-Aspartate in a Prolactin-Secreting Clonal Strain of Rat Pituitary Tumor Cells (GH3)

2000 ◽  
Vol 279 (1) ◽  
pp. 305-306
Author(s):  
Zhiqung Long ◽  
Jen-Ai Lee ◽  
Taizo Okamoto ◽  
Noriyuki Nimura ◽  
Kazuhiro Imai ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Pituitary Tumor ◽  
Clonal Strain ◽  
Rat Pituitary ◽  
Rat Pituitary Tumor Cells

-Aspartate in a Prolactin-Secreting Clonal Strain of Rat Pituitary Tumor Cells (GH3)

2000 ◽  
Vol 276 (3) ◽  
pp. 1143-1147 ◽  
Author(s):  
Zhiqung Long ◽  
Jen-Ai Lee ◽  
Taizo Okamoto ◽  
Noriyuki Nimura ◽  
Kazuhiro Imai ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Pituitary Tumor ◽  
Clonal Strain ◽  
Rat Pituitary ◽  
Rat Pituitary Tumor Cells

scholarly journals Prolactin storage in a clonal strain of rat pituitary tumor cells is cell-cycle dependent.

1982 ◽  
Vol 93 (2) ◽  
pp. 459-462 ◽  
Author(s):  
D R Kiino ◽  
D E Burger ◽  
P S Dannies
Keyword(s):  
Cell Cycle ◽  
Cell Growth ◽  
Tumor Cells ◽  
Pituitary Tumor ◽  
Fluorescein Isothiocyanate ◽  
Cell Cycle Distribution ◽  
Clonal Strain ◽  
Rat Pituitary ◽  
Rat Pituitary Tumor Cells ◽  
Cycle Dependent

GH4C1 cells (CH cells) are a clonal strain of rat pituitary tumor cells which secrete prolactin. We measured intracellular prolactin at different stages of the cell cycle using flow microfluorometry. Prolactin was stained by an indirect immunocytochemical technique using fluorescein isothiocyanate (FITC)-conjugated antiserum, and DNA was stained simultaneously with propidium iodide. We found that prolactin storage in GH cells was cell-cycle dependent; prolactin storage increased as cells passed from G1 to S to G2 + M. We have shown previously that insulin and 17 beta-estradiol act synergistically to increase intracellular prolactin three- to sevenfold and slow the rate of cell growth to approximately 70% of control cells. In this study we observed that insulin and estradiol increased prolactin storage at each stage of the cell cycle but did not affect the cell-cycle distribution of the population even though cell growth was slowed. We conclude that insulin and estradiol did not increase prolactin storage by affecting the cell-cycle distribution of the population.

scholarly journals Regulated expression of the prolactin gene in rat pituitary tumor cells.

1980 ◽  
Vol 87 (1) ◽  
pp. 6-13 ◽  
Author(s):  
B A Brennessel ◽  
D K Biswas
Keyword(s):  
Tumor Cells ◽  
Pituitary Tumor ◽  
Rna Sequences ◽  
Gh Cells ◽  
Prolactin Gene ◽  
Rat Pituitary ◽  
Cell Strains ◽  
Rat Pituitary Tumor Cells ◽  
Prl Gene ◽  
Specific Mrna

Prolactin (PRL) gene expression in three strains of GH cells (rat pituitary tumor cells) has been quantitated by measurement of: (a) intracellular and extracellular PRL, (b) cytoplasmic translatable PRL-specific mRNA (mRNAPRL), and (c) molecular hybridization of cytoplasmic poly(A) RNA to cDNAPRL (DNA complementary to mRNAPRL). Three GH cell lines utilized in this investigation were a PRL-producing (PRL+) strain, GH4C1, a PRL nonproducing 5-bromo-deoxyuridine resistnat (PRL- BrdUrdr) strain, F1BGH12C1, and a new strain, 928-9b, derived by fusion of PRL+ cells with a nuclear monolayer of the PRL-, BrdUrdr GH cell strain. PRL production is a characteristic of 928-9b cells, but the level of PRL production (2-4 micrograms/mg protein/24 h) is much lower than that of the PRL+ strain, GH4C1 (15-25 micrograms/mg protein/24 h). Levels of cytoplasmic translatable mRNAPRL and cytoplasmic PRL-RNA sequences quantitated with a cDNAPRL probe were also much lower in 928-9b as compared to the PRL+ parent. PRL-RNA sequences could not be detected in the PRL- strain. Thyrotopin-releasing hormone (TRH) stimulates PRL synthesis about threefold and inhibit a growth hormone (GH) synthesis 72% in the PRL+ strain. TRH has no effect on the synthesis of either PRL or GH in the 928-9b strain, although TRH receptors could be detected in these cells. Stimulation of PRL synthesis in the PRL+ strain by TRH could be correlated with increases in levels of cytoplasmic translatable mRNAPRL and increases in cytoplasmic PRL-RNA sequences. These results demonstrate that the graded expression of the PRL gene at the basal level, and in response to TRH, is caused by the regulated production of specific mRNA, i.e., mRNAPRL in these three GH cell strains.

Secretogranin I (chromogranin B) mRNA accumulation is hormonally regulated in GH3B6 rat pituitary tumor cells

1991 ◽  
Vol 80 (1-3) ◽  
pp. 41-51 ◽  
Author(s):  
J.N. Laverrière ◽  
J.L. Richard ◽  
A. Morin ◽  
N. Buisson ◽  
A. Tixier-Vidal ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Pituitary Tumor ◽  
Chromogranin B ◽  
Mrna Accumulation ◽  
Rat Pituitary ◽  
Rat Pituitary Tumor Cells ◽  
Secretogranin I
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