CELL POSITION-DEPENDENT RECIPROCAL FEEDBACK REGULATION OF TYPE I COLLAGEN GENE EXPRESSION IN CULTURED HUMAN SKIN FIBROBLASTS

1998 ◽  
Vol 22 (3) ◽  
pp. 185-191 ◽  
Author(s):  
R HATA
Keyword(s):  
Gene Expression ◽  
Human Skin ◽  
Type I Collagen ◽  
Feedback Regulation ◽  
Skin Fibroblasts ◽  
Type I ◽  
Collagen Gene ◽  
Human Skin Fibroblasts ◽  
Collagen Gene Expression ◽  
Cell Position

scholarly journals Interleukin-1β prevents the stimulatory effect of transforming growth factor-β on collagen gene expression in human skin fibroblasts

1990 ◽  
Vol 271 (3) ◽  
pp. 827-830 ◽  
Author(s):  
J Heino ◽  
T Heinonen
Keyword(s):  
Gene Expression ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Collagen Gene ◽  
Mrna Levels ◽  
Tgf Beta ◽  
Human Skin Fibroblasts ◽  
Collagen Mrna ◽  
Collagen Gene Expression ◽  
Beta 2

Transforming growth factors beta 1 and beta 2 (TGF-beta 1 and TGF-beta 2) are well-characterized strong inducers of collagen gene expression. A 100 pM concentration of TGF-beta 1 or TGF-beta 2 increases pro alpha 1(I) collagen mRNA levels in human skin fibroblasts 6.6-fold and 7.0-fold respectively, and also increases the accumulation of procollagens in the cell culture medium. Interleukin-1 beta (IL-1 beta) is an inflammatory mediator which also regulates connective tissue metabolism. A small concentration of IL-1 beta (0.01-1.0 unit/ml) slightly increases pro alpha 1(I) collagen mRNA levels (2.2-fold). Here we provide evidence that IL-1 beta prevents the stimulatory effect of TGFs-beta on collagen synthesis in human skin fibroblasts. An IL-1 beta concentration of 1 unit/ml is enough to keep pro alpha 1(I) collagen mRNA levels at control values in cells stimulated by 100 pM-TGF-beta 1. Thus the results indicate that IL-1 beta inhibits collagen synthesis in cells activated by TGFs-beta, whereas it does not significantly change or might even stimulate collagen gene expression in non-activated cells.

Tissue specificity of type I collagen gene expression is determined at both transcriptional and post-transcriptional levels

1984 ◽  
Vol 4 (9) ◽  
pp. 1843-1852
Author(s):  
R J Focht ◽  
S L Adams
Keyword(s):  
Gene Expression ◽  
Rna Structure ◽  
Type I Collagen ◽  
Translational Efficiency ◽  
Type I ◽  
Collagen Gene ◽  
Differentiated Cells ◽  
Collagen Gene Expression

We analyzed the control of type I collagen synthesis in four kinds of differentiated cells from chicken embryos which synthesize very different amounts of the protein. Tendon, skin, and smooth muscle cells were found to have identical amounts of type I collagen RNAs; however, the RNAs had inherently different translatabilities, which were observed both in vivo and in vitro. Chondrocytes also had substantial amounts of type I collagen RNAs, even though they directed no detectable synthesis of the protein either in vivo or in vitro. Type I collagen RNAs in chondrocytes display altered electrophoretic mobilities, suggesting that in these cells the reduction in translational efficiency may be mediated in part by changes in the RNA structure. These data indicate that control of type I collagen gene expression is a complex process which is exerted at both transcriptional and post-transcriptional levels.

scholarly journals Stimulation of Type I Collagen Transcription in Human Skin Fibroblasts by TGF-β: Involvement of Smad 3

1999 ◽  
Vol 112 (1) ◽  
pp. 49-57 ◽  
Author(s):  
Shu-Jen Chen ◽  
Weihua Yuan ◽  
Yasuji Mori ◽  
Anait Levenson ◽  
John Varga ◽  
...  
Keyword(s):  
Human Skin ◽  
Type I Collagen ◽  
Skin Fibroblasts ◽  
Type I ◽  
Human Skin Fibroblasts ◽  
Smad 3 ◽  
Stimulation Of
Sign in / Sign up

Export Citation Format

Share Document