Regulation of Connexin 43 Gene Expression by Cyclical Mechanical Stretch in Neonatal Rat Cardiomyocytes

2000 ◽  
Vol 273 (3) ◽  
pp. 1180
Author(s):  
Tzong-Luen Wang ◽  
Yung-Zu Tseng ◽  
Hang Chang
Keyword(s):  
Gene Expression ◽  
Connexin 43 ◽  
Mechanical Stretch ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes

Rapid Effects of Stretched Myocardial and Vascular Cells on Gene Expression of Neonatal Rat Cardiomyocytes with Emphasis on Autocrine and Paracrine Mechanisms

2000 ◽  
Vol 381 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Annemieke J. van Wamel ◽  
Cindy Ruwhof ◽  
Lizet J. van der Valk-Kokshoorn ◽  
Peter I. Schrier ◽  
Arnoud van der Laarse
Keyword(s):  
Gene Expression ◽  
Neonatal Rat ◽  
Vascular Cells ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes

Inhibitory effect of trilinolein on endothelin-1-induced c-fos gene expression in cultured neonatal rat cardiomyocytes

2005 ◽  
Vol 372 (2) ◽  
pp. 160-167 ◽  
Author(s):  
Hung-Yu Yang ◽  
Ju-Chi Liu ◽  
Yen-Ling Chen ◽  
Cheng-Hsien Chen ◽  
Heng Lin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inhibitory Effect ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Endothelin 1 ◽  
Neonatal Rat Cardiomyocytes

Modulation of gene expression in neonatal rat cardiomyocytes by surface modification of polylactide-co-glycolide substrates

2005 ◽  
Vol 74A (3) ◽  
pp. 419-429 ◽  
Author(s):  
David A. Brown ◽  
Ramin E. Beygui ◽  
William R. MacLellan ◽  
Hillel Laks ◽  
James C. Y. Dunn ◽  
...  
Keyword(s):  
Gene Expression ◽  
Surface Modification ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Modulation Of Gene Expression

Differential effects of overexpression of two forms of ephrin-A5 on neonatal rat cardiomyocytes

2001 ◽  
Vol 281 (6) ◽  
pp. H2738-H2746 ◽  
Author(s):  
Yun You Li ◽  
Zhibao Mi ◽  
Yiqin Feng ◽  
Charles F. McTiernan ◽  
Renping Zhou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tyrosine Kinases ◽  
Neonatal Rat ◽  
Cdna Clones ◽  
Eph Receptors ◽  
Expression Array ◽  
Rat Cardiomyocytes ◽  
Cdna Expression Array ◽  
Neonatal Rat Cardiomyocytes ◽  
Multiple Transcripts

Eph receptors constitute the largest family of receptor tyrosine kinases. Multiple transcripts of ephrin-A5, the cognate ligand of the EphA3 receptor, were found in neonatal rat cardiomyocytes. Two cDNA clones encoding the full-length ephrin-A5 (ephrin-A5α) and a 27-amino acid deletion form (ephrin-A5β) were isolated. To examine the role of ephrin-A5 in cardiomyocytes, the cDNAs were inserted into adenoviral vectors, termed Ad.ephrin-A5α and Ad.ephrin-A5β, respectively, and overexpressed in cardiomyocytes. The effect of ephrin-A5 on cardiomyocyte gene expression was investigated using a cDNA expression array and Western blot analysis. The results showed that both ephrin-A5α and ephrin-A5β downregulated cyclin D2, cyclin-dependent kinase-4 proteins, and their cognate receptor EphA3, which were associated with reduced bromodeoxyuridine incorporation in cardiomyocytes. Whereas ephrin-A5α and ephrin-A5β also induced differential gene expression, only ephrin-A5β significantly upregulated the transcription of brain natriuretic peptide and downregulated ras-related protein RAB2, protein kinase C inhibitor protein-1, clusterin, and insulin-like growth factor-binding protein. The results suggest that the two forms of ephrin-A5 share similar function while differ in regulating different sets of genes in cardiomyocytes.

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