Attenuation of Expression of γ-Glutamylcysteine Synthetase by Ribozyme Transfection Enhance Insulin Secretion by Pancreatic β Cell Line, MIN6

2000 ◽  
Vol 278 (1) ◽  
pp. 236-240 ◽  
Author(s):  
Hideaki Kondo ◽  
Satoko Mori ◽  
Hirofumi Takino ◽  
Hiroshi Kijima ◽  
Hironori Yamasaki ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Cell Line ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
Glutamylcysteine Synthetase

scholarly journals Investigation of Ultrasound-Mediated Intracellular Ca2+ Oscillations in HIT-T15 Pancreatic β-Cell Line

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1129
Author(s):  
Chi Woo Yoon ◽  
Nan Sook Lee ◽  
Kweon Mo Koo ◽  
Sunho Moon ◽  
Kyosuk Goo ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Cell Line ◽  
Purinergic Signaling ◽  
Underlying Mechanism ◽  
Resting Cells ◽  
Pancreatic Β Cell ◽  
Pulsed Ultrasound ◽  
Β Cell ◽  
Secretion Pathways ◽  
Glucose Stimulated Insulin Secretion

In glucose-stimulated insulin secretion (GSIS) of pancreatic β-cells, the rise of free cytosolic Ca2+ concentration through voltage-gated calcium channels (VGCCs) triggers the exocytosis of insulin-containing granules. Recently, mechanically induced insulin secretion pathways were also reported, which utilize free cytosolic Ca2+ ions as a direct regulator of exocytosis. In this study, we aimed to investigate intracellular Ca2+ responses on the HIT-T15 pancreatic β-cell line upon low-intensity pulsed ultrasound (LIPUS) stimulation and found that ultrasound induces two distinct types of intracellular Ca2+ oscillation, fast-irregular and slow-periodic, from otherwise resting cells. Both Ca2+ patterns depend on the purinergic signaling activated by the rise of extracellular ATP or ADP concentration upon ultrasound stimulation, which facilitates the release through mechanosensitive hemichannels on the plasma membrane. Further study demonstrated that two subtypes of purinergic receptors, P2X and P2Y, are working in a competitive manner depending on the level of glucose in the cell media. The findings can serve as an essential groundwork providing an underlying mechanism for the development of a new therapeutic approach for diabetic conditions with further validation.

scholarly journals The biphasic effect of wortmannin on insulin secretion from pancreatic β cell line MIN6.

1994 ◽  
Vol 64 ◽  
pp. 112
Author(s):  
Takashi Sakurai ◽  
Shinji Hagiwara ◽  
Fumi Tashiro ◽  
Yoshiaki Hashimoto ◽  
Hiroshi Yano ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Cell Line ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
Biphasic Effect

scholarly journals A genetically engineered human pancreatic β cell line exhibiting glucose-inducible insulin secretion

2011 ◽  
Vol 121 (9) ◽  
pp. 3589-3597 ◽  
Author(s):  
Philippe Ravassard ◽  
Yasmine Hazhouz ◽  
Séverine Pechberty ◽  
Emilie Bricout-Neveu ◽  
Mathieu Armanet ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Cell Line ◽  
Genetically Engineered ◽  
Pancreatic Β Cell ◽  
Β Cell

scholarly journals Arachidonic acid actions on functional integrity and attenuation of the negative effects of palmitic acid in a clonal pancreatic β-cell line

2010 ◽  
Vol 120 (5) ◽  
pp. 195-206 ◽  
Author(s):  
Deirdre C. Keane ◽  
Hilton K. Takahashi ◽  
Shalinee Dhayal ◽  
Noel G. Morgan ◽  
Rui Curi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Insulin Secretion ◽  
Fatty Acid ◽  
Palmitic Acid ◽  
Cell Line ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
Lipoxygenase Inhibitors ◽  
Cox 1

Chronic exposure of pancreatic β-cells to saturated non-esterified fatty acids can lead to inhibition of insulin secretion and apoptosis. Several previous studies have demonstrated that saturated fatty acids such as PA (palmitic acid) are detrimental to β-cell function compared with unsaturated fatty acids. In the present study, we describe the effect of the polyunsaturated AA (arachidonic acid) on the function of the clonal pancreatic β-cell line BRIN-BD11 and demonstrate AA-dependent attenuation of PA effects. When added to β-cell incubations at 100 μM, AA can stimulate cell proliferation and chronic (24 h) basal insulin secretion. Microarray analysis and/or real-time PCR indicated significant AA-dependent up-regulation of genes involved in proliferation and fatty acid metabolism [e.g. Angptl (angiopoietin-like protein 4), Ech1 (peroxisomal Δ3,5,Δ2,4-dienoyl-CoA isomerase), Cox-1 (cyclo-oxygenase-1) and Cox-2, P<0.05]. Experiments using specific COX and LOX (lipoxygenase) inhibitors demonstrated the importance of COX-1 activity for acute (20 min) stimulation of insulin secretion, suggesting that AA metabolites may be responsible for the insulinotropic effects. Moreover, concomitant incubation of AA with PA dose-dependently attenuated the detrimental effects of the saturated fatty acid, so reducing apoptosis and decreasing parameters of oxidative stress [ROS (reactive oxygen species) and NO levels] while improving the GSH/GSSG ratio. AA decreased the protein expression of iNOS (inducible NO synthase), the p65 subunit of NF-κB (nuclear factor κB) and the p47 subunit of NADPH oxidase in PA-treated cells. These findings indicate that AA has an important regulatory and protective β-cell action, which may be beneficial to function and survival in the ‘lipotoxic’ environment commonly associated with Type 2 diabetes mellitus.

scholarly journals Amino acid taste receptor regulates insulin secretion in pancreatic β-cell line MIN6 cells

2011 ◽  
Vol 16 (5) ◽  
pp. 608-616 ◽  
Author(s):  
Manami Oya ◽  
Hideyuki Suzuki ◽  
Yuichiro Watanabe ◽  
Moritoshi Sato ◽  
Takashi Tsuboi
Keyword(s):  
Insulin Secretion ◽  
Amino Acid ◽  
Cell Line ◽  
Taste Receptor ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
Min6 Cells
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