Gene Cloning and Characterization of Alanine Racemases from Shigella dysenteriae, Shigella boydii, Shigella flexneri, and Shigella sonnei

2001 ◽  
Vol 288 (3) ◽  
pp. 676-684 ◽  
Author(s):  
Kumio Yokoigawa ◽  
Reiko Hirasawa ◽  
Hiroshi Ueno ◽  
Yoko Okubo ◽  
Seiichi Umesako ◽  
...  
Keyword(s):  
Gene Cloning ◽  
Shigella Flexneri ◽  
Shigella Sonnei ◽  
Shigella Dysenteriae ◽  
Shigella Boydii ◽  
Gene Cloning And Characterization

scholarly journals Serotipos y susceptibilidad antimicrobiana de Shigella aisladas en un instituto de salud pediátrico de Lima, Perú entre enero y julio 2013

2014 ◽  
Vol 25 (2) ◽  
pp. 73
Author(s):  
Carlos Baca ◽  
Lisette Yupanqui ◽  
Jhan Canales ◽  
María Luz Zamudio ◽  
María del Carmen Quispe ◽  
...  
Keyword(s):  
Shigella Flexneri ◽  
Shigella Sonnei ◽  
Shigella Dysenteriae ◽  
Shigella Boydii

Objetivos: Determinar la frecuencia de serogrupos y serotipos y el perfil de susceptibilidad antimicrobiana de Shigella sp., aisladas en un instituto pediátrico de Lima, Perú. Material y métodos: Se evaluaron 85 aislamientos de Shigella sp., identificados bioquímicamente y serológicamente a nivel de serogrupo y serotipo por el método de aglutinación en lámina. Los patrones de resistencia antibiótica se determinaron mediante el método de disco difusión en agar. Resultados: De los 85 aislamientos de Shigella sp., 53 (62,3%) correspondieron al serogrupo B (Shigella flexneri), 28 (32,9%) al grupo D (Shigella sonnei) y 4 (4,8%) al grupo C (Shigella boydii), ningún aislamiento correspondió al grupo A (Shigella dysenteriae). Respecto a los serotipos, en el grupo B, fueron 46% 1b, 36% 2a y 18% variante Y; en el grupo C fue C4 y en el grupo D todos fueron Fase I. La evaluación del perfil de susceptibilidad mostró que el 100% de las cepas fueron sensibles a aztreonam, ácido nalidíxico y ciprofloxacina; entre 80 y 90% fueron resistentes a trimetoprim-sulfametoxazol, ampicilina y tetraciclina. Conclusiones: El serogrupo más frecuente fue Shigella flexneri, no se reportó Shigella dysenteriae. Existe elevado nivel de resistencia a Sulfametoxazole/trimetoprim, ampicilina y tetraciclina.

scholarly journals Glimpse on shigellosis from endemic Bangladesh: decades of observation with urban-rural differentials

2016 ◽  
Vol 4 (2) ◽  
pp. 105-109
Author(s):  
Dipesh Kumar Barua ◽  
Sumon Kumar Das ◽  
Abu Syed Golam Faruque ◽  
Mohammad Habibur Rahman Sarker ◽  
Jui Das ◽  
...  
Keyword(s):  
Family Income ◽  
Economic Status ◽  
Shigella Flexneri ◽  
Diarrhoeal Disease ◽  
Protective Role ◽  
Shigella Sonnei ◽  
Shigella Dysenteriae ◽  
Moderate Increase ◽  
Shigella Boydii ◽  
Shigella Species

The Diarrhoeal Disease Surveillence System of icddr,b noted increasing proportion of Shigella sonnei and Shigella flexneri at urban Dhaka and rural Matlab from 2000 to 2013. Shigella sonnei appeared as emerging serogroup during 2009-13 compared to 2000-04 among individuals aged ? 60 years both in Dhaka and Matlab, while Shigella boydii and Shigella flexneri showed remarkable increase with 400% and 100% respectively in Dhaka, and a moderate increase (55%) both Shigella boydii and Shigella flexneri in Matlab. However, decreased trend of all the Shigella species in both the sites except Shigella dysenteriae in Dhaka was found among under-5 children. Individuals from higher socio-economic status such as those having a monthly family income of >100 USD had protective role against shigellosis in Dhaka during 2009-13. Shigella species and host factors are responsible for changing trend as well as predictors of shigellosis.Bangladesh Crit Care J September 2016; 4 (2): 105-109

scholarly journals Gene Cloning and Characterization of VcrM, a Na+-Coupled Multidrug Efflux Pump, fromVibrio choleraeNon-O1

2003 ◽  
Vol 47 (6) ◽  
pp. 419-427 ◽  
Author(s):  
Md. Nazmul Huda ◽  
Jing Chen ◽  
Yuji Morita ◽  
Teruo Kuroda ◽  
Tohru Mizushima ◽  
...  
Keyword(s):  
Gene Cloning ◽  
Efflux Pump ◽  
Multidrug Efflux ◽  
Multidrug Efflux Pump ◽  
Gene Cloning And Characterization

Gene cloning and characterization of a trehalose synthase from Corynebacterium glutamicum ATCC13032

2010 ◽  
Vol 19 (2) ◽  
pp. 565-569 ◽  
Author(s):  
Tae-Kyun Kim ◽  
Jun-Hyuck Jang ◽  
Hong-Yeon Cho ◽  
Heung-Shick Lee ◽  
Young-Wan Kim
Keyword(s):  
Gene Cloning ◽  
Corynebacterium Glutamicum ◽  
Trehalose Synthase ◽  
Gene Cloning And Characterization

Molecular Strategies for the Detection, Identification, and Differentiation between Enteroinvasive Escherichia coli and Shigella spp.

2005 ◽  
Vol 68 (2) ◽  
pp. 239-245 ◽  
Author(s):  
CESAR I. BIN KINGOMBE ◽  
MARIA-LUCIA CERQUEIRA-CAMPOS ◽  
JEFFREY M. FARBER
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Shigella Flexneri ◽  
Shigella Dysenteriae ◽  
Shigella Boydii ◽  
Epidemiological Tool ◽  
Pcr Rflp ◽  
Shigella Spp

A strategy for the detection, identification, and differentiation of enteroinvasive Escherichia coli (EIEC) and Shigella spp. has been developed. The strategy includes (i) a multiplex PCR for the amplification of two virulence genes, i.e., iuc (222 bp) and ipaH (629 bp); (ii) amplification of the ial gene (a 1,038-bp amplicon) located within a large plasmid; and (iii) restriction fragment length polymorphism (RFLP) of the ial gene amplicon. The multiplex PCR provided three patterns. Pattern 1 (iuc−/ipaH+) was found in 10 (67%) of 15 EIEC strains tested, pattern 2 (iuc+/ipaH−) in only 2 (4.4%) of 46 non-EIEC isolates, whereas pattern 3 (iuc+/ipaH+) was observed in all Shigella spp. and also in 5 (33%) of 15 EIEC strains tested. The pattern 3 EIEC strains were all positive for the ial gene. The PCR-RFLP of the ial gene amplicon using the endonuclease AclI was used to differentiate Shigella spp. from the EIEC strains that belonged to pattern 3. The ial gene was present in 21 (38%) of 56 and 6 (40%) of 15 Shigella spp. and EIEC strains tested, respectively. The PCR-RFLP of the ial gene amplicon divided the strains in two types. Type 1 did not contain the restriction enzyme site and was found in 6 (100%) of 6 EIEC strains, 4 (80%) of 5 Shigella boydii, and 4 (100%) of 4 Shigella dysenteriae strains tested. Type 2, which gave two fragments of 286 and 752 bp, was observed in 5 (83%) of 6 Shigella flexneri strains and 6 (100%) of 6 Shigella sonnei strains. Detection, identification, and differentiation of Shigella spp. and EIEC were achieved by analyses of the PCR patterns and RFLP types. To our knowledge, this is the first study to demonstrate a simple and rapid method for detecting, identifying, and differentiating, at the molecular level, Shigella spp. and EIEC strains. This method will have tremendous utility as an epidemiological tool and in helping to develop policies, risk assessments, and national and international methods for Shigella spp.

Gene cloning and characterization of a novel α-amylase from alkaliphilicAlkalimonas amylolytica

2006 ◽  
Vol 1 (11) ◽  
pp. 1258-1265 ◽  
Author(s):  
Nan Wang ◽  
Yunhua Zhang ◽  
Quanhui Wang ◽  
Jun Liu ◽  
Honglei Wang ◽  
...  
Keyword(s):  
Gene Cloning ◽  
Gene Cloning And Characterization
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