PERSISTENT FUSION PORES BUT TRANSIENT FUSION IN ALVEOLAR TYPE II CELLS

2000 ◽  
Vol 24 (11) ◽  
pp. 803-807 ◽  
Author(s):  
P Dietl
Keyword(s):  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells ◽  
Fusion Pores

scholarly journals Fusion pore expansion is a slow, discontinuous, and Ca2+-dependent process regulating secretion from alveolar type II cells

2001 ◽  
Vol 155 (2) ◽  
pp. 279-290 ◽  
Author(s):  
Thomas Haller ◽  
Paul Dietl ◽  
Kristian Pfaller ◽  
Manfred Frick ◽  
Norbert Mair ◽  
...  
Keyword(s):  
Pore Diameter ◽  
Fusion Pore ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells ◽  
Intact Cells ◽  
Fusion Pores ◽  
Pyridinium Dibromide ◽  
Pore Expansion

In alveolar type II cells, the release of surfactant is considerably delayed after the formation of exocytotic fusion pores, suggesting that content dispersal may be limited by fusion pore diameter and subject to regulation at a postfusion level. To address this issue, we used confocal FRAP and N-(3-triethylammoniumpropyl)-4-(4-[dibutylamino]styryl) pyridinium dibromide (FM 1-43), a dye yielding intense localized fluorescence of surfactant when entering the vesicle lumen through the fusion pore (Haller, T., J. Ortmayr, F. Friedrich, H. Volkl, and P. Dietl. 1998. Proc. Natl. Acad. Sci. USA. 95:1579–1584). Thus, we have been able to monitor the dynamics of individual fusion pores up to hours in intact cells, and to calculate pore diameters using a diffusion model derived from Fick's law. After formation, fusion pores were arrested in a state impeding the release of vesicle contents, and expanded at irregular times thereafter. The expansion rate of initial pores and the probability of late expansions were increased by elevation of the cytoplasmic Ca2+ concentration. Consistently, content release correlated with the occurrence of Ca2+ oscillations in ATP-treated cells, and expanded fusion pores were detectable by EM. This study supports a new concept in exocytosis, implicating fusion pores in the regulation of content release for extended periods after initial formation.

Giant Lamellar Bodies in Alveolar Type II Cells of Rats Exposed to a Low Concentration of Ozone

Respiration ◽  
1984 ◽  
Vol 46 (3) ◽  
pp. 303-309 ◽  
Author(s):  
Sanae Shimura ◽  
Shinsaku Maeda ◽  
Tamotsu Takismima
Keyword(s):  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Lamellar Bodies ◽  
Alveolar Type Ii Cells ◽  
Low Concentration

Protection against paraquat-induced injury by exogenous GSH in pulmonary alveolar type II cells

1986 ◽  
Vol 35 (24) ◽  
pp. 4537-4542 ◽  
Author(s):  
Tory M. Hagen ◽  
Lou Ann Brown ◽  
Dean P. Jones
Keyword(s):  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells

XANTHINE OXIDASE (XO) STRESS OF ALVEOLAR TYPE II CELLS (T2C) MANIFESTS AS SYNTHETIC IMPAIRMENT AND COMPROMISED DNA INTEGRITY.

1995 ◽  
Vol 23 (Supplement) ◽  
pp. A215
Author(s):  
Clark Williams ◽  
Wayne Ciesielski ◽  
Michelle Johns ◽  
Jerry Zimmerman
Keyword(s):  
Xanthine Oxidase ◽  
Dna Integrity ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells
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