New Insights into the Regulation of Cathepsin K Gene Expression by Osteoprotegerin Ligand

2001 ◽  
Vol 285 (2) ◽  
pp. 335-339 ◽  
Author(s):  
Susanne Corisdeo ◽  
Michael Gyda ◽  
Mone Zaidi ◽  
Baljit S. Moonga ◽  
Bruce R. Troen
Keyword(s):  
Gene Expression ◽  
Cathepsin K ◽  
Osteoprotegerin Ligand

Concanavalin A Directly Stimulates Bone-Resorbing Activity of Osteoclasts and Their Gene Expression of Cathepsin K/OC-2

1997 ◽  
Vol 234 (3) ◽  
pp. 600-604 ◽  
Author(s):  
Shinji Kakudo ◽  
Hiroshi Mano ◽  
Miho Shiokawa ◽  
Yoshihisa Mori ◽  
Masayoshi Kumegawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Concanavalin A ◽  
Cathepsin K ◽  
Bone Resorbing Activity

scholarly journals Fibroblast-like cells change gene expression of bone remodelling markers in transwell cultures

2020 ◽  
Vol 25 (1) ◽  
Author(s):  
Eliza S. Hartmann ◽  
Sabine Schluessel ◽  
Miriam I. Köhler ◽  
Felicitas Beck ◽  
Julia I. Redeker ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Remodeling ◽  
Mrna Level ◽  
Three Dimensional ◽  
Cathepsin K ◽  
Culture Conditions ◽  
Dermal Fibroblasts ◽  
Monolayer Cultures ◽  
Bone Remodeling Markers

Abstract Introduction Periprosthetic fibroblast-like cells (PPFs) play an important role in aseptic loosening of arthroplasties. Various studies have examined PPF behavior in monolayer culture systems. However, the periprosthetic tissue is a three-dimensional (3D) mesh, which allows the cells to interact in a multidirectional way. The expression of bone remodeling markers of fibroblast-like cells in a multilayer environment changes significantly versus monolayer cultures without the addition of particles or cytokine stimulation. Gene expression of bone remodeling markers was therefore compared in fibroblast-like cells from different origins and dermal fibroblasts under transwell culture conditions versus monolayer cultures. Methods PPFs from periprosthetic tissues (n = 12), osteoarthritic (OA) synovial fibroblast-like cells (SFs) (n = 6), and dermal fibroblasts (DFs) were cultured in monolayer (density 5.5 × 103/cm2) or multilayer cultures (density 8.5 × 105/cm2) for 10 or 21 days. Cultures were examined via histology, TRAP staining, immunohistochemistry (anti-S100a4), and quantitative real-time PCR. Results Fibroblast-like cells (PPFs/SFs) and dermal fibroblasts significantly increased the expression of RANKL and significantly decreased the expression of ALP, COL1A1, and OPG in multilayer cultures. PPFs and SFs in multilayer cultures further showed a higher expression of cathepsin K, MMP-13, and TNF-α. In multilayer PPF cultures, the mRNA level of TRAP was also found to be significantly increased. Conclusion The multilayer cultures are able to induce significant expression changes in fibroblast-like cells depending on the nature of cellular origin without the addition of any further stimulus. This system might be a useful tool to get more in vivo like results regarding fibroblast-like cell cultures.

scholarly journals Localization patterns of cathepsins K and X and their predictive value in glioblastoma

2018 ◽  
Vol 52 (4) ◽  
pp. 433-442 ◽  
Author(s):  
Barbara Breznik ◽  
Clara Limback ◽  
Andrej Porcnik ◽  
Andrej Blejec ◽  
Miha Koprivnikar Krajnc ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Stem Cell ◽  
Mrna Expression ◽  
Predictive Value ◽  
Cathepsin K ◽  
Mrna Levels ◽  
Glioblastoma Stem Cells ◽  
Glioblastoma Stem Cell ◽  
Cathepsin X

AbstractBackgroundGlioblastoma is a highly aggressive central nervous system neoplasm characterized by extensive infiltration of malignant cells into brain parenchyma, thus preventing complete tumor eradication. Cysteine cathepsins B, S, L and K are involved in cancer progression and are overexpressed in glioblastoma. We report here for the first time that cathepsin X mRNA and protein are also abundantly present in malignant glioma.Materials and methodsGene expression of cathepsins K and X was analyzed using publically-available tran-scriptomic datasets and correlated with glioma grade and glioblastoma subtype. Kaplan-Maier survival analysis was performed to evaluate the predictive value of cathepsin K and X mRNA expression. Cathepsin protein expression was localized and semi-quantified in tumor tissues by immunohistochemistry.ResultsHighest gene expression of cathepsins K and X was found in glioblastoma, in particular in the mesenchymal subtype. Overall, high mRNA expression of cathepsin X, but not that of cathepsin K, correlated with poor patients’ survival. Cathepsin K and X proteins were abundantly and heterogeneously expressed in glioblastoma tissue. Immuno-labeling of cathepsins K and X was observed in areas of CD133-positive glioblastoma stem cells, localized around arterioles in their niches that also expressed SDF-1α and CD68. mRNA levels of both cathepsins K and X correlated with mRNA levels of markers of glioblastoma stem cells and their niches.ConclusionsThe presence of both cathepsins in glioblastoma stem cell niche regions indicates their possible role in regulation of glioblastoma stem cell homing in their niches. The clinical relevance of this data needs to be elaborated in further prospective studies.

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