Ca2+-Modulated Phosphorylation of a Low-Molecular-Mass Polypeptide in Rat Liver Mitochondria: Evidence That It Is Identical with Subunit c of F0F1-ATPase

2000 ◽  
Vol 270 (3) ◽  
pp. 741-744 ◽  
Author(s):  
Tamara S Azarashvily ◽  
Jaana Tyynelä ◽  
Marc Baumann ◽  
Yuri V Evtodienko ◽  
Nils-Erik L Saris
Keyword(s):  
Molecular Mass ◽  
Rat Liver ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Subunit C

scholarly journals Molecular cloning and characterization of a mitochondrial peroxisome proliferator-induced acyl-CoA thioesterase from rat liver

1998 ◽  
Vol 329 (3) ◽  
pp. 601-608 ◽  
Author(s):  
L. Thomas SVENSSON ◽  
T. Susanna ENGBERG ◽  
Toshifumi AOYAMA ◽  
Nobuteru USUDA ◽  
E. H. Stefan ALEXSON ◽  
...  
Keyword(s):  
Molecular Mass ◽  
Rat Liver ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Liver Mitochondria ◽  
Leader Peptide ◽  
Rat Liver Mitochondria ◽  
Peroxisome Proliferator ◽  
Calculated Molecular Mass

We have previously reported the purification and characterization of the peroxisome proliferator-induced very-long-chain acyl-CoA thioesterase (MTE-I) from rat liver mitochondria [L. T. Svensson, S. E. H. Alexson and J. K. Hiltunen (1995) J. Biol. Chem. 270, 12177-12183]. Here we describe the cloning of the corresponding cDNA. One full-length clone was isolated that contained an open reading frame of 1359 bp encoding a polypeptide with a calculated molecular mass of 49707 Da. The deduced amino acid sequence contains a putative mitochondrial leader peptide of 42 residues. Expression of the cDNA in Chinese hamster ovary cells, followed by immunofluorescence, immunoelectron microscopy and Western blot analyses, showed that the product was targeted to mitochondria and processed to a mature protein of 45 kDa, which is similar to the molecular mass of the protein isolated from rat liver mitochondria. The recombinant enzyme showed the same acyl-CoA chain-length specificity as the isolated rat liver enzyme. Sequence analysis showed no similarity to known esterases, but a high degree (approx. 40%) of identity with bile acid-CoA:amino acid N-acyltransferase cloned from human and rat liver. A putative active-site serine motif (Gly-Xaa-Ser-Xaa-Gly) of several carboxylesterases and lipases was identified. Western and Northern blot analyses showed that MTE-I is constitutively expressed in heart and is strongly induced in liver by feeding rats with di(2-ethylhexyl)phthalate, a peroxisome proliferator, suggesting a role for the enzyme in lipid metabolism.

scholarly journals Purification of aldehyde dehydrogenase from rat liver mitochondria by α-cyanocinnamate affinity chromatography

1989 ◽  
Vol 259 (1) ◽  
pp. 105-110 ◽  
Author(s):  
R C Poole ◽  
A P Halestrap
Keyword(s):  
Molecular Mass ◽  
Rat Liver ◽  
Aldehyde Dehydrogenase ◽  
Minor Component ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Affinity Column ◽  
A Subunit ◽  
A Minor ◽  
Related Compounds

1. alpha-Cyano-4-hydroxycinnamate was coupled to Sepharose CL-4B activated with 1,2:3,4-bisepoxybutane. 2. The low-Km rat liver mitochondrial aldehyde dehydrogenase was specifically bound to this affinity medium, and could subsequently be eluted with alpha-cyano-4-hydroxycinnamate. 3. The enzyme purified in this manner had a subunit molecular mass of 55 kDa and a pI of approx. 6.5. A minor component of approx. 57 kDa was also present and had a significantly higher pI value; this may be the precursor for aldehyde dehydrogenase. 4. alpha-Cyanocinnamate and some related compounds were found to be uncompetitive inhibitors of the enzyme. 5. No cytosolic aldehyde dehydrogenase was bound to the affinity column, but a protein from a rat liver post-mitochondrial supernatant with a molecular mass of approx. 25 kDa was bound, and could be eluted subsequently with alpha-cyano-4-hydroxycinnamate.

Effects of 5-5'-Diphenyl-2-thiohydantoin (DPTH) and Thyroxine on the Ultrastructure and Chemical Composition of Rat Liver

1971 ◽  
Vol 29 ◽  
pp. 570-571
Author(s):  
E. A. Elfont ◽  
R. B. Tobin ◽  
D. G. Colton ◽  
M. A. Mehlman
Keyword(s):  
Chemical Composition ◽  
Rat Liver ◽  
Future Study ◽  
Mode Of Action ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Effective Inhibitor ◽  
Biochemical Effects ◽  
Glycerophosphate Dehydrogenase ◽  
Stimulation Of

Summary5,-5'-diphenyl-2-thiohydantoin (DPTH) is an effective inhibitor of thyroxine (T4) stimulation of α-glycerophosphate dehydrogenase in rat liver mitochondria. Because this finding indicated a possible tool for future study of the mode of action of thyroxine, the ultrastructural and biochemical effects of DPTH and/or thyroxine on rat liver mere investigated.Rats were fed either standard or DPTH (0.06%) diet for 30 days before T4 (250 ug/kg/day) was injected. Injection of T4 occurred daily for 10 days prior to sacrifice. After removal of the liver and kidneys, part of the tissue was frozen at -50°C for later biocheailcal analyses, while the rest was prefixed in buffered 3.5X glutaraldehyde (390 mOs) and post-fixed in buffered 1Z OsO4 (376 mOs). Tissues were embedded in Araldlte 502 and the sections examined in a Zeiss EM 9S.Hepatocytes from hyperthyroid rats (Fig. 2) demonstrated enlarged and more numerous mitochondria than those of controls (Fig. 1). Glycogen was almost totally absent from the cytoplasm of the T4-treated rats.

Energy and Antioxidant Status of Rat Liver Mitochondria during Hypoxia- Reoxygenation of Different Duration

2016 ◽  
Vol 7 (4) ◽  
pp. 349-361
Author(s):  
Olga A. Gonchar ◽  
Valentina I. Nosar ◽  
Larisa. V. Bratus ◽  
I. N. Tymchenko ◽  
N. N. Steshenko ◽  
...  
Keyword(s):  
Rat Liver ◽  
Antioxidant Status ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Hypoxia Reoxygenation
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