Cloning of a Novel G Protein-Coupled Receptor, SLT, a Subtype of the Melanin-Concentrating Hormone Receptor

2001 ◽  
Vol 283 (5) ◽  
pp. 1013-1018 ◽  
Author(s):  
Masaaki Mori ◽  
Mioko Harada ◽  
Yasuko Terao ◽  
Tsukasa Sugo ◽  
Takuya Watanabe ◽  
...  
Keyword(s):  
G Protein ◽  
Hormone Receptor ◽  
G Protein Coupled Receptor ◽  
Melanin Concentrating Hormone ◽  
G Protein Coupled

The receptor for the orexigenic peptide melanin-concentrating hormone is a G-protein-coupled receptor

10.1038/12978 ◽  
1999 ◽  
Vol 1 (5) ◽  
pp. 267-271 ◽  
Author(s):  
Paola M.C. Lembo ◽  
Eric Grazzini ◽  
Jack Cao ◽  
Douglas A. Hubatsch ◽  
Manon Pelletier ◽  
...  
Keyword(s):  
G Protein ◽  
G Protein Coupled Receptor ◽  
Orexigenic Peptide ◽  
Melanin Concentrating Hormone ◽  
G Protein Coupled

scholarly journals Role of Helix 8 of the Thyrotropin-Releasing Hormone Receptor in Phosphorylation by G Protein-Coupled Receptor Kinase

2009 ◽  
Vol 77 (2) ◽  
pp. 288-297 ◽  
Author(s):  
Austin U. Gehret ◽  
Brian W. Jones ◽  
Phuong N. Tran ◽  
Laurie B. Cook ◽  
Emileigh K. Greuber ◽  
...  
Keyword(s):  
G Protein ◽  
Hormone Receptor ◽  
Thyrotropin Releasing Hormone ◽  
Receptor Kinase ◽  
G Protein Coupled Receptor ◽  
Releasing Hormone ◽  
G Protein Coupled

scholarly journals Interaction of Neurochondrin with the Melanin-concentrating Hormone Receptor 1 Interferes with G Protein-coupled Signal Transduction but Not Agonist-mediated Internalization

2006 ◽  
Vol 281 (43) ◽  
pp. 32496-32507 ◽  
Author(s):  
Felix Francke ◽  
Richard J. Ward ◽  
Laura Jenkins ◽  
Elaine Kellett ◽  
Dietmar Richter ◽  
...  
Keyword(s):  
Signal Transduction ◽  
G Protein ◽  
Hormone Receptor ◽  
Mammalian Cells ◽  
Deletion Mapping ◽  
Related Factor ◽  
C Terminus ◽  
Melanin Concentrating Hormone ◽  
G Protein Coupled

Screening of a human brain cDNA library using the C-terminal tail of the melanin-concentrating hormone receptor 1 (MCHR1) as bait in a yeast two-hybrid assay resulted in the identification of the neurite-outgrowth related factor, neurochondrin. This interaction was verified in overlay, pulldown, and co-immunoprecipitation assays. Deletion mapping confined the binding to the C terminus of neurochondrin and to the proximal C terminus of MCHR1, a region known to be involved in G protein binding and signal transduction. This region of the MCHR1 is also able to interact with the actin- and intermediate filament-binding protein, periplakin. Interactions of MCHR1 with neurochondrin and periplakin were competitive, indicating that these two proteins bind to overlapping regions of MCHR1. Although neurochondrin did not interfere with melanin-concentrating hormone-mediated internalization of the receptor, it did inhibit G protein-coupled signal transduction via both Gαi/o and Gαq/11 family G proteins as measured by each of melanin-concentrating hormone-induced G protein-activated inwardly rectifying K+ channel activity of voltage-clamped amphibian oocytes, by calcium mobilization in transfected mammalian cells, and by reduction in the capacity of melanin-concentrating hormone to promote binding of [35S]guanosine 5′-3-O-(thio)triphosphate to both Gαo1 and Gα11. Immunohistochemistry revealed co-expression of neurochondrin and MCHR1 within the rodent brain, suggesting that neurochondrin may be involved in the regulation of MCHR1 signaling and play a role in modulating melanin-concentrating hormone-mediated functions in vivo.

Melanin-concentrating hormone is the cognate ligand for the orphan G-protein-coupled receptor SLC-1

Nature ◽  
10.1038/22313 ◽  
1999 ◽  
Vol 400 (6741) ◽  
pp. 261-265 ◽  
Author(s):  
Jon Chambers ◽  
Robert S. Ames ◽  
Derk Bergsma ◽  
Alison Muir ◽  
Laura R. Fitzgerald ◽  
...  
Keyword(s):  
G Protein ◽  
G Protein Coupled Receptor ◽  
Melanin Concentrating Hormone ◽  
G Protein Coupled
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