Expression of Melanin-Concentrating Hormone Receptors in Insulin-Producing Cells: MCH Stimulates Insulin Release in RINm5F and CRI-G1 Cell-Lines

2000 ◽  
Vol 275 (2) ◽  
pp. 709-712 ◽  
Author(s):  
M. Tadayyon ◽  
H.J. Welters ◽  
A.C. Haynes ◽  
J.E. Cluderay ◽  
G. Hervieu
Keyword(s):  
Insulin Release ◽  
Cell Lines ◽  
Hormone Receptors ◽  
Insulin Producing Cells ◽  
Melanin Concentrating Hormone

Expression and characterization of melanin-concentrating hormone receptors on mammalian cell lines

Peptides ◽  
2004 ◽  
Vol 25 (10) ◽  
pp. 1585-1595 ◽  
Author(s):  
Alex N. Eberle ◽  
Gabriele Mild ◽  
Sophie Schlumberger ◽  
Roma Drozdz ◽  
Edith Hintermann ◽  
...  
Keyword(s):  
Cell Lines ◽  
Mammalian Cell ◽  
Hormone Receptors ◽  
Mammalian Cell Lines ◽  
Melanin Concentrating Hormone

Expression, localization and functional role of small GTPases of the Rab3 family in insulin-secreting cells

1996 ◽  
Vol 109 (9) ◽  
pp. 2265-2273 ◽  
Author(s):  
R. Regazzi ◽  
M. Ravazzola ◽  
M. Iezzi ◽  
J. Lang ◽  
A. Zahraoui ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Beta Cells ◽  
Insulin Release ◽  
Cell Lines ◽  
Secretory Granules ◽  
Small Gtpases ◽  
Wild Type ◽  
Gtp Hydrolysis ◽  
Human Pancreatic Islets ◽  
Insulin Secreting Cells

We examined the presence of small molecular mass GTP-binding proteins of the Rab3 family in different insulin-secreting cells. Rab3B and Rab3C were identified by western blotting in rat and in human pancreatic islets, in two rat insulin-secreting cell lines, RINm5F and INS-1, as well as in the hamster cell line HIT-T15. In contrast, Rab3A was detected in rat pancreatic islets as well as in the two insulin-secreting rat cell lines but not in human pancreatic islets and was only barely discernible in HIT-T15 cells. These findings were confirmed by two-dimensional gel electrophoresis followed by GTP-overlay of homogenates of pancreatic islets and of the purified protein. Northern blotting analysis revealed that Rab3D is expressed in the same insulin-secreting cells as Rab3A. Separation of the cells of the rat islets by fluorescence-activated cell sorting demonstrated that Rab3A was exclusively expressed in beta-cells. Rab3A was found to be associated with insulin-containing secretory granules both by immunofluorescence, immunoelectron microscopy and after sucrose density gradient. Overexpression in HIT-T15 cells of a Rab3A mutant deficient in GTP hydrolysis inhibited insulin secretion stimulated by a mixture of nutrients and bombesin. Insulin release triggered by these secretagogues was also slightly decreased by the overexpression of wild-type Rab3A but not by the overexpression of wild-type Rab5A and of a Rab5A mutant deficient in GTP hydrolysis. Finally, we studied the expression in insulin-secreting cells of rabphilin-3A, a putative effector protein that associates with the GTP-bound form of Rab3A. This Rab3A effector was not detectable in any of the cells investigated in the present study. Taken together these results indicate an involvement of Rab3A in the control of insulin release in rat and hamster. In human beta-cells, a different Rab3 isoform but with homologous function may replace Rab3A.

Insulin release from a cloned precursor beta cell line

1987 ◽  
Vol 113 (1) ◽  
pp. 3-NP ◽  
Author(s):  
K. W. Ng ◽  
P. R. Gummer ◽  
B. L. Grills ◽  
V. P. Michelangeli ◽  
M. E. Dunlop
Keyword(s):  
B Cells ◽  
B Cell ◽  
Cell Line ◽  
Insulin Release ◽  
Cell Lines ◽  
Neonatal Rat ◽  
Insulin Content ◽  
Morphological Evidence ◽  
Bicarbonate Buffer ◽  
Beta Cell Line

ABSTRACT This paper describes the establishment in long-term tissue culture of a functional, clonal beta (B) cell line UMR 407/3 derived from neonatal rat pancreas. Immunofluorescence demonstrated specific and uniform staining for insulin. Transmission electron microscopy showed the presence of microvilli and cytoplasmic granules. The doubling time in culture was approximately 60 h in 2% (v/v) fetal calf serum with inhibition of growth at confluence. Biochemical studies demonstrated the incorporation of [3H]leucine into proinsulin and insulin, with insulin comprising 43·6% of the total radioactivity incorporated into immune complexes. When incubated at 37 °C for 30 min with Krebs–Ringer bicarbonate buffer (pH 7·4), the amount of insulin released on stimulation by 16·7 mmol glucose/l, 20 mmol dl-glyceraldehyde/l or 20 mmol α-ketoisocaproate/l was significantly higher compared with 5·6 mmol glucose/l. The mean insulin content was equivalent to 99±0·4 fmol (s.e.m.)/5 × 105 cells. Regulated insulin release was maintained through at least 15 passages in culture. The cells showed morphological evidence of senescence after passage 26 and this was associated with significant reduction in stimulated insulin release as well as insulin content. The ability of the cells of this clonal line to grow in soft agar suggests that it is a precursor cell line. The clonal B cell lines isolated so far may thus represent variably committed rather than fully differentiated B cells in culture. These clonal non-neoplastic cell lines will be useful models with which to study the regulation of maturation/differentiation of B cells and insulin gene expression. Finally, the method described for the isolation of clonal B cell lines can be used to establish, in culture, precursor clonal lines from other normal tissues. J. Endocr. (1987) 113, 3–10

EXPRESSION OF RECEPTORS FOR MELANIN-CONCENTRATING HORMONE (MCH) IN DIFFERENT TISSUES AND CELL LINES

2002 ◽  
Vol 22 (1-4) ◽  
pp. 509-531 ◽  
Author(s):  
Sophie E. Schlumberger ◽  
Christiane Talke-Messerer ◽  
Urs Zumsteg ◽  
Alex N. Eberle
Keyword(s):  
Cell Lines ◽  
Melanin Concentrating Hormone ◽  
Different Tissues

scholarly journals Steroid-hormone receptors in cell lines and tumor biopsies of human lung cancer

1996 ◽  
Vol 67 (3) ◽  
pp. 357-364 ◽  
Author(s):  
Ulrich Kaiser ◽  
Jürgen Hofmann ◽  
Margret Schilli ◽  
Bärbel Wegmann ◽  
Uwe Klotz ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Lines ◽  
Hormone Receptors ◽  
Human Lung ◽  
Steroid Hormone ◽  
Steroid Hormone Receptors ◽  
Human Lung Cancer ◽  
Tumor Biopsies
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