Biological Characterization of a Nucleopolyhedrovirus of Spodoptera litura (Lepidoptera: Noctuidae) Isolated from the Philippines

2001 ◽  
Vol 20 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Barbara A Laviña ◽  
Leodegario E Padua ◽  
Fu Quan Wu ◽  
Noriko Shirata ◽  
Motoko Ikeda ◽  
...  
Keyword(s):  
Spodoptera Litura ◽  
The Philippines ◽  
Biological Characterization ◽  
Lepidoptera Noctuidae

scholarly journals Survey and identification of entomopathogenic nematodes in the province of Cotabato, Philippines, for biocontrol potential against the tobacco cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Cynthia Ann Dichusa ◽  
Rolando Ramos ◽  
Sitaram Aryal ◽  
Neil Pep Dave Sumaya ◽  
Nanette Hope Sumaya
Keyword(s):  
Spodoptera Litura ◽  
Entomopathogenic Nematodes ◽  
Insect Pest ◽  
First Record ◽  
The Philippines ◽  
Morphological Data ◽  
Infective Juvenile ◽  
Tobacco Cutworm ◽  
Biocontrol Potential ◽  
Lepidoptera Noctuidae

Abstract Background The tobacco cutworm, Spodoptera litura [Fab.] (Lepidoptera: Noctuidae), is a devastating insect pest of several crops. Entomopathogenic nematodes (EPNs) of the families Heterorhabditidae and Steinernematidae are used as an alternative control measure in lieu of the hazardous synthetic chemical applications. Results A survey of naturally occurring EPNs was conducted across the province of Cotabato, Philippines, covering a total of 5 municipalities with 25 villages. Galleria-baiting technique was employed to recover nematodes from peanut and grassland soils. Out of 50 soil samples collected, only 5 samples harbored nematodes, indicating a recovery of 10%. Preliminary morphological data identified only one EPN under the genera Heterorhabditis (1 isolate), whereas 4 were facultative necromenic nematodes from the genera Metarhabditis (2 isolates) and Oscheius (2 isolates). Analysis of D2D3 segments of the 28S rDNA confirmed high sequence similarity to Heterorhabditis indica, Metarhabditis rainai, Oscheius insectivora, and Oscheius sp. This is the first record of H. indica and M. rainai in the entire region, whereas the first record for Oscheius spp. in the Philippines. Furthermore, the biocontrol potential of the local H. indica infective juvenile (IJ) populations (PIGCD1) isolated from peanut was assessed against the tobacco cutworm, S. litura, under laboratory conditions. The mean percentage mortality caused by H. indica on S. litura at 7 different concentrations ranged from 0-100% at 24 h post inoculation. The lethal concentration (LC50) required to kill 50% of the S. litura larvae population with H. indica was 7.13±1 (IJs/larva). Conclusions The use of Galleria-baiting method is a convenient approach to detect EPNs including other facultative necromenic nematodes from the soils. Obtained data indicated that the local H. indica isolate can be a promising alternative measure to suppress the economically important insect pest, S. litura, and this may provide significant outlook to establish the biocontrol program in the country.

Genetic and Biological Characterization of Four Nucleopolyhedrovirus Isolates Collected in Mexico for the Control of Spodoptera exigua (Lepidoptera: Noctuidae)

2017 ◽  
Vol 110 (4) ◽  
pp. 1465-1475 ◽  
Author(s):  
N. Zamora-Avilés ◽  
R. Murillo ◽  
R. Lasa ◽  
S. Pineda ◽  
J. I. Figueroa ◽  
...  
Keyword(s):  
Spodoptera Exigua ◽  
Biological Characterization ◽  
Lepidoptera Noctuidae
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