In Vitro Selection of the RNA Aptamer against the Sialyl Lewis X and Its Inhibition of the Cell Adhesion

2001 ◽  
Vol 281 (1) ◽  
pp. 237-243 ◽  
Author(s):  
Sunjoo Jeong ◽  
Tae-Yeon Eom ◽  
Se-Jin Kim ◽  
Seong-Wook Lee ◽  
Jaehoon Yu
Keyword(s):  
Cell Adhesion ◽  
In Vitro Selection ◽  
Sialyl Lewis X ◽  
Rna Aptamer ◽  
Lewis X ◽  
Sialyl Lewis ◽  
Selection Of

scholarly journals Evaluation of Strategies Aimed at Improving Liver Progenitor Cell Rolling and Subsequent Adhesion to the Endothelium

2020 ◽  
Vol 29 ◽  
pp. 096368972091270
Author(s):  
Pierre Edouard Dollet ◽  
Mei Ju Hsu ◽  
Jérôme Ambroise ◽  
Milena Rozzi ◽  
Joachim Ravau ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Adhesion ◽  
Alternative Methods ◽  
Sialyl Lewis X ◽  
Thermosensitive Polymer ◽  
Lewis X ◽  
Promising Alternative ◽  
Sialyl Lewis ◽  
Selectin Ligand

Adult-derived human liver stem/progenitor cells (ADHLSCs) are a promising alternative to orthotopic liver transplantation in the treatment of inborn errors of metabolism. However, as is the case with many mesenchymal stromal cells, ADHLSCs have shown a low level of engraftment, which could be explained by the fact that they lack expression of selectin ligand and LFA-1 and only slightly express VLA- 4, molecules that have been shown to be involved in cell adhesion to the endothelium. In this paper, we have investigated strategies to increase their rolling and adhesion during the homing process by (1) adding a selectin ligand (Sialyl Lewis X) to their surface using biotinyl- N-hydroxy-succinimide–streptavidin bridges, and (2) protecting the adhesion proteins from trypsinization-induced damage using a thermosensitive polymer for cell culture and a nonenzymatic cell dissociation solution (CDS) for harvest. Despite increasing adhesion of ADHLSCs to E-selectin during an adhesion test in vitro performed under shear stress, the addition of Sialyl Lewis X did not increase adhesion to endothelial cells under the same conditions. Cultivating cells on a thermosensitive polymer and harvesting them with CDS increased their adhesion to endothelial cells under noninflammatory conditions, compared to the use of trypsin. However, we were not able to demonstrate any improvement in cell adhesion to the endothelium following culture on polymer and harvest with CDS, suggesting that alternative methods of improving engraftment still need to be evaluated.

scholarly journals Effect of sialyl Lewis X-glycoliposomes on the inhibition of E-selectin-mediated tumour cell adhesion in vitro

2004 ◽  
Vol 1660 (1-2) ◽  
pp. 31-40 ◽  
Author(s):  
Reinhard Zeisig ◽  
Renate Stahn ◽  
Katrin Wenzel ◽  
Diana Behrens ◽  
Iduna Fichtner
Keyword(s):  
Cell Adhesion ◽  
Tumour Cell ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
Sialyl Lewis

scholarly journals In vitro selection of RNA aptamer to hemin

2008 ◽  
Vol 52 (1) ◽  
pp. 513-514 ◽  
Author(s):  
M. Liu ◽  
T. Kagahara ◽  
H. Abe ◽  
Y. Ito
Keyword(s):  
In Vitro Selection ◽  
Rna Aptamer ◽  
Selection Of

scholarly journals Mechanical Shedding of L-selectin from the Neutrophil Surface during Rolling on Sialyl Lewis x under Flow

2006 ◽  
Vol 282 (7) ◽  
pp. 4812-4820 ◽  
Author(s):  
Dooyoung Lee ◽  
Joanne B. Schultz ◽  
Philip A. Knauf ◽  
Michael R. King
Keyword(s):  
Flow Chamber ◽  
Mechanical Force ◽  
Mitogen Activated Protein Kinase ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
Rolling Velocity ◽  
Sialyl Lewis ◽  
Mitogen Activated Protein ◽  
Neutrophil Surface

The interaction of L-selectin expressed on leukocytes with endothelial cells leads to capture and rolling and is critical for the recruitment of leukocytes into sites of inflammation. It is known that leukocyte activation by chemoattractants, the change of osmotic pressure in cell media, or cross-linking of L-selectin all result in rapid shedding of L-selectin. Here we present a novel mechanism for surface cleavage of L-selectin on neutrophils during rolling on a sialyl Lewis x-coated surface that involves mechanical force. Flow cytometry and rolling of neutrophils labeled with Qdot®-L-selectin antibodies in an in vitro flow chamber showed that the mechanical shedding of L-selectin occurs during rolling and depends on the amount of shear applied. In addition, the mechanical L-selectin shedding causes an increase in cell rolling velocity with rolling duration, suggesting a gradual loss of L-selectin and is mediated by p38 mitogen-activated protein kinase activation. Thus, these data show that mechanical force induces the cleavage of L-selectin from the neutrophil surface during rolling and therefore decreases the adhesion of cells to a ligand-presenting surface in flow.

scholarly journals Suppression of Sialyl Lewis X Expression and E-selectin-mediated Cell Adhesion in Cultured Human Lymphoid Cells by Transfection of Antisense cDNA of an α1→3 Fucosyltransferase (Fuc-T VII)

1996 ◽  
Vol 271 (49) ◽  
pp. 31556-31561 ◽  
Author(s):  
Nozomu Hiraiwa ◽  
Taeko Dohi ◽  
Naoko Kawakami-Kimura ◽  
Miki Yumen ◽  
Katsuyuki Ohmori ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Lymphoid Cells ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
Sialyl Lewis

Glycan engineering using synthetic ManNAc analogues enhances sialyl-Lewis-X expression and adhesion of leukocytes

2021 ◽  
Author(s):  
Anam Tasneem ◽  
Shubham Parashar ◽  
Tanya Jain ◽  
Simran Aittan ◽  
Jyoti Rautela ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Inflammatory Diseases ◽  
Leukocyte Adhesion ◽  
Fold Increase ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
Sialyl Lewis ◽  
Acute Myeloid Leukemia Cells ◽  
Coated Surfaces ◽  
Cell Cell

Cell surface glycans, depending on their structures and dynamic modifications, act as the first point of contact and regulate cell-cell, cell-matrix, and cell-pathogen interactions. Particularly, the sialyl-Lewis-X (sLeX, CD15s) tetrasaccharide epitope, expressed on both glycoproteins and gangliosides, participates in leukocyte extravasation via interactions with selectins expressed on endothelial cells, lymphocytes, and platelets (CD62-E/L/P). Neutrophils carrying sLeX epitopes are thought to be responsible for chronic inflammatory diseases resulting in plaque formation and atherosclerosis. Intense efforts have been devoted to the development of sLeX mimetics for inhibition of cell adhesion. On the other hand, dysregulated expression of sLeX and poor extravasation are the major underlying causes of leukocyte adhesion deficiency-II (LAD-II) disorders that result in frequent infections and poor immune response. We hypothesized that metabolic processing of peracetyl N-(cycloalkyl)acyl-D-mannosamine derivatives, through the sialic acid pathway, might result in the expression of sialoglycans with altered hydrophobicity which in-turn could modulate their binding to endogenous lectins, including selectins. Herein, we show that treatment of HL-60 (human acute myeloid leukemia) cells with peracetyl N-cyclobutanoyl-D-mannosamine (Ac4ManNCb), at 50 microM for 48 h, resulted in a robust three to four fold increase in the binding of anti-sLeX (CSLEX1) antibody and enhanced cell adhesion to E-selectin coated surfaces; while the corresponding straight-chain analogue, peracetyl N-pentanoyl-D-mannosamine (Ac4ManNPent), and peracetyl N-cyclopropanoyl-D-mannosamine (Ac4ManNCp) both resulted in 2.0-2.5fold increase compared to controls. The ability to enhance sLeX expression using small molecules has the potential to provide novel opportunities to address challenges in the treatment of immune deficiency disorders.

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