Establishment of an Expression Cloning System for CD4+ T Cell Epitopes

2001 ◽  
Vol 284 (5) ◽  
pp. 1140-1147 ◽  
Author(s):  
Shinji Fujii ◽  
Yasushi Uemura ◽  
Leo Kei Iwai ◽  
Masayuki Ando ◽  
Satoru Senju ◽  
...  
Keyword(s):  
T Cell ◽  
Cd4 T Cell ◽  
Expression Cloning ◽  
T Cell Epitopes ◽  
Cloning System

Major contribution of codominant CD8 and CD4 T cell epitopes to the human cytomegalovirus-specific T cell repertoire

2005 ◽  
Vol 62 (1) ◽  
pp. 77-86 ◽  
Author(s):  
M. -D. Nastke† ◽  
L. Herrgen† ◽  
S. Walter ◽  
D. Wernet ◽  
H. -G. Rammensee ◽  
...  
Keyword(s):  
T Cell ◽  
Human Cytomegalovirus ◽  
Cd4 T Cell ◽  
T Cell Epitopes ◽  
T Cell Repertoire ◽  
Cell Repertoire

scholarly journals Identification of a CD4+ T cell-stimulating antigen of pathogenic bacteria by expression cloning.

1995 ◽  
Vol 182 (6) ◽  
pp. 1751-1757 ◽  
Author(s):  
S Sanderson ◽  
D J Campbell ◽  
N Shastri
Keyword(s):  
T Cells ◽  
Listeria Monocytogenes ◽  
T Cell ◽  
Mhc Class Ii ◽  
T Cell Activation ◽  
Pathogenic Bacteria ◽  
Genomic Library ◽  
Class Ii ◽  
Cd4 T Cell ◽  
Expression Cloning

Identifying the immunogenic proteins that elicit pathogen-specific T cell responses is key to rational vaccine design. While several approaches have succeeded in identifying major histocompatibility complex (MHC) class I bound peptides that stimulate CD8+ T cells, these approaches have been difficult to extend to peptides presented by MHC class II molecules that stimulate CD4+ T cells. We describe here a novel strategy for identifying CD4+ T cell-stimulating antigen genes. Using Listeria monocytogenes-specific, lacZ-inducible T cells as single-cell probes, we screened a Listeria monocytogenes genomic library as recombinant Escherichia coli that were fed to macrophages. The antigen gene was isolated from the E. coli clone that, when ingested by the macrophages, allowed generation of the appropriate peptide/MHC class II complex and T cell activation. We show that the antigenic peptide is derived from a previously unknown listeria gene product with characteristics of a membrane-bound protein.

Identification of CD4+T cell epitopes ofTaenia soliumparamyosin

2003 ◽  
Vol 25 (10) ◽  
pp. 513-516 ◽  
Author(s):  
H. S. López-Moreno ◽  
D. Correa ◽  
J. P. Laclette ◽  
V. F. Ortiz-Navarrete
Keyword(s):  
T Cell ◽  
Cd4 T Cell ◽  
T Cell Epitopes

scholarly journals Establishment of HLA-DR4 Transgenic Mice for the Identification of CD4+ T Cell Epitopes of Tumor-Associated Antigens

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e84908 ◽  
Author(s):  
Junji Yatsuda ◽  
Atsushi Irie ◽  
Kumiko Harada ◽  
Yayoi Michibata ◽  
Hirotake Tsukamoto ◽  
...  
Keyword(s):  
T Cell ◽  
Transgenic Mice ◽  
Cd4 T Cell ◽  
T Cell Epitopes ◽  
Tumor Associated Antigens

scholarly journals Predicting CD4 T-cell epitopes based on antigen cleavage, MHCII presentation, and TCR recognition

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0206654 ◽  
Author(s):  
Dina Schneidman-Duhovny ◽  
Natalia Khuri ◽  
Guang Qiang Dong ◽  
Michael B. Winter ◽  
Eric Shifrut ◽  
...  
Keyword(s):  
T Cell ◽  
Cd4 T Cell ◽  
T Cell Epitopes

scholarly journals Identification of Rotavirus VP6-Specific CD4+ T Cell Epitopes in a G1P[8] Human Rotavirus-Infected Rhesus Macaque

2008 ◽  
Vol 1 ◽  
pp. VRT.S563 ◽  
Author(s):  
Wei Zhao ◽  
Bapi Pahar ◽  
Karol Sestak
Keyword(s):  
T Cell ◽  
Rhesus Macaques ◽  
Immunoglobulin A ◽  
Cd4 T Cell ◽  
T Cell Epitopes ◽  
Primate Model ◽  
Human Rotavirus ◽  
Ifn Γ ◽  
Rotavirus Vp6

A non-human primate model was used to evaluate its potential for identification of rotavirus viral protein 6 (VP6) CD4+ T cell epitopes. Four juvenile rhesus macaques were inoculated with a mixed inoculum (G1P[8] and G9P[8]) of human rotaviruses. Infection accompanied by G1P[8] shedding was achieved in the two macaques that had no rotavirus immunoglobulin A (IgA) in plasma. To measure the interferon gamma (IFN-γ) and tumor necrosis factor (TNF) anti-viral cytokines produced by peripheral CD4+ cells that recognize VP6 epitopes, whole blood cells from one infected macaque were stimulated in vitro with VP6 peptides. Stimulation with peptide pools derived from the simian rotavirus VP6161–395 region revealed reactivity of CD4+ T cells with the VP6281–331 domain. A VP6301–315 region was identified as the epitope responsible for IFN-γ production while a broader VP6293–327 domain was linked to TNF production. These results suggest that human rotavirus-infected macaques can be used for identification of additional epitopes and domains to address specific questions related to the development of pediatric vaccines.

The CD4+ T-cell response to adenovirus is focused against conserved residues within the hexon protein

2007 ◽  
Vol 88 (9) ◽  
pp. 2417-2425 ◽  
Author(s):  
David Onion ◽  
Laura J. Crompton ◽  
Donald W. Milligan ◽  
Paul A. H. Moss ◽  
Steven P. Lee ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cell Response ◽  
Protein A ◽  
T Cell Response ◽  
Cd4 T Cell ◽  
T Cell Epitopes ◽  
Hexon Protein ◽  
Healthy Donors ◽  
Fiber Proteins

Adenovirus is a significant pathogen in immunocompromised patients and is widely utilized as a gene delivery vector, so a detailed understanding of the human immune response to adenovirus infection is critical. This study characterized the adenovirus-specific CD4+ T-cell response of healthy donors by incubation with whole virus or with individual hexon and fiber proteins. Adenovirus-specific CD4+ T cells averaged 0.26 % of the CD4+ T-cell pool and were detectable in all donors. T cells recognizing the highly conserved hexon protein accounted for 0.09 %, whereas no response was observed against the fiber protein. A panel of hexon-specific CD4+ T-cell clones was generated and shown to lyse targets infected with adenovirus from different serotypes and species. Three CD4 T-cell epitopes are described, which map to highly conserved regions of the hexon protein.

In silico accelerated identification of structurally conserved CD8+ and CD4+ T-cell epitopes in high-risk HPV types

2012 ◽  
Vol 12 (7) ◽  
pp. 1513-1518 ◽  
Author(s):  
Shishir K. Gupta ◽  
Mugdha Srivastava ◽  
Bashir A. Akhoon ◽  
Shailendra K. Gupta ◽  
Niels Grabe
Keyword(s):  
T Cell ◽  
High Risk ◽  
In Silico ◽  
Cd4 T Cell ◽  
T Cell Epitopes ◽  
Hpv Types ◽  
High Risk Hpv
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