Hepatic Copper Accumulation Induces DNA Strand Breaks in the Liver Cells of Long-Evans Cinnamon Strain Rats

2000 ◽  
Vol 276 (1) ◽  
pp. 174-178 ◽  
Author(s):  
Masanobu Hayashi ◽  
Tomoko Kuge ◽  
Daiji Endoh ◽  
Kenji Nakayama ◽  
Jiro Arikawa ◽  
...  
Keyword(s):  
Dna Strand Breaks ◽  
Liver Cells ◽  
Copper Accumulation ◽  
Strand Breaks ◽  
Hepatic Copper ◽  
Long Evans ◽  
Dna Strand

scholarly journals Hepatic Iron Accumulation Is Not Directly Associated With Induction of DNA Strand Breaks in the Liver Cells of Long-Evans Cinnamon (LEC) Rats.

2002 ◽  
Vol 51 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Masanobu HAYASHI ◽  
Tomoko KUGE ◽  
Daiji ENDOH ◽  
Kenji NAKAYAMA ◽  
Jiro ARIKAWA ◽  
...  
Keyword(s):  
Dna Strand Breaks ◽  
Liver Cells ◽  
Iron Accumulation ◽  
Hepatic Iron ◽  
Strand Breaks ◽  
Lec Rats ◽  
Long Evans ◽  
Dna Strand

scholarly journals Accumulation of Copper Induces DNA Strand Breaks in Brain Cells of Long-Evans Cinnamon (LEC) Rats, An Animal Model for Human Wilson Disease

2006 ◽  
Vol 55 (5) ◽  
pp. 419-426 ◽  
Author(s):  
Masanobu HAYASHI ◽  
Sayaka FUSE ◽  
Daiji ENDOH ◽  
Noboru HORIGUCHI ◽  
Kenji NAKAYAMA ◽  
...  
Keyword(s):  
Animal Model ◽  
Wilson Disease ◽  
Dna Strand Breaks ◽  
Brain Cells ◽  
Strand Breaks ◽  
Lec Rats ◽  
Long Evans ◽  
Dna Strand

scholarly journals Inhibitory Effects of Trientine, a Copper-Chelating Agent, on Induction of DNA Strand Breaks in Kidney Cells of Long-Evans Cinnamon (LEC) Rats

2005 ◽  
Vol 54 (5) ◽  
pp. 403-412 ◽  
Author(s):  
Masanobu HAYASHI ◽  
Kazuhiro MIYANE ◽  
Misato SENOU ◽  
Daiji ENDOH ◽  
Hidetoshi HIGUCHI ◽  
...  
Keyword(s):  
Chelating Agent ◽  
Dna Strand Breaks ◽  
Kidney Cells ◽  
Inhibitory Effects ◽  
Strand Breaks ◽  
Lec Rats ◽  
Long Evans ◽  
Dna Strand

scholarly journals Repair of DNA Strand Breaks by the Overlapping Functions of Lesion-Specific and Non-Lesion-Specific DNA 3′ Phosphatases

2001 ◽  
Vol 21 (21) ◽  
pp. 7191-7198 ◽  
Author(s):  
John R. Vance ◽  
Thomas E. Wilson
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Synthetic Lethality ◽  
Dna Strand Breaks ◽  
Triple Mutant ◽  
Phosphatase Domain ◽  
Strand Breaks ◽  
Alternative Pathways ◽  
Processing Enzymes ◽  
Dna Strand

ABSTRACT In Saccharomyces cerevisiae, the apurinic/apyrimidinic (AP) endonucleases Apn1 and Apn2 act as alternative pathways for the removal of various 3′-terminal blocking lesions from DNA strand breaks and in the repair of abasic sites, which both result from oxidative DNA damage. Here we demonstrate that Tpp1, a homologue of the 3′ phosphatase domain of polynucleotide kinase, is a third member of this group of redundant 3′ processing enzymes. Unlike Apn1 and Apn2, Tpp1 is specific for the removal of 3′ phosphates at strand breaks and does not possess more general 3′ phosphodiesterase, exonuclease, or AP endonuclease activities. Deletion ofTPP1 in an apn1 apn2 mutant background dramatically increased the sensitivity of the double mutant to DNA damage caused by H2O2 and bleomycin but not to damage caused by methyl methanesulfonate. The triple mutant was also deficient in the repair of 3′ phosphate lesions left by Tdp1-mediated cleavage of camptothecin-stabilized Top1-DNA covalent complexes. Finally, the tpp1 apn1 apn2 triple mutation displayed synthetic lethality in combination with rad52, possibly implicating postreplication repair in the removal of unrepaired 3′-terminal lesions resulting from endogenous damage. Taken together, these results demonstrate a clear role for the lesion-specific enzyme, Tpp1, in the repair of a subset of DNA strand breaks.

Sign in / Sign up

Export Citation Format

Share Document