Expression of p68 RNA Helicase Is Closely Related to the Early Stage of Adipocyte Differentiation of Mouse 3T3-L1 Cells

2001 ◽  
Vol 287 (2) ◽  
pp. 435-439 ◽  
Author(s):  
Atsushi Kitamura ◽  
Makoto Nishizuka ◽  
Kei Tominaga ◽  
Tomoko Tsuchiya ◽  
Tsutomu Nishihara ◽  
...  
Keyword(s):  
Adipocyte Differentiation ◽  
Early Stage ◽  
Rna Helicase ◽  
P68 Rna Helicase

scholarly journals p68 RNA Helicase Is an Essential Human Splicing Factor That Acts at the U1 snRNA-5′ Splice Site Duplex

2002 ◽  
Vol 22 (15) ◽  
pp. 5443-5450 ◽  
Author(s):  
Zhi-Ren Liu
Keyword(s):  
Splice Site ◽  
Early Stage ◽  
Rna Helicase ◽  
Mrna Splicing ◽  
Cross Linking ◽  
Spliceosome Assembly ◽  
U1 Snrna ◽  
U1 Snrnp ◽  
P68 Rna Helicase

ABSTRACT Modulation of the interaction between U1 snRNP and the 5′ splice site (5′ss) is a key event that governs 5′ss recognition and spliceosome assembly. Using the methylene blue-mediated cross-linking method (Z. R. Liu, A. M. Wilkie, M. J. Clemens, and C. W. Smith, RNA 2:611-621, 1996), a 65-kDa protein (p65) was shown to interact with the U1-5′ss duplex during spliceosome assembly (Z. R. Liu, B. Sargueil, and C. W. Smith, Mol. Cell. Biol. 18:6910-6920, 1998). In this report, p65 was identified as p68 RNA helicase and shown to be essential for in vitro pre-mRNA splicing. Depletion of endogenous p68 RNA helicase does not affect the loading of the U1 snRNP to the 5′ss during early stage of splicing. However, dissociation of the U1 from the 5′ss is largely inhibited. The data suggest that p68 RNA helicase functions in destabilizing the U1-5′ss interactions. Furthermore, depletion of p68 RNA helicase arrested spliceosome assembly at the prespliceosome stage, suggesting that p68 may play a role in the transition from prespliceosome to spliceosome.

scholarly journals Interaction between p68 RNA helicase and Ca2+-calmodulin promotes cell migration and metastasis

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Haizhen Wang ◽  
Xueliang Gao ◽  
Jenny J. Yang ◽  
Zhi-Ren Liu
Keyword(s):  
Cell Migration ◽  
Rna Helicase ◽  
P68 Rna Helicase

Signaling to the DEAD box—Regulation of DEAD-box p68 RNA helicase by protein phosphorylations

2005 ◽  
Vol 17 (12) ◽  
pp. 1495-1504 ◽  
Author(s):  
Liuqing Yang ◽  
Chunru Lin ◽  
Zhi-Ren Liu
Keyword(s):  
Rna Helicase ◽  
Dead Box ◽  
P68 Rna Helicase ◽  
The Dead

Functional interaction between Smad, CREB binding protein, and p68 RNA helicase

2004 ◽  
Vol 324 (1) ◽  
pp. 70-76 ◽  
Author(s):  
Dennis R. Warner ◽  
Vasker Bhattacherjee ◽  
Xiaolong Yin ◽  
Saurabh Singh ◽  
Partha Mukhopadhyay ◽  
...  
Keyword(s):  
Binding Protein ◽  
Rna Helicase ◽  
Creb Binding Protein ◽  
Functional Interaction ◽  
P68 Rna Helicase

Induction of Bach1 and ARA70 gene expression at an early stage of adipocyte differentiation of mouse 3T3-L1 cells

2002 ◽  
Vol 361 (3) ◽  
pp. 629-633 ◽  
Author(s):  
Makoto NISHIZUKA ◽  
Tomoko TSUCHIYA ◽  
Tsutomu NISHIHARA ◽  
Masayoshi IMAGAWA
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Adipocyte Differentiation ◽  
Early Stage ◽  
3T3 Cells ◽  
Subtraction Method ◽  
Proliferating Cells ◽  
Genes Encoding ◽  
Nih 3T3

Using a subtraction method, we have isolated genes that are induced early in the differentiation of mouse 3T3-L1 preadipocyte cells into adipocytes. These include the genes encoding transcription factors and signalling proteins, as well as unknown genes. Bach1, a transcription factor, and ARA70, a cofactor, were rapidly induced during differentiation. The induction of these two genes was observed only in growth-arrested 3T3-L1 cells, and not in proliferating cells. In NIH-3T3 cells, no induction was observed under either set of conditions. These results strongly indicate that Bach1 and ARA70 have valuable roles at the onset of adipocyte differentiation.

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