Altered Functional Responses with Preserved Morphology of Gonadotrophic Cells in Congenitally Athymic Mice

2001 ◽  
Vol 15 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Rodolfo G. Goya ◽  
Gloria M. Cónsole ◽  
Yolanda E. Sosa ◽  
César L. Gómez Dumm ◽  
Mireille Dardenne
Keyword(s):  
Athymic Mice ◽  
Functional Responses ◽  
Gonadotrophic Cells

Spontaneous Cataract in Nude Athymic Mice

1977 ◽  
Vol 35 ◽  
pp. 512-513
Author(s):  
Ronald H. Bradley ◽  
R. S. Berk ◽  
L. D. Hazlett
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nude Mouse ◽  
Cellular Immunity ◽  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Athymic Mice ◽  
Transmission Microscopy ◽  
Mouse Lens ◽  
Scanning Electron

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

Growth and hormonal content of human fetal pancreas passaged in athymic mice

Diabetes ◽  
1986 ◽  
Vol 35 (4) ◽  
pp. 464-469 ◽  
Author(s):  
B. E. Tuch ◽  
S. Grigoriou ◽  
J. R. Turtle
Keyword(s):  
Athymic Mice ◽  
Fetal Pancreas ◽  
Human Fetal Pancreas ◽  
Hormonal Content

EXPERIMENTAL STUDY OF THE EFFICACY OF L-LYSINE-A-OXIDASE TRICHODERMA CF. AUREOVIRIDE RIFAI ON MODELS OF XENOGRAFTS OF HUMAN TUMORS IN ATHYMIC MICE AND EVALUATION OF SYNERGISM WITH CISPLATIN OR TOPOISOMERASE INHIBITORS

2017 ◽  
Vol 63 (3) ◽  
pp. 497-505
Author(s):  
Vadim Pokrovskiy ◽  
Yelena Lukasheva ◽  
Nikolay Chernov ◽  
Yelena Treshchalina
Keyword(s):  
Experimental Study ◽  
Life Expectancy ◽  
Therapeutic Benefit ◽  
Human Tumors ◽  
Lymphocytic Leukemia ◽  
Antitumor Drugs ◽  
Athymic Mice ◽  
Lewis Lung ◽  
Topoisomerase Inhibitors ◽  
Inhibition Of Growth

The effectiveness of L-lysine-alpha oxidase Trichoderma cf. Aureoviride Rifai BKMF-4268D (LO) on models of subcutaneous xenografts of human tumors in athymic mice as well as the effectiveness of combination therapy with known antitumor drugs: cisplatin, irinotecan, etoposide on models of P388 lymphocytic leukemia, Lewis lung (LLC) and B16 melanoma was evaluated. The intraperitoneal injection of LO in a discrete regime at doses of 150-75-75-75-75 E/kg demonstrated inhibition of growth of all studied xenografts of human tumors in athymic mice. The combination of irinotecan+LO on the LLC model gave a significant summative therapeutic benefit with an increase in mouse life expectancy up to 35%. Cisplatin and LO realized a significant (p

Tyrosine kinase-dependent phosphatidylinositol turnover and functional responses in the FcεR1 signalling pathway

1991 ◽  
Vol 179 (1) ◽  
pp. 551-557 ◽  
Author(s):  
Grace G. Deanin ◽  
A.Marina Martinez ◽  
Janet R. Pfeiffer ◽  
Mary E. Gardner ◽  
Janet M. Oliver
Keyword(s):  
Tyrosine Kinase ◽  
Signalling Pathway ◽  
Functional Responses ◽  
Phosphatidylinositol Turnover

Expression of P2X7purinoceptors on human lymphocytes and monocytes: evidence for nonfunctional P2X7receptors

2000 ◽  
Vol 279 (4) ◽  
pp. C1189-C1197 ◽  
Author(s):  
B. J. Gu ◽  
W. Y. Zhang ◽  
L. J. Bendall ◽  
I. P. Chessell ◽  
G. N. Buell ◽  
...  
Keyword(s):  
B Lymphocytes ◽  
Adhesion Molecule ◽  
Human Lymphocytes ◽  
Normal Subjects ◽  
Cell Types ◽  
Surface Expression ◽  
Lymphocytic Leukemia ◽  
Functional Responses ◽  
Intracellular Expression ◽  
Selective Channel

Lymphocytes from normal subjects and patients with B-chronic lymphocytic leukemia (B-CLL) show functional responses to extracellular ATP characteristic of the P2X7receptor (previously termed P2Z). These responses include opening of a cation-selective channel/pore that allows entry of the fluorescent dye ethidium and activation of a membrane metalloprotease that sheds the adhesion molecule L-selectin. The surface expression of P2X7receptors was measured in normal leucocytes, platelets, and B-CLL lymphocytes and correlated with their functional responses. Monocytes showed four- to fivefold greater expression of P2X7than B, T, and NK lymphocytes, whereas P2X7expression on neutrophils and platelets was weak. All cell types demonstrated abundant intracellular expression of this receptor. All 12 subjects with B-CLL expressed lymphocyte P2X7at about the same level as B lymphocytes from normal subjects. P2X7function, measured by ATP-induced uptake of ethidium, correlated closely with surface expression of this receptor in normal and B-CLL lymphocytes and monocytes ( n = 47, r = 0.70; P< 0.0001). However, in three patients the ATP-induced uptake of ethidium into the malignant B lymphocytes was low or absent. The lack of P2X7function in these B lymphocytes was confirmed by the failure of ATP to induce Ba2+uptake into their lymphocytes. This lack of function of the P2X7receptor resulted in a failure of ATP-induced shedding of L-selectin, an adhesion molecule that directs the recirculation of lymphocytes from blood into the lymph node.

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