Comprehensive Gene Expression Profile of a Normal Human Liver

2000 ◽  
Vol 269 (1) ◽  
pp. 110-116 ◽  
Author(s):  
Taro Yamashita ◽  
Shin-ichi Hashimoto ◽  
Shuichi Kaneko ◽  
Shigenori Nagai ◽  
Nobuaki Toyoda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Human Liver ◽  
Normal Human Liver ◽  
Normal Human

scholarly journals Identification of myeloid cells in the human enthesis as the main source of local IL-23 production

2019 ◽  
Vol 78 (7) ◽  
pp. 929-933 ◽  
Author(s):  
Charlie Bridgewood ◽  
Abdulla Watad ◽  
Tobias Russell ◽  
Timothy M Palmer ◽  
Helena Marzo-Ortega ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Cell Population ◽  
Expression Profile ◽  
Myeloid Cells ◽  
Myeloid Cell ◽  
Ccr2 Gene ◽  
Similar Gene Expression Profile ◽  
Normal Human ◽  
Similar Gene

ObjectiveWe investigated whether the normal human spinal enthesis contained resident myeloid cell populations, capable of producing pivotal proinflammatory cytokines including tumour necrosis factor (TNF) and interleukin (IL)-23 and determined whether these could be modified by PDE4 inhibition.MethodsNormal human enthesis soft tissue (ST) and adjacent perientheseal bone (PEB) (n=15) were evaluated using immunohistochemistry (IHC), digested for myeloid cell phenotyping, sorted and stimulated with different adjuvants (lipopolysaccharide and mannan). Stimulated enthesis fractions were analysed for inducible production of spondyloarthropathy disease-relevant mediators (IL-23 full protein, TNF, IL-1β and CCL20). Myeloid populations were also compared with matched blood populations for further mRNA analysis and the effect of PDE4 inhibition was assessed.ResultsA myeloid cell population (CD45+ HLADR+ CD14+ CD11c+) phenotype was isolated from both the ST and adjacent PEB and termed ‘CD14+ myeloid cells’ with tissue localisation confirmed by CD14+ IHC. The CD14− fraction contained a CD123+ HLADR+ CD11c− cell population (plasmacytoid dendritic cells). The CD14+ population was the dominant entheseal producer of IL-23, IL-1β, TNF and CCL20. IL-23 and TNF from the CD14+ population could be downregulated by a PDE4I and other agents (histamine and 8-Bromo-cAMP) which elevate cAMP. Entheseal CD14+ cells had a broadly similar gene expression profile to the corresponding CD14+ population from matched blood but showed significantly lower CCR2 gene expression.ConclusionsThe human enthesis contains a CD14+ myeloid population that produces most of the inducible IL-23, IL-1β, TNF and CCL20. This population has similar gene expression profile to the matched blood CD14+ population.

Monokine gene expression in normal human liver: selective involvement of the portal compartment

2008 ◽  
Vol 12 (1) ◽  
pp. 34-41 ◽  
Author(s):  
Dominique Emilie ◽  
Emmanuelle Navratil ◽  
Odile Devergne ◽  
Michel Reynes ◽  
Marie-Claude Crevon ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Liver ◽  
Normal Human Liver ◽  
Normal Human

scholarly journals Calcitonin gene expression in normal human liver

FEBS Letters ◽  
1993 ◽  
Vol 331 (1-2) ◽  
pp. 15-18 ◽  
Author(s):  
S. Bracq ◽  
M. Machairas ◽  
B. Clement ◽  
E. Pidoux ◽  
M. Andreoletti ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Liver ◽  
Calcitonin Gene ◽  
Normal Human Liver ◽  
Normal Human

Gene Expression Profile in Persons Exposed to the WTC With and Without PTSD

2009 ◽  
Author(s):  
Rachel Yehuda ◽  
Julia Golier ◽  
Sandro Galea ◽  
Marcus Ising ◽  
Florian Holsborer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile
Sign in / Sign up

Export Citation Format

Share Document