Role of Hepatocyte Nuclear Factor 1α and 1β in the Transcriptional Regulation of Human Dipeptidyl Peptidase IV during Differentiation of Caco-2 Cells

2000 ◽  
Vol 270 (1) ◽  
pp. 235-239 ◽  
Author(s):  
Roger H. Erickson ◽  
Roy S. Lai ◽  
Young S. Kim
Keyword(s):  
Transcriptional Regulation ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Hepatocyte Nuclear Factor 1Α

scholarly journals Regulation of the gene for human dipeptidyl peptidase IV by hepatocyte nuclear factor 1α

1999 ◽  
Vol 338 (1) ◽  
pp. 91 ◽  
Author(s):  
Roger H. ERICKSON ◽  
James R. GUM ◽  
Craig D. LOTTERMAN ◽  
James W. HICKS ◽  
Roy S. LAI ◽  
...  
Keyword(s):  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Hepatocyte Nuclear Factor 1Α

scholarly journals Regulation of the gene for human dipeptidyl peptidase IV by hepatocyte nuclear factor 1α

1999 ◽  
Vol 338 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Roger H. ERICKSON ◽  
James R. GUM ◽  
Craig D. LOTTERMAN ◽  
James W. HICKS ◽  
Roy S. LAI ◽  
...  
Keyword(s):  
Transcriptional Activation ◽  
Fold Increase ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
3T3 Cells ◽  
Epithelial Cell Lines ◽  
Hepatocyte Nuclear Factor 1Α ◽  
Nuclear Factor 1

Hepatocyte nuclear factor 1 was identified as the transcription factor binding to a 20 bp (-150 to -131) region of the gene for human dipeptidyl peptidase IV, which has been shown to be important for the expression of dipeptidyl peptidase IV in the human intestinal and hepatic epithelial cell lines Caco-2 and HepG2. Functional analysis of the hepatocyte nuclear factor 1 site was performed with two minimal dipeptidyl peptidase IV promoter constructs (-250 to -41, and -150 to -41) with and without a 3 bp mutation in the hepatocyte nuclear factor 1 sequence, and used in transient transfection experiments with Caco-2 cells. The results show that the mutated constructs were able to drive transcription at only 5–10% of the activity of the non-mutated controls. Co-transfection of 3T3 cells with hepatocyte nuclear factor 1 (α or β) and dipeptidyl peptidase IV promoter constructs (-250 to -41 or -150 to -41) resulted in a 2.5–6-fold increase in transcription over controls with hepatocyte nuclear factor 1α but not with hepatocyte nuclear factor 1β. The results of this study show that hepatocyte nuclear factor 1 binds to the -150 to -131 region of the human dipeptidyl peptidase IV promoter and is necessary for transcriptional activation of the gene for dipeptidyl peptidase IV.

scholarly journals The Role of Hepatocyte Nuclear Factor-3α (Forkhead Box A1) and Androgen Receptor in Transcriptional Regulation of Prostatic Genes

2003 ◽  
Vol 17 (8) ◽  
pp. 1484-1507 ◽  
Author(s):  
Nan Gao ◽  
Jianfeng Zhang ◽  
Mira A. Rao ◽  
Thomas C. Case ◽  
Janni Mirosevich ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Androgen Receptor ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Forkhead Box

Activation of the human dipeptidyl peptidase IV gene by hepatocyte nuclear factor-1 (HNF-1) in Caco-2 cells

1998 ◽  
Vol 114 ◽  
pp. A878
Author(s):  
Roger H. Erickson ◽  
James R. Gum ◽  
Craig D. Lotterman ◽  
James W. Hicks ◽  
Young S. Kim
Keyword(s):  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Nuclear Factor 1

scholarly journals Proteomic screen defines the hepatocyte nuclear factor 1α-binding partners and identifies HMGB1 as a new cofactor of HNF1α

2007 ◽  
Vol 36 (4) ◽  
pp. 1209-1219 ◽  
Author(s):  
Miao Yu ◽  
Jian Wang ◽  
Wei Li ◽  
Yan Zhi Yuan ◽  
Chang Yan Li ◽  
...  
Keyword(s):  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Proteomic Screen ◽  
Binding Partners ◽  
Hepatocyte Nuclear Factor 1Α
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