Cloned and Expressed Fungal phyA Gene in Alfalfa Produces a Stable Phytase

2002 ◽  
Vol 290 (4) ◽  
pp. 1343-1348 ◽  
Author(s):  
Abul H.J Ullah ◽  
Kandan Sethumadhavan ◽  
Edward J Mullaney ◽  
Thomas Ziegelhoffer ◽  
Sandra Austin-Phillips
Keyword(s):  
Phya Gene

Biolistic transformation of cotton (Gossypium hirsutum L.) with the phyA gene from Aspergillus ficuum

2011 ◽  
Vol 106 (2) ◽  
pp. 207-214 ◽  
Author(s):  
J. F. Liu ◽  
X. F. Wang ◽  
Q. L. Li ◽  
X. Li ◽  
G. Y. Zhang ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Biolistic Transformation ◽  
Aspergillus Ficuum ◽  
Gossypium Hirsutum L ◽  
Phya Gene

Cloning, expression, and characterization of phyA gene from Ipomoea batatas

2015 ◽  
Vol 62 (1) ◽  
pp. 109-115
Author(s):  
J. X. Wang ◽  
Y. S. Jiang ◽  
Y. Yan ◽  
X. Tao ◽  
Y. Z. Zhang
Keyword(s):  
Ipomoea Batatas ◽  
Phya Gene

phyA Gene Promoter Analysis

1991 ◽  
pp. 499-508
Author(s):  
Peter H. Quail ◽  
Wesley B. Bruce ◽  
Katayoon Dehesh ◽  
Jacqueline Dulson
Keyword(s):  
Promoter Analysis ◽  
Gene Promoter ◽  
Phya Gene

scholarly journals A Dominant Mutation in the Pea PHYA Gene Confers Enhanced Responses to Light and Impairs the Light-Dependent Degradation of Phytochrome A

2004 ◽  
Vol 135 (4) ◽  
pp. 2186-2195 ◽  
Author(s):  
James L. Weller ◽  
Shona L. Batge ◽  
Jennifer J. Smith ◽  
L. Huub J. Kerckhoffs ◽  
Vitaly A. Sineshchekov ◽  
...  
Keyword(s):  
Phytochrome A ◽  
Dominant Mutation ◽  
Phya Gene

scholarly journals Heterologous phytase expression in the food filamentous fungus Aspergillus oryzae using the added rice husk cultivation model

2020 ◽  
Vol 42 (2) ◽  
Author(s):  
Thai Hanh Dung ◽  
Tran Van Tuan
Keyword(s):  
Aspergillus Oryzae ◽  
Filamentous Fungus ◽  
Culture Media ◽  
Phytase Activity ◽  
Fungal Mycelium ◽  
Rice Husks ◽  
Recombinant Enzymes ◽  
Gene Encoding ◽  
Phya Gene ◽  
Transgenic Strains

Aspergillus oryzae, a safe filamentous fungus, is widely used in food and enzyme production. In this study, we examined a cultivation model using rice husks as carrier to assess the capacity of recombinant protein production in A. oryzae. The model was first tested with the A. oryzae strain expressing the DsRed reporter gene. Expression of DsRed was easily detected by the pink color of the fungal mycelium on culture media and under a fluorescence microscope. The model was then evaluated with the phyA gene encoding a phytase from the fungus Aspergillus fumigatus. The phyA expression cassette regulated by the amyB promoter was permanently integrated into the genome of A. oryzae via Agrobacterium tumefaciens-mediated transformation with the pyrG nutritional marker. Results showed that A. oryzae transgenic strains carrying 2−3 copies of the phyA gene in their genomes exhibited a significant increase in phytase activity on agar medium supplemented with phytate. With rice husks added, these transgenic strains could secrete the recombinant phytase into the culture and phytase activity of the crude enzyme solution increased by 4.3 times compared to the unstransgenic A. oryzae. The established cultivation model and the transgenic approach in this study represent a potential for being used in production of secreted recombinant enzymes for animal feeds. 

Codon Optimization and Expression of phyA Gene in Soybean (Glycine max Merr.)

2016 ◽  
Vol 42 (12) ◽  
pp. 1798
Author(s):  
Ying-Ying KOU ◽  
Ying-Jin SONG ◽  
Shao-Hui YANG ◽  
Jie-Hua WANG
Keyword(s):  
Glycine Max ◽  
Codon Optimization ◽  
Phya Gene
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