Extracellular pH Modulates Helicobacter pylori-Induced Vacuolation and VacA Toxin Internalization in Human Gastric Epithelial Cells

2002 ◽  
Vol 292 (1) ◽  
pp. 167-174 ◽  
Author(s):  
Vittorio Ricci ◽  
Patrizia Sommi ◽  
Roberto Fiocca ◽  
Vittorio Necchi ◽  
Marco Romano ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Extracellular Ph ◽  
Gastric Epithelial Cells

Effect of Helicobacter pylori on Cell Gap Junction Ultrastructure of Gastric Epithelial Cells

2009 ◽  
Vol 2009 (6) ◽  
pp. 722-728
Author(s):  
Can-Xia XU ◽  
Yan JIA ◽  
Wen-Bin YANG ◽  
Hui-Fang ZOU ◽  
Fen WANG ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Gap Junction ◽  
Gastric Epithelial Cells

M1959 Helicobacter pylori cagA Tyrosine Phosphorylation Triggers the Interleukin-11/STAT3 Pathway in Gastric Epithelial Cells

2010 ◽  
Vol 138 (5) ◽  
pp. S-448
Author(s):  
Kai Syin Lee ◽  
Anastasia Kalantzis ◽  
Naoko Murata-Kamiya ◽  
Masanori Hatakeyama ◽  
Andrew S. Giraud ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Tyrosine Phosphorylation ◽  
Gastric Epithelial Cells ◽  
Stat3 Pathway ◽  
Interleukin 11

scholarly journals Effects ofHelicobacter pyloriand Heat Shock Protein 70 on the Proliferation of Human Gastric Epithelial Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Liping Tao ◽  
Hai Zou ◽  
Zhimin Huang
Keyword(s):  
Helicobacter Pylori ◽  
Heat Shock ◽  
Epithelial Cells ◽  
Heat Shock Protein ◽  
Mtt Assay ◽  
Heat Shock Protein 70 ◽  
Hsp70 Expression ◽  
Gastric Epithelial Cells ◽  
Ags Cells ◽  
H Pylori

Infection ofHelicobacter pylori (H. pylori)changed the proliferation of gastric epithelial cells and decreased the expression of heat shock protein 70 (HSP70). However, the effects ofH. pylorion the proliferation of gastric epithelial cells and the roles of HSP70 during the progress need further investigation.Objective.To investigate the effects ofHelicobacter pylori (H. pylori)and heat shock protein 70 (HSP70) on the proliferation of human gastric epithelial cells.Methods. H. pyloriand a human gastric epithelial cell line (AGS) were cocultured. The proliferation of AGS cells was quantitated by an MTT assay, and the expression of HSP70 in AGS cells was detected by Western blotting. HSP70 expression in AGS cells was silenced by small interfering RNA (siRNA) to investigate the role of HSP70. ThesiRNA-treated AGS cells were cocultured withH. pyloriand cell proliferation was measured by an MTT assay.Results.The proliferation of AGS cells was accelerated by coculturing withH. pylorifor 4 and 8 h, but was suppressed at 24 and 48 h. HSP70 expression was decreased in AGS cells infected byH. pylorifor 48 h. The proliferation in HSP70-silenced AGS cells was inhibited after coculturing withH. pylorifor 24 and 48 h compared with the control group.Conclusions.Coculture ofH. pylorialtered the proliferation of gastric epithelial cells and decreased HSP70 expression. HSP70 knockdown supplemented the inhibitory effect ofH. pylorion proliferation of epithelial cells. These results indicate that the effects ofH. pylorion the proliferation of gastric epithelial cells at least partially depend on the decreased expression of HSP70 induced by the bacterium.

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