Intravenous Delivery of Naked Plasmid DNA for in Vivo Cytokine Expression

2001 ◽  
Vol 289 (5) ◽  
pp. 1088-1092 ◽  
Author(s):  
Jingjing Jiang ◽  
Eiji Yamato ◽  
Jun-ichi Miyazaki
Keyword(s):  
Plasmid Dna ◽  
Cytokine Expression ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

In vivo expression and antitumor activity of p53 gene transfer with naked plasmid DNA in an ovarian cancer xenograft model in nude mice

2006 ◽  
Vol 32 (5) ◽  
pp. 449-453 ◽  
Author(s):  
Pierre Collinet ◽  
Rodolphe Vereecque ◽  
Frédéric Sabban ◽  
Denis Vinatier ◽  
Eric Leblanc ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gene Transfer ◽  
Antitumor Activity ◽  
Nude Mice ◽  
Plasmid Dna ◽  
Xenograft Model ◽  
P53 Gene ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

scholarly journals Uptake, Biodistribution, and Time Course of Naked Plasmid DNA Trafficking After Intratumoral In Vivo Jet Injection

2006 ◽  
Vol 0 (0) ◽  
pp. 060801084750032
Author(s):  
W. Walther ◽  
T. Minow ◽  
R. Martin ◽  
I. Fichtner ◽  
P.M. Schlag ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Time Course ◽  
Jet Injection ◽  
Naked Plasmid ◽  
Dna Trafficking ◽  
Naked Plasmid Dna

scholarly journals Ultrasound-targeted microbubble destruction enhances naked plasmid DNA transfection in rabbit Achilles tendons in vivo

Gene Therapy ◽  
2011 ◽  
Vol 19 (7) ◽  
pp. 703-710 ◽  
Author(s):  
L Qiu ◽  
L Zhang ◽  
L Wang ◽  
Y Jiang ◽  
Y Luo ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Transfection ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

Expression of naked plasmids by cultured myotubes and entry of plasmids into T tubules and caveolae of mammalian skeletal muscle

1992 ◽  
Vol 103 (4) ◽  
pp. 1249-1259
Author(s):  
J.A. Wolff ◽  
M.E. Dowty ◽  
S. Jiao ◽  
G. Repetto ◽  
R.K. Berg ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Plasmid Dna ◽  
Cardiac Cells ◽  
Mammalian Skeletal Muscle ◽  
Cultured Myotubes ◽  
Naked Plasmid ◽  
T Tubules ◽  
Naked Plasmid Dna

Plasmid DNA or artificial mRNA injected intramuscularly into skeletal muscle via a 27 g needle expressed transgenes at relatively efficient levels in skeletal myofibers and cardiac cells. In the present study, several approaches were used to determine the mechanism of cellular uptake. After exposure of naked plasmid DNA, primary rat muscle cells in vitro expressed transgenes to a much greater extent than other types of immortalized or primary cells. In vivo light microscope studies showed that intramuscularly injected plasmid DNA was distributed throughout the muscle and was able to diffuse through the extracellular matrix, cross the external lamina, and enter myofibers. Electron microscope studies showed that colloidal gold conjugated to plasmid DNA traversed the external lamina and entered T tubules and caveolae, while gold complexed with polylysine, polyethylene glycol or polyglutamate primarily remained outside of the myofibers. The results indicate that it is highly unlikely that the plasmid DNA enters the myofiber simply by the needle grossly disrupting the sarcolemma. In addition, transient membrane disruptions do not appear to be responsible for the uptake of DNA. Furthermore, no evidence for endocytosis could be found. The possible uptake of plasmid DNA by some type of cell membrane transporter, in particular via potocytosis, is discussed.

Ultrasound Facilitates Transduction of Naked Plasmid DNA into Colon Carcinoma Cells in Vitro and in Vivo

2000 ◽  
Vol 11 (11) ◽  
pp. 1521-1528 ◽  
Author(s):  
Yoshinobu Manome ◽  
Motoyuki Nakamura ◽  
Tsuneya Ohno ◽  
Hiroshi Furuhata
Keyword(s):  
Colon Carcinoma ◽  
Plasmid Dna ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

scholarly journals Uptake, Biodistribution, and Time Course of Naked Plasmid DNA Trafficking After Intratumoral In Vivo Jet Injection

2006 ◽  
Vol 17 (6) ◽  
pp. 611-624 ◽  
Author(s):  
W. Walther ◽  
T. Minow ◽  
R. Martin ◽  
I. Fichtner ◽  
P.M. Schlag ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Time Course ◽  
Jet Injection ◽  
Naked Plasmid ◽  
Dna Trafficking ◽  
Naked Plasmid Dna

scholarly journals In vivo Site-Specific Transfection of Naked Plasmid DNA and siRNAs in Mice by Using a Tissue Suction Device

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e41319 ◽  
Author(s):  
Kazunori Shimizu ◽  
Shigeru Kawakami ◽  
Kouji Hayashi ◽  
Hideyuki Kinoshita ◽  
Koichiro Kuwahara ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Site Specific ◽  
Suction Device ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

scholarly journals In Vivo Transgene Expression in the Pancreas by the Intraductal Injection of Naked Plasmid DNA

2018 ◽  
Vol 107 (2) ◽  
pp. 647-653 ◽  
Author(s):  
Yuma Yamada ◽  
Mai Tabata ◽  
Jiro Abe ◽  
Masatoshi Nomura ◽  
Hideyoshi Harashima
Keyword(s):  
Plasmid Dna ◽  
Transgene Expression ◽  
Naked Plasmid ◽  
Intraductal Injection ◽  
Naked Plasmid Dna
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