spontaneously differentiate in vitro to plasma cells. This process is dependent on an interleukin (IL)-6 autocrine pathway. We investigate here whether all-trans-retinoic acid (RA) interferes with B-cell differentiation either in patients with IgM gammapathy of undetermined significance (MGUS) or Waldenstrom's macroglobulinemia (WM). RA at a concentration of 10(-5) to 10(-8) mol/L inhibited by 50% to 80% the in vitro differentiation of purified B cells from four of five patients with MGUS and from one of five patients with WM as assessed by the IgM content of day 7 culture supernatants. We next determined whether this effect could be related to an inhibition of IL- 6 secretion by cultured B cells and/or a downregulation of the IL-6 receptor (IL-6R), which was constitutively expressed on patients' blood B cells. A 50% to 100% (mean, 80%) inhibition of IL-6 production was found in seven of 10 patients (five with MGUS and two with WM). The IL- 6R was no more detectable on cells from patients with MGUS after 2 days of treatment with RA and slightly downregulated in patients with WM. It was of interest that B cells susceptible to the action of RA belonged mostly to patients with IgM MGUS, which reinforces our previous data showing distinct requirements for IL-6-dependent differentiation of blood B cells from patients with VM or IgM MGUS.

scholarly journals Atorvastatin-Medicated Dentifrice Significantly Inhibits CD4+ T Cell Proliferation: In vitro Pilot Study

2017 ◽  
Vol 35 (2) ◽  
pp. 394-402
Author(s):  
David R Rosenberg ◽  
Jeremy R Kernitsky ◽  
Catherine X Andrade ◽  
Valeria Ramirez ◽  
Deborah Violant ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
Pilot Study ◽  
Cd4 T Cell ◽  
T Cell Proliferation ◽  
Proliferation In Vitro

HUMAN PANCREATIC B-CELL PROLIFERATION IN VITRO AND IN VIVO

1996 ◽  
Vol 24 (4) ◽  
pp. 511S-511S
Author(s):  
B. Tyrberg ◽  
D.L. Eizirik ◽  
C. Hellerström ◽  
D.G. Pipeleers ◽  
A. Andersson
Keyword(s):  
Cell Proliferation ◽  
B Cell ◽  
Pancreatic B Cell ◽  
Proliferation In Vitro

Growth hormone inhibits normal B-cell differentiation and neutrophils' chemotaxis in vitro

1994 ◽  
Vol 16 (8) ◽  
pp. 667-673 ◽  
Author(s):  
M.Cecilia Fornari ◽  
Mariano P. Scolnik ◽  
M.Fernanda Palacios ◽  
Alberto D. Intebi ◽  
Roberto A. Diez
Keyword(s):  
Growth Hormone ◽  
Cell Differentiation ◽  
B Cell ◽  
B Cell Differentiation
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