INFLUENCE OF SUCCESSIVE AND COMBINED ULTRAVIOLET A AND B IRRADIATIONS ON MATRIX METALLOELASTASES PRODUCED BY HUMAN DERMAL FIBROBLASTS IN CULTURE

1997 ◽  
Vol 21 (6) ◽  
pp. 347-352 ◽  
Author(s):  
C KUT
Keyword(s):  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Ultraviolet A

scholarly journals Senescence of Human Fibroblasts after Psoralen Photoactivation Is Mediated by ATR Kinase and Persistent DNA Damage Foci at Telomeres

2006 ◽  
Vol 17 (4) ◽  
pp. 1758-1767 ◽  
Author(s):  
Miriam Grosse Hovest ◽  
Nicole Brüggenolte ◽  
Kijawasch Shah Hosseini ◽  
Thomas Krieg ◽  
Gernot Herrmann
Keyword(s):  
Dna Damage ◽  
Human Fibroblasts ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Ultraviolet A ◽  
Cross Link ◽  
Telomeric Dna ◽  
Histone H2ax ◽  
Senescent Phenotype ◽  
Atr Kinase

Cellular senescence is a phenotype that is likely linked with aging. Recent concepts view different forms of senescence as permanently maintained DNA damage responses partially characterized by the presence of senescence-associated DNA damage foci at dysfunctional telomeres. Irradiation of primary human dermal fibroblasts with the photosensitizer 8-methoxypsoralen and ultraviolet A radiation (PUVA) induces senescence. In the present study, we demonstrate that senescence after PUVA depends on DNA interstrand cross-link (ICL) formation that activates ATR kinase. ATR is necessary for the manifestation and maintenance of the senescent phenotype, because depletion of ATR expression before PUVA prevents induction of senescence, and reduction of ATR expression in PUVA-senesced fibroblasts releases cells from growth arrest. We find an ATR-dependent phosphorylation of the histone H2AX (γ-H2AX). After PUVA, ATR and γ-H2AX colocalize in multiple nuclear foci. After several days, only few predominantly telomere-localized foci persist and telomeric DNA can be coimmunoprecipitated with ATR from PUVA-senesced fibroblasts. We thus identify ATR as a novel mediator of telomere-dependent senescence in response to ICL induced by photoactivated psoralens.

scholarly journals Ultraviolet A Enhances Cathepsin L Expression and Activity via JNK Pathway in Human Dermal Fibroblasts

2016 ◽  
Vol 129 (23) ◽  
pp. 2853-2860 ◽  
Author(s):  
Qing-Fang Xu ◽  
Yue Zheng ◽  
Jian Chen ◽  
Xin-Ya Xu ◽  
Zi-Jian Gong ◽  
...  
Keyword(s):  
Cathepsin L ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Ultraviolet A ◽  
Jnk Pathway ◽  
Expression And Activity

Singlet oxygen is an early intermediate in cytokine-dependent ultraviolet-A induction of interstitial collagenase in human dermal fibroblasts in vitro

FEBS Letters ◽  
1997 ◽  
Vol 413 (2) ◽  
pp. 239-242 ◽  
Author(s):  
Meinhard Wlaschek ◽  
Jutta Wenk ◽  
Peter Brenneisen ◽  
Karlis Briviba ◽  
Agatha Schwarz ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Ultraviolet A ◽  
Interstitial Collagenase

scholarly journals Ultraviolet A Induces Endoplasmic Reticulum Stress Response in Human Dermal Fibroblasts

2012 ◽  
Vol 37 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Ryota Komori ◽  
Mai Taniguchi ◽  
Yoshiaki Ichikawa ◽  
Aya Uemura ◽  
Masaya Oku ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Stress Response ◽  
Endoplasmic Reticulum Stress ◽  
Dermal Fibroblasts ◽  
Endoplasmic Reticulum Stress Response ◽  
Human Dermal Fibroblasts ◽  
Ultraviolet A

scholarly journals Antioxidative and antiphotoaging activities of neferine upon UV-A irradiation in human dermal fibroblasts

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Abidullah khan ◽  
Hongliang Bai ◽  
Maoguo Shu ◽  
Mingxia Chen ◽  
Amin Khan ◽  
...  
Keyword(s):  
Deleterious Effect ◽  
Dermal Fibroblasts ◽  
The Other ◽  
Oxygen Species ◽  
Human Dermal Fibroblasts ◽  
Ultraviolet A ◽  
Enzymatic Antioxidant ◽  
Matrix Metalloproteinase 1 ◽  
Prevention And Treatment ◽  
Skin Photoaging

Our daily exposure to ultraviolet radiation (UVR) results in the production of reactive oxygen species (ROS), lipids, proteins and DNA damage and alteration in fibroblast structure, thus contributing to skin photoaging. For this reason, the use of natural bioactive compounds with antioxidant activity could be a strategic tool to overcome ultraviolet A (UV-A) induced deleterious effect. Neferine is an alkaloid extract from the seed embryos of lotus (Nelumbo nucifera Gaertn). In the present study, we report the protective effect of neferine against UV-A induced oxidative stress and photoaging in human dermal fibroblasts (HDFs). HDFs subjected to UV-A irradiation showed increased production of ROS and malondialdehyde (MDA). Furthermore, it depleted the cellular enzymatic antioxidant superoxide dismutase (SOD) and non-enzymatic antioxidant glutathione peroxidase (GPx). On the other hand, HDFs treated with neferine followed by UV-A irradiation reversed the process, reduced the ROS and lipid peroxidation and restored the antioxidants pool. Moreover, neferine treatment significantly inhibited UV-A induced matrix metalloproteinase-1 (MMP-1) expression in HDFs. Remarkable morphological and ultrastructural alterations observed in HDFs upon UV-A irradiation, were also reduced with neferine treatment. Taken together, our results suggest that neferine has strong antioxidative and photoprotective properties and thus may be a potential agent for the prevention and treatment of UV-A mediated skin photoaging.

Sign in / Sign up

Export Citation Format

Share Document