Physicochemical Methods for Predicting the Biological Potency of Recombinant Follicle Stimulating Hormone: An International Collaborative Study of Isoelectric Focusing and Capillary Zone Electrophoresis

Biologicals ◽  
2002 ◽  
Vol 30 (3) ◽  
pp. 217-234 ◽  
Author(s):  
Patrick L. Storring ◽  
Rose E. Gaines Das ◽  
J.W.M. Mulders ◽  
Marlies Halder
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Isoelectric Focusing ◽  
Follicle Stimulating Hormone ◽  
Collaborative Study ◽  
Zone Electrophoresis ◽  
Biological Potency ◽  
Capillary Zone ◽  
Physicochemical Methods ◽  
International Collaborative ◽  
International Collaborative Study

A Capillary Zone Electrophoresis Method for Analysis of Glycosylated Recombinant Human Follicle-Stimulating Hormone

2019 ◽  
Vol 35 (4) ◽  
pp. 55-64
Author(s):  
M.E. Sushkin ◽  
A.S. Simbirtsev
Keyword(s):  
Sodium Chloride ◽  
Capillary Zone Electrophoresis ◽  
Protein Separation ◽  
Follicle Stimulating Hormone ◽  
Background Electrolyte ◽  
Sialic Acids ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone ◽  
Stimulating Hormone

Conditions for analyzing sialylated isoforms of recombinant follicle-stimulating hormone (FSH) by the method of capillary zone electrophoresis have been selected. Follicle-stimulating hormone is a glycosylated heterodimer with the large number of sialic acids that affect its basic biological and physicochemical properties. The optimum protein separation into 6 isoforms was achieved using as a background electrolyte of a solution with pH 4.3 containing 0.01 M trycin, 0.01 sodium chloride, 7 M urea and 2.5 mM 1,4-diaminobutane. follicle-stimulating hormone isoforms, capillary zone electrophoresis, sialic acids, follicle-stimulating hormone.

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