CELL SURFACE LOCALIZATION OF THE 60 kDa HEAT SHOCK CHAPERONIN PROTEIN (hsp60) IN MAMMALIAN CELLS

1997 ◽  
Vol 21 (5) ◽  
pp. 315-320 ◽  
Author(s):  
B SOLTYS
Keyword(s):  
Heat Shock ◽  
Cell Surface ◽  
Mammalian Cells ◽  
Surface Localization ◽  
Cell Surface Localization

scholarly journals Native adiponectin in serum binds to mammalian cells expressing T-cadherin, but not AdipoRs or calreticulin

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Shunbun Kita ◽  
Shiro Fukuda ◽  
Norikazu Maeda ◽  
Iichiro Shimomura
Keyword(s):  
Cell Surface ◽  
Human Serum ◽  
Cho Cells ◽  
Mammalian Cells ◽  
Hek293 Cells ◽  
C2c12 Myotubes ◽  
Binding Partner ◽  
Surface Localization ◽  
Significant Attenuation ◽  
Cell Surface Localization

Adiponectin is an adipocyte-derived atypically abundant circulating factor that protects various organs and tissues through its receptors, AdipoRs, calreticulin, and T-cadherin. To identify the major binding partner of circulating native adiponectin, we expressed these receptors on the surface of HEK293 cells. Adiponectin, either that in mouse or human serum, purified from serum, or produced by mammalian cells, bound to cells expressing T-cadherin, but not to those expressing AdipoR1 or calreticulin. The stable introduction of T-cadherin and AdipoR1 into CHO cells resulted in the cell surface localization of these receptors. Native adiponectin in serum bound to cells expressing T-cadherin, not to those expressing AdipoR1. The knockdown of T-cadherin, but not AdipoRs resulted in the significant attenuation of native adiponectin binding to C2C12 myotubes. Therefore, native adiponectin binding depended on the amount of T-cadherin expressed in HEK293 cells, CHO cells, and C2C12 myotubes. Collectively, our mammalian cell-based studies suggest that T-cadherin is the major binding partner of native adiponectin in serum.

scholarly journals A novel motif in the proximal C-terminus of Pannexin 1 regulates cell surface localization

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Anna L. Epp ◽  
Sarah N. Ebert ◽  
Juan C. Sanchez-Arias ◽  
Leigh E. Wicki-Stordeur ◽  
Andrew K. J. Boyce ◽  
...  
Keyword(s):  
Cell Surface ◽  
Pannexin 1 ◽  
C Terminus ◽  
Surface Localization ◽  
Cell Surface Localization

scholarly journals Cell Surface Localization of Proteolysis of Human Endothelial Angiotensin I-converting Enzyme

1995 ◽  
Vol 270 (48) ◽  
pp. 28962-28969 ◽  
Author(s):  
Véronique Beldent ◽  
Annie Michaud ◽  
Christophe Bonnefoy ◽  
Marie-Thérèse Chauvet ◽  
Pierre Corvol
Keyword(s):  
Cell Surface ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Angiotensin I Converting Enzyme ◽  
Surface Localization ◽  
Cell Surface Localization
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