Early endocytotic steps in elicited macrophages: omega-shaped plasma membrane vesicles at their cell surface.

1995 ◽  
Vol 19 (6) ◽  
pp. 527-538 ◽  
Author(s):  
A Kiss
Keyword(s):  
Plasma Membrane ◽  
Cell Surface ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles

scholarly journals The lipoprotein lipase of white adipose tissue. Studies on the intracellular distribution of the adipocyte-associated enzyme

1986 ◽  
Vol 236 (3) ◽  
pp. 749-756 ◽  
Author(s):  
A A Al-Jafari ◽  
A Cryer
Keyword(s):  
Plasma Membrane ◽  
Cell Surface ◽  
Guinea Pig ◽  
Lipoprotein Lipase ◽  
Membrane Vesicles ◽  
Capillary Endothelium ◽  
Enzyme Analysis ◽  
Marker Enzyme ◽  
Igg Antibodies ◽  
Plasma Membrane Vesicles

The separation of rat epididymal adipocytes into plasma-membrane, mitochondrial, microsomal and cytosol fractions is described. The fractions, which were characterized by marker-enzyme analysis and electron-micrographic observation, from the cells of fed and 24 h-starved animals were used to prepare acetone/diethyl ether-dried powders for the measurement of lipoprotein lipase activities. The highest specific activities and proportion of recovered lipoprotein lipase activity were found in the plasma-membrane and microsomal fractions. The two fractions from the cells of fed rats showed similar activities and enrichments of the enzyme, these activities being higher than the plasma-membrane and lower than the microsomal activities recovered from the cells of starved animals. Chicken and guinea-pig anti-(rat lipoprotein lipase) sera were prepared, and an indirect labelled-second-antibody cellular immunoassay, using 125I-labelled rabbit anti-(chicken IgG) or 125I-labelled sheep anti-(guinea-pig IgG) antibodies respectively, for the detection of cell-surface enzyme was devised and optimized. The amount of immunodetectable cell-surface lipoprotein lipase was higher for cells isolated from fed animals than for cells from 24 h-starved animals, when either anti-(lipoprotein lipase) serum was used in the assay. The amount of immunodetectable cell-surface lipoprotein lipase fell further when starvation was extended to 48 h. The lipoprotein lipase of plasma-membrane vesicles was shown to be a patent activity and to be immunodetectable in a modification of the cellular immunoassay. Although the functional significance of the adipocyte surface lipoprotein lipase is not known, the possibility of it forming a pool of enzyme en route to the capillary endothelium is advanced.

Calcium efflux of plasma membrane vesicles exposed to ELF magnetic fields-test of a nuclear magnetic resonance interaction model

2012 ◽  
Vol 33 (7) ◽  
pp. 535-542 ◽  
Author(s):  
Wenjun J. Sun ◽  
Mehri Kaviani Mogadam ◽  
Marianne Sommarin ◽  
Henrietta Nittby ◽  
Leif G. Salford ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Plasma Membrane ◽  
Magnetic Resonance ◽  
Magnetic Fields ◽  
Interaction Model ◽  
Membrane Vesicles ◽  
Resonance Interaction ◽  
Calcium Efflux ◽  
Plasma Membrane Vesicles ◽  
Nuclear Magnetic

Pore-Spanning Plasma Membranes Derived from Giant Plasma Membrane Vesicles

2021 ◽  
Author(s):  
Nikolas K. Teiwes ◽  
Ingo Mey ◽  
Phila C. Baumann ◽  
Lena Strieker ◽  
Ulla Unkelbach ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Plasma Membranes ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles
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